Citation: Shao Ming YANG, Yang Mei LI, Xiu Ming JIANG, Xian Fu LIN. A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion[J]. Chinese Chemical Letters, ;2005, 16(7): 983-986. shu

A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion

Department of Chemistry, Zhejiang University, Hangzhou 310027

Received Date: 16 August 2004

Fund Project: This research has been supported by International Coorperation Project of Zhejiang Science and Technology Bureau (021106032).

A novel reagentless biosensor constructed by the organic dye nile blue (NB) and horseradish peroxidase (HRP) has been fabricated via layer-by-layer (LBL) self-assembly technique. NB premixed with polyanion poly (sodium-p-styrenesulfonate) (PSS) acts as the mediator between the immobilized HRP and the electrode surface. The response of the biosensor to hydrogen peroxide has been investigated. The linear range of the biosensor to hydrogen peroxide was from 0.20 mmol/L to 7.03 mmol/L with a sensitivity of 8.45 μA/(mmol/L).
- Nile blue,
- premixed,
- layer-by-layer,
- horseradish peroxidase (HRP),
- biosensor
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