Citation: Jin YAN, Jie Bo MI, Wen Bao CHANG. Study of Immunoassay Methods for Recombinant Human Erythropoietin (rhEPO) Using Competitive ELISA[J]. Chinese Chemical Letters, ;2004, 15(8): 939-942. shu

Study of Immunoassay Methods for Recombinant Human Erythropoietin (rhEPO) Using Competitive ELISA

The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871

Received Date: 21 July 2003

Fund Project: This work is financially supported by the National Natural Science Foundation of China (Grants No.B050305).

Two different immunoassay methods, competitive indirect enzyme-linked immuno-sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.
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