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Citation: XIA Hai-Tao, LIU Yu-Fen, CHEN Hong-Wei. Analysis of Soybean Oilgosaccharides by HPLC with ELS Detector[J]. Chinese Journal of Applied Chemistry, ;2006, 23(4): 462-464. shu

Analysis of Soybean Oilgosaccharides by HPLC with ELS Detector

1.
a Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005;
2.
b College of Bioengineering, Changchun University of Technology, Changchun

Corresponding author: XIA Hai-Tao,
Received Date: 3 July 2005
Available Online: 29 August 2005

Soybean oligosaccharides were analyzed via high performance liquid chromatography with gradient elution of acetonitril-water and an evaporative light-scattering detector.The three important components, sucrose,raffinose,stachyose of soybean oilgosaccharides were separated completely from other components within twenty minutes.The quantitative analysis results of the three components were achieved with the external standard method,and the linear relations were good.The recovery of this method is 96.5%~99.1%,the relative standard deviations are in a range of 0.18%~4.67%.Operation of the method is simple and fast,applicable to the routine analysis of industrial products.
- HPLC,
- ELS detector,
- gradient elution,
- soybean,
- oligosaccharide
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