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Citation: Lv Haijun, Kang Caihong, Liu Lei, Yu Yifeng, Chen Aibing, Liu Honghou, Liu Huan, Zhang Huanpeng, Kang Xiao. Study on the Enzymatic Synthesis of Cefprozil[J]. Chemistry, ;2017, 80(9): 858-862. shu

Study on the Enzymatic Synthesis of Cefprozil

Lv Haijun ¹ ,
Kang Caihong ¹ ,
Liu Lei ^1,2,, ,
Yu Yifeng ¹ ,
Chen Aibing ¹ ,
Liu Honghou ³ ,
Liu Huan ¹ ,
Zhang Huanpeng ¹ ,
Kang Xiao ¹

1.
Hebei Research Center of Pharmaceutical and Chemical Engineering, College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018
2.
Hebei Key Laboratory of Molecular Chemistry for Drug, Hebei Research Center of Pharmaceutical and Chemical Engineering, Shijiazhuang 050018
3.
Hebei Huashi Bio-Tech Co. Ltd, Shijiazhuang 050035

Corresponding author: Liu Lei, liulei@hebust.edu.cn
Received Date: 1 March 2017
Accepted Date: 3 May 2017

Figures(8)

Cefprozil was synthesized using 7-amino-3-[(Z)-all-1-yl]-3-cephem-4-carboxylic acid (7-APCA) as the nucleus, D-hydroxyphenylglycine methyl ester (D-HPGME) as acyl donor, water as solvent via the enzymatic method under the catalysis of immobilized penicillin G acylase. In the study, the carrier of the catalytic enzyme is amino and epoxy polymer. The research screened the appropriate enzyme activity and investigated the influence of several factors including pH, temperature, enzyme dosage, feed ratio and substrate concentration on the synthesis of cefprozil. The data obtained through optimized experiment of synthesizing cefprozil by penicillin G acylase (PGA) lay the foundation for the implementation of the green production technology of cefprozil.
- Cefprozil,
- Penicillin G acylase,
- Synthetic hydrolysis ratio,
- Yield
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