引用本文:
杨朝霞, 张友玉, 杨琴, 姚守拙. 光谱法研究呋苄西林钠与牛血清白蛋白的作用[J]. 应用化学,
2007, 24(8): 858-862.
Citation: YANG Zhao-Xia, ZHANG You-Yu, YANG Qin, YAO Shou-Zhuo. Spectroscopic Study on the Interaction of Furbenicillin Sodium with Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2007, 24(8): 858-862.
Citation: YANG Zhao-Xia, ZHANG You-Yu, YANG Qin, YAO Shou-Zhuo. Spectroscopic Study on the Interaction of Furbenicillin Sodium with Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2007, 24(8): 858-862.
光谱法研究呋苄西林钠与牛血清白蛋白的作用
English
Spectroscopic Study on the Interaction of Furbenicillin Sodium with Bovine Serum Albumin
Abstract:
Bonding reaction of Furbenicillin Sodium (FBS) with bovine serum albumin (BSA) was studied by means of UV-Vis,fluorescence and infrared spectroscopy.The experimental results suggest that there is strong interaction between FBS and BSA,and FBS has a strong quenching effect on the BSA fluorescence.It is confirmed from fluorescence data that the quenching effect of FBS on the BSA fluorescence is a single static quenching process.The fluorescence quenching data was analyzed using the Stern-Volmer equation and double-logarithm equation.The binding constant and binding sites were 1.04×105 and 1.09 at ambient temperature (26℃),respectively.It is revealed from the above results that the bonding of FBS with BSA leads to a significant change of the secondary structure of BSA.
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