{"聚焦":[{"abstractInfo":"","abstractInfoCn":"","abstractInfoEn":"","articleNo":"sp1603020","authorCnList":["龙星宇"," 练鸿振"],"authorEnList":[],"authorList":["龙星宇"," 练鸿振"],"authors":"龙星宇, 练鸿振","authorsCn":"龙星宇, 练鸿振","authorsEn":"","categoryName":"聚焦","categoryNameCn":"聚焦","categoryNameEn":"","citation":"龙星宇, 练鸿振. 基于质谱技术的磷酸化蛋白/多肽分离富集、鉴定及组学研究. 色谱, 2016, 34(4): 357-359. doi: 10.3724/SP.J.1123.2016.03020.","citationCn":"龙星宇, 练鸿振. 基于质谱技术的磷酸化蛋白/多肽分离富集、鉴定及组学研究. 色谱, 2016, 34(4): 357-359. doi: 10.3724/SP.J.1123.2016.03020.","citationEn":"龙星宇, 练鸿振. . Chinese Journal of Chromatography, 2016, 34(4): 357-359. doi: 10.3724/SP.J.1123.2016.03020.","doi":"10.3724/SP.J.1123.2016.03020","figContent":"","figList":[],"firstFig":"","fpage":"357","highCitedState":"0","htmlCount":14,"htmlFile":"","id":"338","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"","keywordCn":"","keywordCnList":[],"keywordEn":"","keywordEnList":[],"keywordList":[],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"359","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":748,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"基于质谱技术的磷酸化蛋白/多肽分离富集、鉴定及组学研究","titleCn":"基于质谱技术的磷酸化蛋白/多肽分离富集、鉴定及组学研究","titleEn":"","viewCount":401,"volume":"34","year":2016}],"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏":[{"abstractInfo":"核酸适配体特指具有分子识别功能的短序列的单链核酸,也被称为\"化学抗体\"。它既有可与抗体相当的亲和力和特异性,又有明显优于抗体的特点:可通过化学合成制备,无需动物免疫,成本低;分子性质稳定,能可逆变性与复性,易储存运输;分子量小,易穿过细胞膜,免疫原性低,无明显的毒副作用;靶标范围广泛,包括金属离子、小分子、蛋白质、细胞和微生物等。WebofScience数据库显示,仅2010年以来就有超过5300篇核酸适配体论文发表,涉及生物医学、分子生物学、化学生物学、分析化学、材料化学、物理学、数学等学科领域。核酸适配体研究具有交叉学科特色,与核酸适配体应用相关的食品、环境和生物分析检测,以及核酸适配体在疾病诊断和治疗、药物研发和分子成像领域的应用潜力正吸引多学科领域研究者的广泛关注。我国学者在核酸适配体研究领域取得大量处于世界前列的研究成果。2009年以来,约300个核酸适配体相关项目获国家重大研究计划以及国家自然科学基金资助。近3年来,在生物、医学、中医药、环境和食品安全检测领域的核酸适配体相关应用研究也在快速增加。<br>纵观核酸适配体25年的发展,已报道的靶标超过500种,筛选出的核酸适配体超过2300种。但大量的研究和应用主要集中于一些常见的靶标和特定的核酸适配体。令人满意的具有实际应用价值的核酸适配体数目还非常有限。研究者普遍的共识是,核酸适配体的筛选过程复杂,筛选效率低,且缺少标准化的方法表征适配体的功能,这是目前制约核酸适配体广泛应用的两个瓶颈。","abstractInfoCn":"核酸适配体特指具有分子识别功能的短序列的单链核酸,也被称为\"化学抗体\"。它既有可与抗体相当的亲和力和特异性,又有明显优于抗体的特点:可通过化学合成制备,无需动物免疫,成本低;分子性质稳定,能可逆变性与复性,易储存运输;分子量小,易穿过细胞膜,免疫原性低,无明显的毒副作用;靶标范围广泛,包括金属离子、小分子、蛋白质、细胞和微生物等。Web of Science数据库显示,仅2010年以来就有超过5300篇核酸适配体论文发表,涉及生物医学、分子生物学、化学生物学、分析化学、材料化学、物理学、数学等学科领域。核酸适配体研究具有交叉学科特色,与核酸适配体应用相关的食品、环境和生物分析检测,以及核酸适配体在疾病诊断和治疗、药物研发和分子成像领域的应用潜力正吸引多学科领域研究者的广泛关注。我国学者在核酸适配体研究领域取得大量处于世界前列的研究成果。2009年以来,约300个核酸适配体相关项目获国家重大研究计划以及国家自然科学基金资助。近3年来,在生物、医学、中医药、环境和食品安全检测领域的核酸适配体相关应用研究也在快速增加。<br>纵观核酸适配体25年的发展,已报道的靶标超过500种,筛选出的核酸适配体超过2300种。但大量的研究和应用主要集中于一些常见的靶标和特定的核酸适配体。令人满意的具有实际应用价值的核酸适配体数目还非常有限。研究者普遍的共识是,核酸适配体的筛选过程复杂,筛选效率低,且缺少标准化的方法表征适配体的功能,这是目前制约核酸适配体广泛应用的两个瓶颈。","abstractInfoEn":"","articleNo":"sp1602003","authorCnList":["屈锋"],"authorEnList":[],"authorList":["屈锋"],"authors":"屈锋","authorsCn":"屈锋","authorsEn":"","categoryName":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameCn":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameEn":"","citation":"屈锋. 引言. 色谱, 2016, 34(4): 360-360. doi: 10.3724/SP.J.1123.2016.02003.","citationCn":"屈锋. 