Citation: Qi Danping, Lin Jiangli, Gao Min, Lu Jishuai. Pectinase Immobilization Using HPD-750 Macroporous Resin as Carrier Material[J]. Chemistry, ;2020, 83(2): 161-166. shu

Pectinase Immobilization Using HPD-750 Macroporous Resin as Carrier Material

College of Chemistry and Chemical Engineering, Xinjiang University, Urumchi, 830046

Corresponding author: Lin Jiangli, 736058563@qq.com
Received Date: 2 August 2019
Accepted Date: 30 November 2019

Figures(9)

HPD-750 resin is a medium-polar macroporous adsorption resin with good biocompatibility, stable mechanical properties and large specific surface area, which can be used for immobilizing enzyme. In this paper, pectinase was immobilized by HPD-750 macroporous resin, and the effects of various factors on the immobilized enzyme were studied. The optimized immobilization parameters are:pH 4.0, the immobilization temperature 45℃, the immobilization time 4h, and the amount of enzyme added 0.16g/mL, Under these conditions, the immobilized enzyme activity can reach 5146U/mg. Compared with free pectinase, the immobilized enzyme was found to exhibit better stability of pH and thermo. Additionally, the immobilized pectinase had remarkable reusability and storage stability, the enzyme activity remained more than 80% after 10 cycles of use; and after storage at 4℃ for 25 days, the enzyme activity remained more than 60%. Compared with immobilized enzyme prepared D311 macroporous resin, polyacrylamide and sodium alginate microspheres, the HPD-750 immobilized enzyme has better activity, operational stability, mechanical stability and storage stability. Hence that the HPD-750 macroporous resin could be used as a good carrier material for immobilized enzymes.
- HPD-750 macroporous resin,
- Carrier material,
- Pectinase,
- Immobilized
1. [1]
  Rajdeo K, Harini T, Lavanya K, et al. Food Bioprod. Process., 2016, 99: 12~19.
2. [2]
  Schols H A, Bakx E J, Schipper D, et al. Carbohyd. Res., 1995, 279: 265~279.
3. [3]
4. [4]
5. [5]
6. [6]
  Van der Vlugt-Bergmans C J B, Meeuwsen P J A, Voragen A G J, et al. Appl. Environ. Microbiol., 2000, 66(1): 36~41.
7. [7]
8. [8]
  Demir N, Acar J, Sarıoğlu K, et al. J. Food Eng., 2001, 47(4): 275~280.
9. [9]
  Li T, Wang N, Li S, et al. Biotechnol. Lett., 2007, 29(9): 1413~1416.
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
  Lei Z, Jiang Q. J. Agric. Food Chem., 2011, 59(6): 2592~2599.
23. [23]
24. [24]
25. [25]
  Li Y, Liu J, Zhang K, et al. Ind. Eng. Chem. Res., 2017, 57(2): 559~567.
26. [26]
27. [27]
1. [1]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
2. [2]
  Ningyuan Chen , Qingyi Zeng , Zhiqiang Kuang , Peicong Tian , Dazhi Yan , Weiliang Jin , Deming Kong . Digital-Assisted Experimental Study on Dye Adsorption by Porous Materials. University Chemistry, 2026, 41(1): 321-331. doi: 10.12461/PKU.DXHX202504072
3. [3]
  Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
4. [4]
  Xiaoqi LAN , Wei LI , Deyi YANG , Hao WANG , Zheng LIU , Rongting GUO , Qizhi CHEN . Preparation and electrochemical performance of “sandwich structured” MXene Ti₃C₂T_x/hollow ZIF-67 sulfur host composites. Chinese Journal of Inorganic Chemistry, 2026, 42(4): 760-772. doi: 10.11862/CJIC.20250273
5. [5]
  Qiaorong RU . Synthesis and characterization of tripyridine functionalized polyionic liquid luminescent materials. Chinese Journal of Inorganic Chemistry, 2026, 42(1): 111-119. doi: 10.11862/CJIC.20250121
6. [6]
  Guangming YIN , Huaiyao WANG , Jianhua ZHENG , Xinyue DONG , Jian LI , Yi'nan SUN , Yiming GAO , Bingbing WANG . Preparation and photocatalytic degradation performance of Ag/protonated g-C₃N₄ nanorod materials. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1491-1500. doi: 10.11862/CJIC.20240086
