Citation: Fei WANG, Xiong CHEN, Qian CHEN, Xin Chen QIN, Zhi Xiao LI. HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene[J]. Chinese Chemical Letters, ;2000, 11(5): 435-438. shu

HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene

a National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000;
b State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000

Received Date: 1 November 1999
Revised Date: 25 February 2000

Fund Project: The project (39770469) supported by the National Natural Science Foundation of China.

A rapid and simple method is presented for determining β-N-oxalyl-α,β-diaminopropionic acid (β-ODAP) and its much less toxic α-isomer (α-ODAP) in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatographic method for the separation of the derivatives in the pmol range is reported.
- HPLC,
- Lathyrus sativus,
- 1-fluoro-2,4-dinitrobenzene,
- α-and β-N-oxalyl-α,β-diamino propionic acid,
- neurotoxin
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