Citation: Tong LIU, Xin Du GENG. The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography[J]. Chinese Chemical Letters, ;1999, 10(3): 219-222. shu

The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography

Tong LIU ,
Xin Du GENG

Institute of Modem Separation Science, Shaanxi Provincial Key Laboratory of Modern Separation Science, Northwest University, Xi'an 710069

Received Date: 26 October 1998

Fund Project: This work was supported by grants from National Natural Science Foundation of China

The separation efficiency of biopolymers with a short column in liquid chromatography has been investigated in this paper.It was found that the column length has slight effect on the resolution of biopolymers under gradient elution.The reasons have been explained by stoichiometric displacement model for retention of solute.The column 1 .0 cm long was also used in the separation and purification of recombinant human granulocyte colony-simulating factor (rhG-CSF).It only took 40 min and the purity by one step was found to be almost 100%.
- Short column,
- biopolymer,
- stoichiometric displacement model for retention,
- Z value
1. [1]
  Yue Ming Zhou , Jian Hua Ding , Xie Zhang , Huan Wen Chen . Ion-ion reactions for charge reduction of biopolymer at atmospheric pressure ambient. Chinese Chemical Letters, 2007, 18(1): 115-117. doi: 10.1016/j.cclet.2006.11.010
2. [2]
  Fu An WANG , Chang Sheng YANG , Wen Chang WANG , Yuan Li JIANG . INTERSECTION POINT RULE OF THE RETENTION VALUE AND NORMAL BOILING POINT OF THE HOMOLOGUES IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. Chinese Chemical Letters, 1991, 3(8): 637-640.
3. [3]
  Xin Du GENG , Liu Jiao BIAN . A UNIFIED RETENTION MODEL OF SOLUTE IN LIQUID CHROMATOGRAPHY. Chinese Chemical Letters, 1990, 1(2): 135-138.
4. [4]
  Mei Qingqing , Shen Xiaojun , Liu Huizhen , Han Buxing . Selectively transform lignin into value-added chemicals. Chinese Chemical Letters, 2019, 30(1): 15-24. doi: 10.1016/j.cclet.2018.04.032
5. [5]
  Lan Yu , Liu Xiaohua . Introduction to the special column on organocatalysis. Chinese Chemical Letters, 2018, 29(8): 1179-1180. doi: 10.1016/j.cclet.2018.07.009
6. [6]
  Zidong Wei , Minhua Shao . Preface to Special Column on Electrocatalysis. Chinese Journal of Catalysis, 2020, 41(5): 731-731. doi: 10.1016/S1872-2067(20)63558-6
7. [7]
  Ke Qiang DING , Qing Fei WANG , Ru Ting TONG , Hui Bo SHAO . Capacitance Characterization of the Effect of pH Value on the Self-assembled Monolayers of Octadecanethiol. Chinese Chemical Letters, 2003, 14(4): 411-412.
8. [8]
  ZHANG Hong-guang , WANG Wen-tai , FENG Li-juan , LI Chun-hu , WANG Liang . Effect of hydrothermal pH value on composition and morphology of bismuth oxybromide and their photocatalytic performance. Journal of Fuel Chemistry and Technology, 2019, 47(5): 582-589.
9. [9]
  Yu Ting ZHAO , De Hong CHEN , Dai Rong CHEN , Xiu Ling JIAO . Synthesis of Monodisperse α-Fe₂O₃ Particles in Short Periods. Chinese Chemical Letters, 2003, 14(3): 323-326.
10. [10]
  Chen Qian , Cheng Xuanrui , Long Huimin , Rao Yongfang . A short review on recent progress of Bi/semiconductor photocatalysts: The role of Bi metal. Chinese Chemical Letters, 2020, 31(10): 2583-2590. doi: 10.1016/j.cclet.2020.08.018
11. [11]
  Panagiotis Tsiakaras , Shuqin Song . Preface to Special Column on Electrocatalysis for Fuel Cells. Chinese Journal of Catalysis, 2015, 36(4): 457-457. doi: 10.1016/S1872-2067(15)60832-4
12. [12]
  Ying Wan , Serge Kaliaguine , Dongyuan Zhao . Preface to Special Column on New Porous Catalytic Materials. Chinese Journal of Catalysis, 2016, 37(1): 1-2. doi: 10.1016/S1872-2067(15)61027-0
13. [13]
  Ling Xin CHEN , Guo An LUO , Tao WEN . Electroosmotic Driving Liquid Using Nanosilica Packed Column. Chinese Chemical Letters, 2005, 16(6): 809-811.
14. [14]
  Xiao Wei Zhou , Hui Ming Jin , Lin Nan Zhang . Influence of pH value on microstructure and thermal stability of Ni-P electroless coating prepared in acidic condition. Chinese Chemical Letters, 2009, 20(7): 845-848. doi: 10.1016/j.cclet.2009.01.040
15. [15]
  Zhen Ku GUO , Lan Xiang MENG , Xin Yu REN , Ya Zhong ZHANG . APPLICATION OF THERMAL ANALYSIS TO THE QUICK DETERMINATION OF THE PROXIMATE ANALYSIS ITEMS AND CALORIC VALUE FOR COAL. Chinese Chemical Letters, 1993, 4(10): 921-924.
16. [16]
  Zhi Wei WANG , Shao Bai LI . A Convenient and Stereospecific Synthesis of (Z) Benzylidenephthalides. Chinese Chemical Letters, 2001, 12(11): 955-956.
17. [17]
  Chang Qiu ZHAO , Xian HUANG . Stereoselective Synthesis of (Z)-β-Aryltellurocinnamic Esters. Chinese Chemical Letters, 1996, 7(12): 1083-1084.
18. [18]
  LU Xu , ZHANG Yi , WU Xiao-Ting , GUO Ze-Bin , LIN Shan , ZHENG Bao-Dong . Effect of Specific Structure of Lotus Seed Oligosaccharides on the Production of Short-chain Fatty Acids by Bifidobacterium adolescentis. Chinese Journal of Structural Chemistry, 2015, 34(4): 510-522. doi: 10.14102/j.cnki.0254-5861.2011-0521
19. [19]
  Shi Xiaodong , Jiang Yixiang , Yang Dan , Zhao Hui , Tian Yuan , Li Zigang . Reversibly switching the conformation of short peptide through in-tether chiral sulfonium auxiliary. Chinese Chemical Letters, 2018, 29(3): 485-488. doi: 10.1016/j.cclet.2017.07.003

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