引用本文:
李润, 张万宽, 倪杭生, 戴俊彪, 王宏, 周春玉. 金藻Isochrysis Galbana中DHA的分离与分析[J]. 应用化学,
2001, 18(6): 454-456.
Citation: LI Run, ZHANG Wan-Kuan, NI Hang-Sheng, DAI Jun-Biao, WANG Hong, ZHOU Chun-Yu. Separation and Analysis DHA from Isochrysis Galbana[J]. Chinese Journal of Applied Chemistry, 2001, 18(6): 454-456.
Citation: LI Run, ZHANG Wan-Kuan, NI Hang-Sheng, DAI Jun-Biao, WANG Hong, ZHOU Chun-Yu. Separation and Analysis DHA from Isochrysis Galbana[J]. Chinese Journal of Applied Chemistry, 2001, 18(6): 454-456.
金藻Isochrysis Galbana中DHA的分离与分析
English
Separation and Analysis DHA from Isochrysis Galbana
Abstract:
Isochrysis galbana is used as a new source of DHA (docosahexaenoic acid). The fatty acid of Isochrysis galbana was extracted by a mixture of CH3Cl, CH3OH and H2O(1:2:0.8 in volume) and esterified by a mixture of BF3 and C2H5OH(1:3 in volume), respectively. The esterification degree was detected with silica gel thin films. The quantitative analysis for DHA was carried out by GC and GC/MS techniques using ethyl ester of C19 fatty acid as an internal standard. DHA in yield of 2.2% was obtained from algae isochrysis galbana.
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Key words:
- isochrysis galbana
- / docosahexaenoic acid
- / separation
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