Citation: Kanyu Xun, Yue Sun, Yue Zhang, Liping Qiu. Functional Nucleic Acid-Based Fluorescence Cell Imaging[J]. Chemistry, ;2021, 84(2): 98-107. shu

Functional Nucleic Acid-Based Fluorescence Cell Imaging

1.
State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha, 410082
2.
Key Laboratory of Birth Defect Research and Prevention, National Health Commission, Changsha, 410000

Corresponding author: Liping Qiu, qiuliping@hnu.edu.cn
Received Date: 11 September 2020
Accepted Date: 4 November 2020

Figures(8)

Cell is the structural and functional unit of organisms. Studying the spatiotemporal distribution and activity of specific biomolecules in living cells would provide valuable information in understanding various biological processes. Fluorescence imaging technology has showed great potential for live-cell imaging. On the other hand, development of high-performance fluorescent probes has become a major bottleneck in this field. Functional nucleic acids (FNAs) are a class of oligonucleotides with specific chemical and/or biological functions, including natural ribozymes and riboswitches, as well as aptamers and DNAzymes screened through the SELEX (systematic evolution of ligands by exponential enrichment) technique. Taking advantages of simple synthesis, low immunogenicity, small size, high chemical stability, easy modification, FNAs have received extensive attention in the fields of bio-analysis and bio-imaging. This review summarizes the recent applications of FNA in fluorescence cell imaging, and points out the challenges and future prospects in this field.
- Functional nucleic acids,
- Fluorescence probe,
- Cell imaging,
- Bioanalysis
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