Citation: Su Xinyi, Liu Min, Yuan Mengjuan, Han Jun. Study on Thermal Stability of Human Serum Albumin at Different pH Values and Its Structural Reversibility[J]. Chemistry, ;2016, 79(4): 344-348. shu

Study on Thermal Stability of Human Serum Albumin at Different pH Values and Its Structural Reversibility

Su Xinyi ¹ ,
Liu Min ² ,
Yuan Mengjuan ¹ ,
Han Jun ^2,,

1.
1. School of Pharmacy, Liaocheng University, Liaocheng 252000;
2.
2. Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252000

Corresponding author: Han Jun,
Received Date: 4 September 2015
Available Online: 29 October 2015
Fund Project:

The tertiary structure of human serum albumin (HSA) and the structure-reversibility at different pH values had been studied by DSC, DLS and CD. The results indicated that HSA proves to be the most stable structure at pH 7.0,and the melting temperature (T_m) is 68.17±0.06℃. The tertiary structure of HSA is changed at different pH values and the T_m of HSA is very close to the T_m at pH 7.0 when pH is adjusted back to 7.0 from different pH values. The size of HSA is larger at other different pH values than that at pH 7.0 and it gets smaller when pH is adjusted back to 7.0. The influence of pH on the secondary structure of HSA is detected by CD. Although HSA can be refolded when adjusted back to 7.0 from different pH values, it can't return back to the structure of HSA at pH 7.0.
- DSC,
- Dynamic light scattering (DLS),
- Circular dichroism (CD),
- Partical size,
- Human serum albumin (HSA)
1. [1]
  [1] D C Carter, J X Ho. Adv. Protein Chem., 1994,45:153~203.
2. [2]
  [2] T Peters Jr. Adv. Protein Chem., 1985,37:161~245.
3. [3]
  [3] K Yamasaki, T Maruyama, U Kragh-Hansen et al.Biochim. Biophys. Acta, 1996,1295(2):147~157.
4. [4]
  [4] X M He, D C Carter. Nature, 1992, 358(6383):209~215.
5. [5]
  [5] A Michnik, K Michalik, Z Drzazga. Polish J. Environ. Stud., 2006, 15(4A):81~83.
6. [6]
  [6] A K Shaw, S K Pal. J. Photochem. Photobiol. B, 2008,90(1):69~77.
7. [7]
  [7] R Matsuda, J Anquizola, K S Hoy et al. Methods Mol. Biol., 2015,1286:255~277.
8. [8]
  [8] J G Mehtala, C Kulczar, M Lavan et al. Bioconjug. Chem., 2015,26(5):941~949.
9. [9]
  [9] Z D Zhivkova. Curr. Pharm. Des., 2015,21(14):1817~1830.
10. [10]
  [10] M Yamasaki, H Yano, K Aoki. Int. J. Biol. Macromol., 1990, 12(4):263~268.
11. [11]
  [11] B Farruggia, G A Picó. Int. J. Biol. Macromol., 1999,26(5):317~323.
12. [12]
  [12] B I Kurganov, A E Lyubarev, J M Sanchez-Ruiz et al. Biophys. Chem., 1997, 69(2~3):125~135.
13. [13]
  [13] P Tompa, P Bánki, M Bokor et al. Biophys. J., 2006,91(6):2243~2249.
14. [14]
  [14] C M Johnson. Arch. Biochem. Biophys., 2013, 531(1~2):100~109.
15. [15]
  [15] J Wen, K Arthur, L Chemmalil et al. J. Pharm. Sci., 2012, 101(3):955~964.
16. [16]
  [16] A L Kholodenko, J F Douglas. Phys. Rev. E Stat. Phys. Plasmas Fluids Relat. Interdiscip. Topics, 1995, 51(2):1081~1090.
17. [17]
  [17] J Panchal, J Kotarek, E Marszal et al. AAPS J., 2014,16(3):440~451.
1. [1]
  Haiyu Nie , Chenhui Zhang , Fengpei Du . Ideological and Political Design for the Preparation, Characterization and Particle Size Control Experiment of Nanoemulsion. University Chemistry, 2024, 39(2): 41-46. doi: 10.3866/PKU.DXHX202306055
2. [2]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
3. [3]
  Bingliang Li , Yuying Han , Dianyang Li , Dandan Liu , Wenbin Shang . One-Step Synthesis of Benorilate Guided by Green Chemistry Principles and in vivo Dynamic Evaluation. University Chemistry, 2024, 39(6): 342-349. doi: 10.3866/PKU.DXHX202311070
4. [4]
  Zihan Cheng , Kai Jiang , Jun Jiang , Henggang Wang , Hengwei Lin . Achieving thermal-stimulus-responsive dynamic afterglow from carbon dots by singlet-triplet energy gap engineering through covalent fixation. Acta Physico-Chimica Sinica, 2026, 42(2): 100169-0. doi: 10.1016/j.actphy.2025.100169
5. [5]
