Citation: Hai Liang CUI, Yong YU, Wan Chun CHEN, Qi KANG. Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method[J]. Chinese Chemical Letters, ;2006, 17(1): 101-104. shu

Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method

Hai Liang CUI ^1, ,
Yong YU ¹ ,
Wan Chun CHEN ^1,2 ,
Qi KANG ¹

1.
National Microgravity Lab, Institute of Mechanics, Chinese Academy of Science, Beijing 100080;
2.
Institute of Physics, Chinese Academy of Science, Beijing 100080

Received Date: 17 May 2005

Fund Project: The work was supported by the National Natural Science Foundation of China (No. 10472127 and No.10432060), and Knowledge Innovation Program of Chinese Academy of Sciences (KSCX2 - SW - 322, KJCX2-SW-L05).

The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaC1, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other.The higher the concentrations of lysozyme and NaC1 were, the faster the growth rate of (110) face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy (α) of growing steps were calculated about 4.01×l0^-8 J·cm^-2 according to the model of multiple two-dimensional nucleation mechanism.
- Protein crystal,
- growth rate,
- dynamic light scattering,
- lysozyme
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