Citation: Li Xiaolu, Guo Jing, Zhai Qian, Yi Gang. The Applications of Isothermal Nucleic Acid Amplification Technique in Electrochemical Biosensors[J]. Chemistry, ;2016, 79(12): 1127-1133. shu

The Applications of Isothermal Nucleic Acid Amplification Technique in Electrochemical Biosensors

1.
College of Laboratory Medicine, Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016

Corresponding author: Yi Gang,
Received Date: 16 May 2016
Available Online: 1 August 2016

The detection of biomolecules in the fields of clinical diagnosis, gene therapy and mutation analysis has become increasingly important. Therefore, it is significant for establishing a simple, rapid and sensitive detection method. Electrochemical biosensor has gained increasing interest because of its advantages such as simplicity, portability, easy to operate and low cost in the field of biomolecules detection. In order to improve the sensitivity of biosensors, different isothermal nucleic acid amplification techniques have been used in the construction of electrochemical biosensors. In this paper, we introduced the principle of electrochemical sensor briefly, summarize the main isothermal nucleic acid amplification techniques applied in electrochemical biosensors emphatically and compare the advantages and disadvantages of each technique.
- Isothermal nucleic acid amplification technique,
- Electroanalysis,
- Biosensor,
- Biodetection
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沈阳化工大学材料科学与工程学院沈阳 110142