7. [7]
  Fugui XI , Du LI , Zhourui YAN , Hui WANG , Junyu XIANG , Zhiyun DONG . Functionalized zirconium metal-organic frameworks for the removal of tetracycline from water. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 683-694. doi: 10.11862/CJIC.20240291
8. [8]
  Mengyang LI , Hao XU , Zhonghao NIU , Chunhua GONG , Weihui ZHONG , Jingli XIE . Highly effective catalytic synthesis of β-amino alcohols by using viologen-polyoxometalate hybrid materials. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1294-1300. doi: 10.11862/CJIC.20250080
9. [9]
  Xiangyu CHEN , Zhenzhen MIAO , Ligang XU , Guangbao WU , Zhuang LIU , Wenzhen LÜ , Runfeng CHEN . Research progress on low-dimensional organic-inorganic hybrid metal halide optoelectronic materials. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2201-2217. doi: 10.11862/CJIC.20250056
10. [10]
  Wei Li , Han Xu , Chuancan Gu , Ziyan Liu , Yan'an Li , Yan Geng . Digital Experiment on Nano-COF Materials Modulating Intracellular Ca²⁺ Concentration to Enhance Photodynamic Therapy. University Chemistry, 2026, 41(1): 354-362. doi: 10.12461/PKU.DXHX202506001
11. [11]
  Yihui Song , Shangshang Qin , Kai Wu , Chengyun Jin , Bin Yu . 生物化学在高水平创新型药学人才培养中的交叉融合应用——以去甲基化酶LSD1抑制剂的活性评价为例. University Chemistry, 2025, 40(6): 341-352. doi: 10.12461/PKU.DXHX202406018
12. [12]
  Yinyin Qian , Rui Xu . Utilizing VESTA Software in the Context of Material Chemistry: Analyzing Twin Crystal Nanostructures in Indium Antimonide. University Chemistry, 2024, 39(3): 103-107. doi: 10.3866/PKU.DXHX202307051
13. [13]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
14. [14]
  Hongpeng He , Mengmeng Zhang , Mengjiao Hao , Wei Du , Haibing Xia . Synthesis of Different Aspect-Ratios of Fixed Width Gold Nanorods. Acta Physico-Chimica Sinica, 2024, 40(5): 2304043-0. doi: 10.3866/PKU.WHXB202304043
15. [15]
  Yue Wu , Jun Li , Bo Zhang , Yan Yang , Haibo Li , Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028
16. [16]
  Ruiqing LIU , Wenxiu LIU , Kun XIE , Yiran LIU , Hui CHENG , Xiaoyu WANG , Chenxu TIAN , Xiujing LIN , Xiaomiao FENG . Three-dimensional porous titanium nitride as a highly efficient sulfur host. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 867-876. doi: 10.11862/CJIC.20230441
17. [17]
  Xuejie Wang , Guoqing Cui , Congkai Wang , Yang Yang , Guiyuan Jiang , Chunming Xu . Research Progress on Carbon-based Catalysts for Catalytic Dehydrogenation of Liquid Organic Hydrogen Carriers. Acta Physico-Chimica Sinica, 2025, 41(5): 100044-0. doi: 10.1016/j.actphy.2024.100044
18. [18]
  Dan LUO , Xingcheng LIU , Dong LI , Tong CHANG . Metal-support interaction effects on CO activation over Co_n/SiO₂ catalysts. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2337-2344. doi: 10.11862/CJIC.20250003
19. [19]
  Xinghai Liu , Hongke Wu . Exploration and Practice of Ideological and Political Education in Heterocyclic Chemistry Based on "Fentanyl" Event. University Chemistry, 2024, 39(8): 359-364. doi: 10.3866/PKU.DXHX202312100
20. [20]
  Qiang MA , Yiming ZHU , Meiqi HUA , Guangyu LU , Xingdong WANG , Hailong YU , Huan PANG , Yuping LI . Research progress on metal-organic frameworks as comprehensive carrier platforms for delivering anti-glioma drugs. Chinese Journal of Inorganic Chemistry, 2026, 42(4): 657-667. doi: 10.11862/CJIC.20250250

Get Citation

PDF

XML

Metrics

PDF Downloads(17)
Abstract views(2198)
HTML views(508)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142