Citation: Xu Xiangyu, Wang Hao, Du Zhongyu, Mao Xuyan, Jiang Liang, Sun Dezhi. Study on the Interaction of Ag Nanoparticles with Human Serum Albumin[J]. Chemistry, ;2016, 79(3): 279-282,231. shu

Study on the Interaction of Ag Nanoparticles with Human Serum Albumin

Xu Xiangyu ¹ ,
Wang Hao ¹ ,
Du Zhongyu ² ,
Mao Xuyan ¹ ,
Jiang Liang ¹ ,
Sun Dezhi ³

1.
1. Bio-Nano & Medical Engineering Institute, Jining Medical University, Jining 272067;
2.
2. Foundation College, Jining Medical University, Jining 272067;
3.
3. College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059

Received Date: 30 June 2015
Available Online: 2 November 2015
Fund Project:

Ag nanoparticles (Ag NPs) were synthesized by the chemical reduction method and the morphology of them was observed by using SEM and TEM. Interaction of the Ag NPs with human serum albumin (HSA) in aqueous solution had been investigated by UV-Vis absorption spectroscopy, fluorescence (FL) spectroscopy and SEM. The absorbance of the mixture solution was progressively increased, but the fluorescence intensity was decreased with increasing concentration of Ag NPs. The results showed that there were interaction between the Ag NPs and HSA. Furthermore, the morphology change of the Ag NPs in the mixed solution investigated by SEM was in coinciding with that by UV-Vis and FL experiments. The association equilibrium constant (K), the number of binding sites (n) and the standard free energy change (ΔG°) were calculated by using the Stern-Volmer method. These thermodynamic data indicated that the binding processes of Ag NPs and HSA are spontaneous, and the association complex can be formed in the solution.
- Ag nanoparticles,
- Human serum albumin,
- Spectroscopy,
- SEM,
- TEM
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