引言. 色谱, 2016, 34(4): 360-360. doi: 10.3724/SP.J.1123.2016.02003.","citationEn":"屈锋. . Chinese Journal of Chromatography, 2016, 34(4): 360-360. doi: 10.3724/SP.J.1123.2016.02003.","doi":"10.3724/SP.J.1123.2016.02003","figContent":"","figList":[],"firstFig":"","fpage":"360","highCitedState":"0","htmlCount":49,"htmlFile":"","id":"339","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"","keywordCn":"","keywordCnList":[],"keywordEn":"","keywordEnList":[],"keywordList":[],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"360","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":524,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"引言","titleCn":"引言","titleEn":"","viewCount":490,"volume":"34","year":2016},{"abstractInfo":"核酸适配体(aptamer)是通过指数富集配体系统进化(SELEX)技术筛选得到的核糖核酸(RNA)或单链脱氧核糖核酸(ssDNA)。核酸适配体通过高亲和力特异性地识别小分子、蛋白质、细胞、微生物等多种靶标,在生物、医药、食品和环境检测等领域的应用日渐增多。但目前实际可用的核酸适配体有限,其筛选过程复杂,筛选难度大,制约了其应用。与生物大分子、细胞和微生物等靶标不同,小分子靶标与核酸分子的结合位点少、亲和力弱,且靶标通常需要固定在载体上。此外,小分子靶标结合核酸形成的复合物与核酸自身的大小、质量、电荷性质等方面差异较小,二者的分离难度大。故小分子靶标的核酸适配体筛选过程与大分子和细胞等复合靶标相比有明显差异,筛选难度更大。因此需要根据其自身结构特点和核酸适配体的应用目的选定靶标或核酸库的固定方法,优化靶标核酸复合物的分离方法。本文介绍了不同类型小分子(具有基团差异的单分子、含相同基团分子和手性分子等)靶标的选择及其核酸适配体的筛选方法,并对核酸库的设计、与靶标结合的核酸的分离方法和亲和作用表征方法进行了介绍,列出了自2008年以来报道的40余种小分子靶标的核酸适配体序列和复合物的平衡解离常数(<i>K<\/i><sub>d<\/sub>)。","abstractInfoCn":"核酸适配体(aptamer)是通过指数富集配体系统进化(SELEX)技术筛选得到的核糖核酸(RNA)或单链脱氧核糖核酸(ssDNA)。核酸适配体通过高亲和力特异性地识别小分子、蛋白质、细胞、微生物等多种靶标,在生物、医药、食品和环境检测等领域的应用日渐增多。但目前实际可用的核酸适配体有限,其筛选过程复杂,筛选难度大,制约了其应用。与生物大分子、细胞和微生物等靶标不同,小分子靶标与核酸分子的结合位点少、亲和力弱,且靶标通常需要固定在载体上。此外,小分子靶标结合核酸形成的复合物与核酸自身的大小、质量、电荷性质等方面差异较小,二者的分离难度大。故小分子靶标的核酸适配体筛选过程与大分子和细胞等复合靶标相比有明显差异,筛选难度更大。因此需要根据其自身结构特点和核酸适配体的应用目的选定靶标或核酸库的固定方法,优化靶标核酸复合物的分离方法。本文介绍了不同类型小分子(具有基团差异的单分子、含相同基团分子和手性分子等)靶标的选择及其核酸适配体的筛选方法,并对核酸库的设计、与靶标结合的核酸的分离方法和亲和作用表征方法进行了介绍,列出了自2008年以来报道的40余种小分子靶标的核酸适配体序列和复合物的平衡解离常数(<i>K<\/i><sub>d<\/sub>)。","abstractInfoEn":"Aptamers are ribonucleic acid (RNA) or single-stranded deoxyribonucleic acid (ssDNA) selected by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers can identify small molecules, proteins, cells, microorganisms and other targets with high affinity and specificity, and have been widely applied in biology, medicine, food and environmental monitoring. However, available aptamers of practical use are limited. The complex and difficult screening of aptamers are the key to restrict its wide application. Differing from biomacromolecules, cells and microorganisms, small molecules have less binding sites and weaker affinity with nucleic acids. And they usually need to be immobilized on substrates. In addition, due to the tiny differences of size, weight and charge of the target-ssDNA/RNA complex and ssDNA/RNA, their separation is difficult. Therefore, the aptamer selection of small molecules is more difficult than biomacromolecules or cells. The selection of methods for immobilizing the targets or library and the optimization of separation process proceed mainly based on the structure characteristics and applications of