引用本文:
柳畅先, 韦喜勤. 酶法测定乙醛[J]. 应用化学,
2000, 17(4): 422-423.
Citation: LIU Chang-Xian, WEI Xi-Qin. Determination of Acetaldehyde by Enzymatic Method[J]. Chinese Journal of Applied Chemistry, 2000, 17(4): 422-423.
Citation: LIU Chang-Xian, WEI Xi-Qin. Determination of Acetaldehyde by Enzymatic Method[J]. Chinese Journal of Applied Chemistry, 2000, 17(4): 422-423.
酶法测定乙醛
English
Determination of Acetaldehyde by Enzymatic Method
Abstract:
An enzymo-spectrophotometric method was proposed based on the catalytic reaction of acetaldehyde and nicotinamide adenine dinucleotide(NADH) by alcohol dehydrogenase(ADH). The change of the absorbance of NADH was determined for calculating the velocity of the enzymatic reaction. Linear calibration graphs were obtained for acetaldehyde in the range of 0.2~10 mg/L, with a detection limit of 0.16 mg/L. The effects of the pH and interferences from metal cations, formaldehyde and n-butylaldehyde on the reaction were examined. The method was simple, rapid and sensitive.
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Key words:
- acetaldehyde
- / enzymatic determination
- / alcohol dehydrogenase
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