引用本文:
陈昌云, 李小华, 单云, 夏娟, 相秉仁. 普利沙星与Co(phen)32+及DNA间结合作用的电化学和光谱[J]. 应用化学,
2006, 23(7): 818-820.
Citation: CHEN Chang-Yun, LI Xiao-Hua, SHAN Yun, XIA Juan, XIANG Bing-Ren. Studies on the Binding among Co(phen)32+,Prulifloxacin and DNA by Means of Spectroscopic and Voltammetric Methods[J]. Chinese Journal of Applied Chemistry, 2006, 23(7): 818-820.
Citation: CHEN Chang-Yun, LI Xiao-Hua, SHAN Yun, XIA Juan, XIANG Bing-Ren. Studies on the Binding among Co(phen)32+,Prulifloxacin and DNA by Means of Spectroscopic and Voltammetric Methods[J]. Chinese Journal of Applied Chemistry, 2006, 23(7): 818-820.
普利沙星与Co(phen)32+及DNA间结合作用的电化学和光谱
摘要:
利用电化学和紫外光谱法研究了邻菲咯啉合钴(Co(phen)32+)与普利沙星(PLFX)及DNA间在pH=7.4的三羟甲基氨基甲烷-盐酸缓冲溶液中的结合作用。紫外光谱测量结果表明,普利沙星与Co(phen)32+发生了结合作用形成二元络合物,结合常数为2.0×104 L/mol。电化学测量表明,在Co(phen)32+-普利沙星络合物中加入DNA后,Co(phen)32+-普利沙星络合物的峰电流下降,峰电位差增大了17 mV,式电位负移了3.5 mV,推断络合物与DNA作用方式主要为静电作用。
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关键词:
- 普利沙星
- / Co(phen)32+
- / CT-DNA
- / 结合作用
English
Studies on the Binding among Co(phen)32+,Prulifloxacin and DNA by Means of Spectroscopic and Voltammetric Methods
Abstract:
The binding among Co(phen)32+,prulifloxacin and calf thymus DNA in Tris buffer solution(pH=7.4) were investigated by means of cyclic voltammetry and UV spectroscopy.The UV results showed prulifloxacin and Co(phen)32+ formed a binary complex with a binding constant of 2.0×104 L/mol.After adding DNA,the binary complex continued to combine with DNA.The electrochemical results indicated that the peak current decreased in the presence of DNA;the peak potential difference was increased by 17 mV;formal potential shifted 3.5 mV negatively.The results show that the binding mode of Co(phen)32+-prulifloxacin binary complex and DNA was mainly electrostatic interaction.
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Key words:
- prulifloxacin
- / Co(phen)32+
- / CT-DNA
- / binding
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