aptamers. In this paper, the screening methods for molecules with different groups, molecules containing the same group and chiral molecules are introduced. Also, the library design, the methods for separating targets-ssDNA complex and characterizing affinity interaction are discussed. The sequences and dissociation constants (<i>K<\/i><sub>d<\/sub>) of about 40 aptamers reported since 2008 are listed.","articleNo":"sp1505001","authorCnList":["王勇"," 赵新颖"," 石冬冬"," 杨歌"," 屈锋"],"authorEnList":["WANG  Yong"," ZHAO  Xinying"," SHI  Dongdong"," YANG  Ge"," QU  Feng"],"authorList":["王勇"," 赵新颖"," 石冬冬"," 杨歌"," 屈锋"],"authors":"王勇, 赵新颖, 石冬冬, 杨歌, 屈锋","authorsCn":"王勇, 赵新颖, 石冬冬, 杨歌, 屈锋","authorsEn":"WANG  Yong, ZHAO  Xinying, SHI  Dongdong, YANG  Ge, QU  Feng","categoryName":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameCn":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameEn":"","citation":"王勇, 赵新颖, 石冬冬, 杨歌, 屈锋. 小分子靶标的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 361-369. doi: 10.3724/SP.J.1123.2015.05001.","citationCn":"王勇, 赵新颖, 石冬冬, 杨歌, 屈锋. 小分子靶标的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 361-369. doi: 10.3724/SP.J.1123.2015.05001.","citationEn":"王勇, 赵新颖, 石冬冬, 杨歌, 屈锋. Research advances of aptamers selection for small molecule targets. Chinese Journal of Chromatography, 2016, 34(4): 361-369. doi: 10.3724/SP.J.1123.2015.05001.","doi":"10.3724/SP.J.1123.2015.05001","figContent":"","figList":[],"firstFig":"","fpage":"361","highCitedState":"0","htmlCount":176,"htmlFile":"","id":"340","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"核酸适配体, 小分子靶标, 筛选, 综述","keywordCn":"核酸适配体, 小分子靶标, 筛选, 综述","keywordCnList":["核酸适配体"," 小分子靶标"," 筛选"," 综述"],"keywordEn":"aptamer, small molecule targets, screening, review","keywordEnList":["aptamer"," small molecule targets"," screening"," review"],"keywordList":["核酸适配体"," 小分子靶标"," 筛选"," 综述"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"369","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":5,"pdfSize":889,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"小分子靶标的核酸适配体筛选的研究进展","titleCn":"小分子靶标的核酸适配体筛选的研究进展","titleEn":"Research advances of aptamers selection for small molecule targets","viewCount":896,"volume":"34","year":2016},{"abstractInfo":"核酸适配体是通过指数富集系统配体进化(SELEX)筛选获得的,与靶标具有高亲和力和特异性结合的单链DNA或RNA。蛋白质是生命进程中的关键功能分子。近年来,以蛋白质为靶标的适配体筛选在蛋白质相关的基础及应用研究领域受到广泛关注。核酸适配体应用性能的优劣取决于其亲和力、特异性与稳定性。目前,适配体筛选方法的优化主要是提高筛选效率、提升适配体性能及降低筛选成本。适配体主要筛选步骤包括复合物分离、核酸库优化、次级库的富集、适配体序列分析以及亲和力表征等。迄今为止,以蛋白质-核酸复合物的分离为核心步骤的适配体筛选方法有20余种。本文归纳总结了2005年以来以蛋白质为靶标的适配体筛选技术,讨论了各方法的缺陷与局限。介绍了核酸库的设计优化方法、适配体的序列特征,以及常用的亲和力表征方法。","abstractInfoCn":"核酸适配体是通过指数富集系统配体进化(SELEX)筛选获得的,与靶标具有高亲和力和特异性结合的单链DNA或RNA。蛋白质是生命进程中的关键功能分子。近年来,以蛋白质为靶标的适配体筛选在蛋白质相关的基础及应用研究领域受到广泛关注。核酸适配体应用性能的优劣取决于其亲和力、特异性与稳定性。目前,适配体筛选方法的优化主要是提高筛选效率、提升适配体性能及降低筛选成本。适配体主要筛选步骤包括复合物分离、核酸库优化、次级库的富集、适配体序列分析以及亲和力表征等。迄今为止,以蛋白质-核酸复合物的分离为核心步骤的适配体筛选方法有20余种。本文归纳总结了2005年以来以蛋白质为靶标的适配体筛选技术,讨论了各方法的缺陷与局限。