Citation: Dong Gao, Fu-Chun Tan, Wen-Peng Wang, Li-Li Wang. Resolution enhancement in hydrophobic interaction chromatography via electrostatic interactions[J]. Chinese Chemical Letters, ;2013, 24(05): 419-421. shu

Resolution enhancement in hydrophobic interaction chromatography via electrostatic interactions

1.
Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, Shaanxi Key Laboratory of Modern Separation Science, Institute of Modern Separation Science, Northwest University, Xi'an 710069, China

Corresponding author: Dong Gao,
Received Date: 10 December 2012
Available Online: 1 February 2013

In this work, a new type of hydrophobic stationary phase that provide electrostatic interactions with analytes was developed by bonding β-phenylethylamine as a functional ligand to silica. This stationary phase can separate proteins with similar hydrophobicity that traditional hydrophobic resins cannot. Hen egg white was separated to examine the selectivity. The results show that the introduced electrostatic interactions are an important factor for the resolution enhancement and the new resin could have important applications in separation and purification of biological macromolecules.
- Hydrophobic interaction chromatography,
- Electrostatic interaction,
- Resolution,
- Hen egg white
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沈阳化工大学材料科学与工程学院沈阳 110142