Citation: Ma Zhiying, Gao Xue, Song Yu, Liu Xiuying, Li Jianrong. Determination of Free Cholesterol in Serum by Tyramine Modified Graphene Quantum Dots[J]. Chemistry, ;2020, 83(7): 659-664. shu

Determination of Free Cholesterol in Serum by Tyramine Modified Graphene Quantum Dots

Engineering and Technology Research Center of Food Preservation, Processing and Safety Control of Liaoning Province, Liaoning Provincial Key Laboratory of Food Quality Safety and Functional Food, College of Food Science and Engineering, Bohai University, Jinzhou, 121013

Corresponding author: Gao Xue, huaxue_gaoxue@126.com Li Jianrong, lijr6491@163.com
Received Date: 29 November 2019
Accepted Date: 26 January 2020

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In this study, graphene quantum dots (GQDs) with catalase activity were synthesized from citric acid. GQDs modified by tyramine (TYR-GQDs) can reduce hydrogen peroxide to hydroxyl radicals. GQDs aggregates through the cross-linking between phenolic hydroxyls, which leads to fluorescence quenching. Since cholesterol oxidase can catalyze the oxidation of cholesterol to produce H₂O₂, a fluorescence sensor for detecting cholesterol was established based on TYR-GQDs. The experimental results showed that under the condition of pH=7.4, the fluorescence quenching rate of TYR-GQDs exlibits a good linear relationship with the logarithm of cholesterol concentration (2.67×10^-8~2.67×10^-3 mol/L), and the detection limit is 9.32×10^-9 mol/L. The interference experiment showed that the fluorescent sensor has high selectivity for cholesterol and can be used for the detection of free cholesterol in human serum. The recovery of standard addition is 96.55%~100.14%.
- TYR-GQDs,
- hydrogen peroxide,
- cholesterol
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