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基质辅助激光解吸电离飞行时间质谱分析低分子量化合物的基质的研究进展

陈秀英 ,  高保祥 ,  周焕英

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引用本文: 陈秀英, 高保祥, 周焕英. 基质辅助激光解吸电离飞行时间质谱分析低分子量化合物的基质的研究进展[J]. 分析化学, 2022, 50(1): 12-24. doi: 10.19756/j.issn.0253-3820.211012 shu
Citation:  CHEN Xiu-Ying,  GAO Bao-Xiang,  ZHOU Huan-Ying. Recent Progress in Matrix for Analysis of Low Molecular Weight Compounds Using Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2022, 50(1): 12-24. doi: 10.19756/j.issn.0253-3820.211012 shu

基质辅助激光解吸电离飞行时间质谱分析低分子量化合物的基质的研究进展

  • 1.

    河北大学化学与环境科学学院 药物化学与分子诊断教育部重点实验室, 保定 071002;

  • 2.

    军事科学院军事医学研究院 环境医学与作业医学研究所, 天津 300050

    • 通讯作者: 高保祥,E-mail:bxgao@hbu.edu.cn; 周焕英,E-mail:zhouhytj@163.com
  • 基金项目:

    国家重点研发计划项目（No.2017YFF0211301）资助。

摘要: 基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）作为一种软电离质谱技术，在蛋白质、多肽、核酸等大分子的分析中得到了广泛应用。然而，用于MALDI-TOF MS检测所使用的传统基质在低分子量区域存在基质相关峰干扰、基质/分析物共结晶均匀性差等问题，从而干扰低分子量化合物（LMWC）的检测。近年来，研究者针对以上问题开发了多种适于LMWC检测的新型MALDI基质。本文从新型无机材料类基质、有机化合物类基质和其它类型基质（金属有机框架类、离子液体基质、反应性基质等）等方面评述了近十年MALDI-TOF MS分析LMWC的研究进展，重点介绍了相关基质的制备、特点和应用，并对未来发展趋势进行了展望。

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