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Citation: CAI Huai-Hong, YANG Pei-Hui, CAI Ji-Ye. Electrochemical Study on Interaction Between Artemisinin and DNA[J]. Chinese Journal of Applied Chemistry, ;2007, 24(4): 411-415. shu

Electrochemical Study on Interaction Between Artemisinin and DNA

1.
Department of Chemistry, Ji'nan University, Guangzhou 510632

Corresponding author: YANG Pei-Hui,
Received Date: 20 May 2006
Available Online: 11 October 2006
Fund Project: 广东省自然科学基金(021190) (021190)广州市科技计划项目基金(2003Z-D2041) (2003Z-D2041)

The interaction between artemisinin and DNA was studied by cyclic voltammetry and UV-Vis spectroscopy in a Britton-Robinson buffer solution(pH=7.4) containing 20% ethanol.The results would be helpful to understanding the antitumor mechanism of artemisinin and its derivatives.The electrochemical results show that the reduction peak current of artemisinin at a potential of -0.672 V at sliver electrode decreased and the peak potential shifted positively with adding DNA in artemisinin solution.The comparison of the interactions of dsDNA and ssDNA with artemisinin indicates that artemisinin reacted with DNA in a mode of intercalation to form an electrochemically inactive complex.The calculation via electrochemical method shows that the ratio of DNA:artemisinin was 1:4 and the combination constant was 3.6×10⁴.The determination of electrochemical parameters(α,β and K_s) in the absence and the presence of DNA also shows that an electrochemically inactive complex was formed and the reaction was controlled by a diffusion-controlled electrode process.UV-Vis spectroscopic studies further confirmed the intercalation between artemisinin and DNA.The experiments of spectroscopic titration indicated that one DNA molecule combined with four artemisinin molecules and the binding constant reached 4.0×10⁴,which was similar to the result from electrochemical method.These results show that artemisinin might combine with the DNA of nucleus which induced the apoptosis of the cells when it entered into them.
- artemisinin,
- DNA,
- cyclic voltammetry,
- UV-Vis spectroscopy
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