引用本文:
望天志, 吴鼎泉, 万洪文, 屈松生. 用微量热法研究漆酶和3,4-二羟基苯甲醛的反应[J]. 应用化学,
1997, 14(2): 66-68.
Citation: Wang Tianzhi, Wu Dingquan, Wan Hongwen, Qu Songsheng. Study on the Reaction Between Laccase and 3,4-Dihydroxybenzaldehyde by Microcalorimetry[J]. Chinese Journal of Applied Chemistry, 1997, 14(2): 66-68.
Citation: Wang Tianzhi, Wu Dingquan, Wan Hongwen, Qu Songsheng. Study on the Reaction Between Laccase and 3,4-Dihydroxybenzaldehyde by Microcalorimetry[J]. Chinese Journal of Applied Chemistry, 1997, 14(2): 66-68.
用微量热法研究漆酶和3,4-二羟基苯甲醛的反应
摘要:
近几年来,对漆酶的活性中心——铜的结构及其作用机制进行了大量的研究[1,2].
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关键词:
- 漆树漆酶
- / 微量热法
- / 3.4-二羟基苯甲醛
English
Study on the Reaction Between Laccase and 3,4-Dihydroxybenzaldehyde by Microcalorimetry
Abstract:
Thermokinetic properties of the reaction between laccase and 3,4-dihydroxyben-zaldehyde have been studied with a microcalorimetry system. The molar reaction enthalpy(ΔrHm), michaelis constant(Km), rate constant(k2), binding energy(ΔG0), activation gibbis free energy(ΔG=) and laccase activity(EA) have been determined. The result has been discussed by the transition state theory.
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Key words:
- laccase
- / microcalorimetry
- / thermokinetics
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