Citation: Lv Jubo, Zhang Yahui, Liu Gang, Xu Hui. Amplification Detection of Adenosine-Triphosphate Based on Hybrid Chain Reaction and Auxiliary Magnetic Sensing Strategy[J]. Chemistry, ;2018, 81(1): 59-64, 76. shu

Amplification Detection of Adenosine-Triphosphate Based on Hybrid Chain Reaction and Auxiliary Magnetic Sensing Strategy

School of Chemistry and Materials Science, Ludong University, Yantai 264025

Received Date: 23 August 2017
Accepted Date: 31 October 2017

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We put forward a hybrid chain reaction amplification detection of adenosine triphosphate (ATP) based on auxiliary magnetic sensing strategy. The surface of magnetic nanoparticles is easy to be modified, and they have the advantages of the convenient operation, the good separation effect and the higher selectivity for biological sensing. First of all, the biotin labeled ATP aptamer will connect to the surface of the magnetic nanoparticles which is modified with streptavidin based on the affinity between biotin and streptavidin. Then a single DNA which is complementary to the ATP aptamer is added to hybrid with ATP aptamer, and the unnecessary DNA will be removed by magnetic separation. When the targeted ATP is added to the solution, the ATP will bind with its aptamer specially by releasing the complementary single-stranded DNA, and the complementary single-stranded DNA will continue to be used for the next step of hybridization chain reaction. The signal would be stronger after magnetic separation. The background will be reduced by the fluorescence quenching effect of the graphene oxide, and the method has high sensitivity and high selectivity for targeting ATP. The lowest detection concentration of ATP is 0.1nmol/L.
- Graphene oxide,
- Hybridization chain reaction,
- Magnetic auxiliary,
- Fluorescent signal amplification
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