Citation: Fei WANG, Xiong CHEN, Qian CHEN, Xing Chen QIN, Zhi Xiao LI. Determination of Neurotoxin β-ODAP and Non-protein Amino Acids in Lathyrus Sativus by High-Performance Liquid Chromatography with Precolumn Derivatization with 6-Amino quinolyl-N-hydroxysuccinimidyl Carbamate (AQC)[J]. Chinese Chemical Letters, ;2000, 11(7): 605-608. shu

Determination of Neurotoxin β-ODAP and Non-protein Amino Acids in Lathyrus Sativus by High-Performance Liquid Chromatography with Precolumn Derivatization with 6-Amino quinolyl-N-hydroxysuccinimidyl Carbamate (AQC)

a National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000;
b State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000

Received Date: 31 December 1999
Revised Date: 6 April 2000

Fund Project: The project(39770469) is supported by the National Natural Science Foundation of China.

A new method was developed for the quantitative determination of the neurotoxic non-protein amino acid, 3-N-oxalyl-L-2,3-diaminopropionic acid (β-ODAP), its nontoxic α-isomer and other non-protein amino acids in the plant samples of Lathyrus sativus after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) by reversed-phase high-performance liquid chromatography (HPLC). 2-Amino butyric acid (ABA) was used as an internal standard. The RP HPLC detection limit for both isomers is 1.8 ng with good response linearity. The results are compared with a colorimetric method.
- RP HPLC,
- Lathyrus sativus,
- 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
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