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Citation: Li Hua CHEN. Improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip[J]. Chinese Chemical Letters, ;2006, 17(12): 1619-1622. shu

Improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip

Li Hua CHEN

College of chemistry and molecular engineer, Qingdao University of Science and Technology, Qingdao 266042

Received Date: 1 September 2006

Fund Project: This work was supported by Qingdao University of Science and Technology. The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.

A chip was modified with bovine serum albumin (BSA), then interaction between glutathione (GSH) immobilized on the top of BSA and glutathione-S-transferase (GST) was examined, using surface plasmon resonance (SPR). The SPR results showed that BSA-modified chip was effective not only in binding the target proteins but also in suppressing the nonspecific binding (NSB) of proteins.
- Surface plasmon resonance (SPR),
- bovine serum albumin (BSA),
- glutathione (GSH),
- glutathione-S-transferase (GST)
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