介绍了核酸库的设计优化方法、适配体的序列特征,以及常用的亲和力表征方法。","abstractInfoEn":"Aptamers are selected through systematic evolution of ligands by exponential enrichment (SELEX), and are artificially synthesized single-stranded DNA or RNA with high affinity and specificity against a wide variety of target molecules. Since some functional proteins play the key role in life process, aptamers against proteins have attracted great attention in the last decade, and are used in basic research and wide applications. The performance of aptamers depends on their affinity, specificity and stability. In recent years, many SELEX methods have been developed to enhance the properties of aptamers, improve selection efficiency and reduce cost. The main procedure of SELEX includes the isolation of target-aptamer compound, optimization the random ssDNA library, enrichment of ssDNA and the analysis and characterization of selected aptamers. In this review, we summarize the developments of aptamer selection methods for protein targets since 2005, discuss their shortcomings and limitations, and introduce the optimization of ssDNA library, aptamer sequence character and analytical methods for their affinity analysis.","articleNo":"sp1512014","authorCnList":["杨歌"," 魏强"," 赵新颖"," 屈锋"],"authorEnList":["YANG  Ge"," WEI  Qiang"," ZHAO  Xinying"," QU  Feng"],"authorList":["杨歌"," 魏强"," 赵新颖"," 屈锋"],"authors":"杨歌, 魏强, 赵新颖, 屈锋","authorsCn":"杨歌, 魏强, 赵新颖, 屈锋","authorsEn":"YANG  Ge, WEI  Qiang, ZHAO  Xinying, QU  Feng","categoryName":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameCn":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameEn":"","citation":"杨歌, 魏强, 赵新颖, 屈锋. 蛋白质的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 370-381. doi: 10.3724/SP.J.1123.2015.12014.","citationCn":"杨歌, 魏强, 赵新颖, 屈锋. 蛋白质的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 370-381. doi: 10.3724/SP.J.1123.2015.12014.","citationEn":"杨歌, 魏强, 赵新颖, 屈锋. Research advances of aptamers selection for protein targets. Chinese Journal of Chromatography, 2016, 34(4): 370-381. doi: 10.3724/SP.J.1123.2015.12014.","doi":"10.3724/SP.J.1123.2015.12014","figContent":"","figList":[],"firstFig":"","fpage":"370","highCitedState":"0","htmlCount":88,"htmlFile":"","id":"341","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"核酸适配体, 蛋白质, 筛选, 指数富集系统配体进化, 综述","keywordCn":"核酸适配体, 蛋白质, 筛选, 指数富集系统配体进化, 综述","keywordCnList":["核酸适配体"," 蛋白质"," 筛选"," 指数富集系统配体进化"," 综述"],"keywordEn":"aptamer, protein, selection, systematic evolution of ligands by exponential enrichment (SELEX), review","keywordEnList":["aptamer"," protein"," selection"," systematic evolution of ligands by exponential enrichment (SELEX)"," review"],"keywordList":["核酸适配体"," 蛋白质"," 筛选"," 指数富集系统配体进化"," 综述"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"381","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":926,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"蛋白质的核酸适配体筛选的研究进展","titleCn":"蛋白质的核酸适配体筛选的研究进展","titleEn":"Research advances of aptamers selection for protein targets","viewCount":854,"volume":"34","year":2016},{"abstractInfo":"核酸适配体(aptamer)是从人工合成的随机单链DNA(ssDNA)或RNA文库中筛选得到的,能够高亲和力、高特异性地与靶标结合的ssDNA或RNA。