Citation: Jun GAO, Yuan Zong LI, Zhen Quan GUO, Wen Bac CHANG, Yun Xiang CI. Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone[J]. Chinese Chemical Letters, ;1999, 10(6): 495-498. shu

Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone

1.
Department of Chemistry, Peking University, Beijing 100871;
2.
Department of Biology, Peking University, Beijing 100871

Received Date: 4 January 1999

Fund Project: This work is supported by NSFC (29775002).

A novel immunoassay method for methyltestosterone (MT) in serum was developed. The antigen of MT (Ag_MT)was synthesized by covalently bonding MT to BSA and raising its antibody (Ab_MT). Ab_MT was conjugated to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab_MT bound thermally reversible hydrogel p-NIPAAm-Ab_MT. A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag_MT) and free MT with limited amount of p-NIPAAm-Ab_MT was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50 ng/ml. The recoveries of MT from human serum are satisfactory.
- Methyltcstosterone,
- immunoassay,
- poly-N-isopropylacrylamide,
- thermally reversible hydrogel
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