Citation: Xing Jiehua, Yin Ting, Luo Yingmei, Xiao Le, Lv Yingnian, Cai Lintao, Zheng Mingbin. Nano-Fluorescence Labeling of Salmonella Based on Michael Addition Reaction[J]. Chemistry, ;2020, 83(2): 127-131, 155. shu

Nano-Fluorescence Labeling of Salmonella Based on Michael Addition Reaction

Xing Jiehua ¹ ,
Yin Ting ² ,
Luo Yingmei ¹ ,
Xiao Le ¹ ,
Lv Yingnian ^1,, ,
Cai Lintao ^2,, ,
Zheng Mingbin ^1,2,,

1.
College of Pharmacy of Guangdong Medical University, Institute of Marine Medicine of Guangdong Medical University, Guangdong Key Laboratory for Research and Development of Natural Drugs, Zhanjiang, 524023
2.
CAS Key of Laboratory of Health Informatics, Guangdong Key Laboratory of Nanomedicine, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055

Corresponding author: Lv Yingnian, lyn7591@hotmail.com Cai Lintao, lt.cai@siat.ac.cn Zheng Mingbin, mb.zheng@siat.ac.cn
Received Date: 24 July 2019
Accepted Date: 20 October 2019

Figures(5)

In this paper, Michael addition reaction was successfully applied to construct nano-fluorescent probe-labeled Salmonella (YB1-INPs). The mild reduction of the disulfide bond (S-S) on the outer membrane protein surface of Salmonella YB1 was achieved by tris(2-carboxyethyl) phosphine, followed by crosslinking with maleimide group (Mal) of indocyanine green (ICG)@nanoprobe (INPs). The morphological and optical properties of YB1-INPs were characterized by scanning electron microscopy (SEM) and confocal fluorescence imaging. The activity of YB1-INPs was investigated by live/dead baclight bacterial viability kit and LB agar plate test. The results showed that nanoprobe INPs were successful labeled on the surface of YB1, while this mild chemical labeling had no adverse effect on the appearance, vitality and growth rate of YB1, and could be used for the fluorescence distribution imaging of Salmonella YB1 in vivo. This simple, safe and efficient fluorescence labeling method provided by this study can label nanomaterials on biological carriers, applying to drug delivery and in vivo monitoring of biological carriers.
- Salmonella YB1,
- Nanoprobe,
- Fluorescence labeling,
- Disulfide bond,
- Mild reduction
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