Citation: Ye Hua SHEN, Hai Bo WANG, Quan BAI, Xin Du GENG. Comparison of the Contributions of Tetrahydrofurfuryl Alcohol and PEG to α-Chymotrypsin Renaturation with High Performance Hydrophobic Interaction Chromatography[J]. Chinese Chemical Letters, ;2003, 14(3): 294-297. shu

Comparison of the Contributions of Tetrahydrofurfuryl Alcohol and PEG to α-Chymotrypsin Renaturation with High Performance Hydrophobic Interaction Chromatography

Institute of Modern Separation Science, Shanxi Provincial Key Laboratory of Modern Separation Science, Northwest University, Xi'an 710069

Received Date: 1 April 2002

Fund Project: These projects No. 39880003 and 20175016 were supported by the National Natural Science Foundation of China.

The contributions of tetrahydrofurfuryl alcohol (THFA) and polyethylene glycol (PEG) to the renatured efficiency of α-chymotrypsin were investigated and compared with each other. The maximum increments of bioactivity recovery of α-Chy were found to be 25.1% for THFA, 10.4% for PEG, respectively. The experimental results indicated that the denaturant solution containing THFA contributed more to the renaturation of α-Chy in high performance hydrophobic interaction chromatography (HPHIC) than that containing PEG, when the concentration of THFA was 3.2%, the bioactivity recovery of α-Chy is the highest.
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