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Citation: XING Fei-Fei, SONG Guang-Tao, REN Jin-Song. RNA Triple-Helix Recognition and Stabilization by Coralyne[J]. Chinese Journal of Applied Chemistry, ;2006, 23(12): 1404-1406. shu

RNA Triple-Helix Recognition and Stabilization by Coralyne

1.
Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Corresponding author: REN Jin-Song,
Received Date: 24 December 2005
Available Online: 8 March 2006
Fund Project: 国家杰出青年基金(20325101)资助项目 (20325101)

Small molecule coralyne was found to bind preferentially and strongly to RNA in triple helical state.The binding was characterized by means of circular dichroism,fluorescence spectroscopy,thermal denaturation and competition dialysis assay.The binding constant(K_app) is (2.0±0.4)×10⁶(mol/L)^-1,and the melting temperature of polyA(polyU)₂ can be increased by coralyne for about 11℃.
- RNA triple helix,
- anticancer drug,
- stabilization,
- coralyne,
- recognition
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