  Ziyu Bai , Jiaxin Zhou , Sisi Zhao , Siyu Teng , Xinxin Li , Xiting Wang , Yuwei Dong , Zhen Zhao . Delving into the Captivating Color Transformation of Cobalt: Digital Empowerment of Intelligent Visual Robot Monitoring System for Measuring Thermodynamic Parameters. University Chemistry, 2026, 41(1): 122-132. doi: 10.12461/PKU.DXHX202505103
6. [6]
  Tianrong Zhu , Fan Yu , Yuhang Liu , Haiyi Xu , Tingting Ma , Ming Li , Yuhang Xue , Yazhen Wang , Aihua Li , Biao Xiao , Xiaolun Peng . Intelligent Visualization, Precise Iodometry: Color Recognition-based Indirect Iodometric Method for Copper Determination. University Chemistry, 2026, 41(1): 264-275. doi: 10.12461/PKU.DXHX202503096
7. [7]
  Donghui PAN , Yuping XU , Xinyu WANG , Lizhen WANG , Junjie YAN , Dongjian SHI , Min YANG , Mingqing CHEN . Preparation and in vivo tracing of ⁶⁸Ga-labeled PM_2.5 mimetic particles for positron emission tomography imaging. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 669-676. doi: 10.11862/CJIC.20230468
8. [8]
  Zehua Zhao , Xiaoyan An , Jinrong Xu , Ling Yang , Hao Zhao , Zhongyun Wu . Independent Development and Application of Calorimetric Experiment Data Acquisition and Processing Software. University Chemistry, 2025, 40(11): 402-408. doi: 10.12461/PKU.DXHX202505045
9. [9]
  Wenkai Chen , Yunjia Shen , Xiangmeng Kong , Yanli Zeng . Quantum Chemistry Calculation of Key Physical Quantity in Circularly Polarized Luminescence: Introducing an Exploratory Computational Chemistry Experiment. University Chemistry, 2025, 40(3): 83-91. doi: 10.12461/PKU.DXHX202405018
10. [10]
  Lancanghong Chen , Xingtai Yu , Tianlei Zhao , Qizhi Yao . Exploration of Abnormal Phenomena in Iodometric Copper Quantitation Experiment. University Chemistry, 2025, 40(7): 315-320. doi: 10.12461/PKU.DXHX202408089
11. [11]
  Baohua LÜ , Yuzhen LI . Anisotropic photoresponse of two-dimensional layered α-In₂Se₃(2H) ferroelectric materials. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1911-1918. doi: 10.11862/CJIC.20240105
12. [12]
  Chengqian Mao , Yanghan Chen , Haotong Bai , Junru Huang , Junpeng Zhuang . Photodimerization of Styrylpyridinium Salt and Its Application in Silk Screen Printing. University Chemistry, 2024, 39(5): 354-362. doi: 10.3866/PKU.DXHX202312014
13. [13]
  Runhua Chen , Qiong Wu , Jingchen Luo , Xiaolong Zu , Shan Zhu , Yongfu Sun . Defective Ultrathin Two-Dimensional Materials for Photo-/Electrocatalytic CO₂ Reduction: Fundamentals and Perspectives. Acta Physico-Chimica Sinica, 2025, 41(3): 100019-0. doi: 10.3866/PKU.WHXB202308052
14. [14]
  Zhen Yao , Bing Lin , Youping Tian , Tao Li , Wenhui Zhang , Xiongwei Liu , Wude Yang . Visible-Light-Mediated One-Pot Synthesis of Secondary Amines and Mechanistic Exploration. University Chemistry, 2024, 39(5): 201-208. doi: 10.3866/PKU.DXHX202311033
15. [15]
  Jiaxin Su , Jiaqi Zhang , Shuming Chai , Yankun Wang , Sibo Wang , Yuanxing Fang . Optimizing Poly(heptazine imide) Photoanodes Using Binary Molten Salt Synthesis for Water Oxidation Reaction. Acta Physico-Chimica Sinica, 2024, 40(12): 2408012-0. doi: 10.3866/PKU.WHXB202408012
16. [16]
  Jing Kang , Tianyuan Zhao , Qian Zhang , Zhiwen Zhang , Yanyuan Jia , Alideertu Dong . Spotlight on Chlorine: An Innovative Iodometric Method for Visualizing Residual Chlorine. University Chemistry, 2026, 41(3): 343-350. doi: 10.12461/PKU.DXHX202506050
17. [17]
  Ruiqin Feng , Ye Fan , Yun Fang , Yongmei Xia . Strategy for Regulating Surface Protrusion of Gold Nanoflowers and Their Surface-Enhanced Raman Scattering. Acta Physico-Chimica Sinica, 2024, 40(4): 2304020-0. doi: 10.3866/PKU.WHXB202304020
18. [18]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
19. [19]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
20. [20]
  Ting Li , Xiao Zeng , Yuzhuo Yang , Xinyi Wen , Shurong Ding , Linlin Shi , Yongqiang Zhang , Siyu Lu . Towards practical circularly polarized luminescence: carbon dots-based circularly polarized lasers. Acta Physico-Chimica Sinica, 2026, 42(4): 100191-0. doi: 10.1016/j.actphy.2025.100191

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1274)
HTML views(241)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142