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Citation: ZHANG Ming-Cui, ZHUANG Hui-Sheng, LANG Qing. Immunological Detection of Dibutyl Phthalate Ⅰ. Preparation and Characterization of Artificial Antigen[J]. Chinese Journal of Applied Chemistry, ;2006, 23(8): 850-853. shu

Immunological Detection of Dibutyl Phthalate Ⅰ. Preparation and Characterization of Artificial Antigen

1.
a College of Environmental Science and Engineering, Donghua University, Shanghai 200051;
2.
b College of Chemistry and materials Science, Anhui Normal University, Wuhu

Corresponding author: ZHUANG Hui-Sheng,
Received Date: 3 September 2005
Available Online: 10 November 2005
Fund Project: 国家自然科学基金资助项目(20277005) (20277005)

Dibutyl phthalate hapten(dibutyl 4-aminophthalate) was synthesized by introducing amino group as a substituent at the aromatic ring while retaining the ester group.The product was characterized by means of ¹H NMR,IR and UV,with λ₁=212 nm,λ₂=258 nm for the UV of dibutyl 4-nitrophthalate and λ₁=226 nm,λ₂=288 nm for the UV of dibutyl 4-aminophthalate.The hapten was conjugated to BSA via amino diazotization linkage.Thus artificial antigen was prepared and tested by fluorescence spectrum with λ_ex=307 nm,λ_em=468 nm,and the approximate molar ratio of dibutyl 4-aminophthalate to BSA was 13.The product was used as an immunogen,demonstrating that it is suitable for polyclonal antibody production.The present research has supplied an excellent immune antigen for further preparation and immunoassay of antibody obtained from dibutyl phthalate.
- dibutyl phthalate,
- artificial antigen,
- hapten,
- preparation,
- characterization
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