核酸适配体的靶标范围广,可包括小分子、蛋白质、细胞、微生物等多种靶标。其中以细胞为靶标的适配体在生物感应、分子成像、医学诊断、药物传输和疾病治疗等领域有很大的应用潜能。但全细胞的核酸适配体筛选过程复杂,筛选难度大,筛选的适配体性能不佳是导致目前可用的适配体非常有限的主要原因。由于细胞表面蛋白质在提取纯化过程中分子结构和形态会发生改变,故以膜表面蛋白质为靶标筛选的适配体很难应用于识别整体细胞。以全细胞为靶标的核酸适配体筛选则不需要准确了解细胞表面的分子结构,筛选过程中可保持细胞的天然状态,以全细胞为靶标筛选出的核酸适配体有望直接用于全细胞识别。本文总结了2008~2015年全细胞的核酸适配体筛选的研究进展,介绍了靶细胞的分类、核酸库的设计、筛选条件和方法以及核酸适配体的亲和力表征方法等。并列出全细胞靶标的核酸适配体序列。","abstractInfoCn":"核酸适配体(aptamer)是从人工合成的随机单链DNA(ssDNA)或RNA文库中筛选得到的,能够高亲和力、高特异性地与靶标结合的ssDNA或RNA。核酸适配体的靶标范围广,可包括小分子、蛋白质、细胞、微生物等多种靶标。其中以细胞为靶标的适配体在生物感应、分子成像、医学诊断、药物传输和疾病治疗等领域有很大的应用潜能。但全细胞的核酸适配体筛选过程复杂,筛选难度大,筛选的适配体性能不佳是导致目前可用的适配体非常有限的主要原因。由于细胞表面蛋白质在提取纯化过程中分子结构和形态会发生改变,故以膜表面蛋白质为靶标筛选的适配体很难应用于识别整体细胞。以全细胞为靶标的核酸适配体筛选则不需要准确了解细胞表面的分子结构,筛选过程中可保持细胞的天然状态,以全细胞为靶标筛选出的核酸适配体有望直接用于全细胞识别。本文总结了2008~2015年全细胞的核酸适配体筛选的研究进展,介绍了靶细胞的分类、核酸库的设计、筛选条件和方法以及核酸适配体的亲和力表征方法等。并列出全细胞靶标的核酸适配体序列。","abstractInfoEn":"Aptamers are single stranded DNA (ssDNA) or RNA that may bind to small molecules, proteins, cells, microorganisms, and other targets. Typically, aptamers are generated by a selection process referred to as systematic evolution of ligands by exponential enrichment (SELEX). Aptamers that bind with high affinity and specificity to proteins that reside on the cell surface have potential utility in the fields of biosenser, molecular imaging, medical diagnosis, drug delivery, and disease treatment. However, till now, available aptamers are very limited mainly due to the complex and difficulty screening of aptamer and their poor properties. Using purified cell surface proteins as target has the disadvantage of changing structure. When target proteins are present in a modified state, the isolated aptamers might not recognize the natural structure of some proteins. Aptamers screening targeting whole cell has the potential to be used as cell probe for directing whole cell analysis. It does not need to understand the molecular structure of the cell surface protein and maintain the natural state of cells in the screening process. This review mainly focuses on the advances of aptamer selection for whole cell from 2008 to 2015. The contents include the classification of target cells, design of library, methods for cell-ssDNA complex separation and affinity characterization. The sequences of aptamers reported are listed.","articleNo":"sp1512015","authorCnList":["刘品多"," 屈锋"],"authorEnList":["LIU  Pinduo"," QU  Feng"],"authorList":["刘品多"," 屈锋"],"authors":"刘品多, 屈锋","authorsCn":"刘品多, 屈锋","authorsEn":"LIU  Pinduo, QU  Feng","categoryName":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameCn":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameEn":"","citation":"刘品多, 屈锋. 全细胞的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 382-388. doi: 10.3724/SP.J.1123.2015.12015.","citationCn":"刘品多, 屈锋. 全细胞的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 382-388. doi: 10.3724/SP.J.1123.2015.12015.","citationEn":"刘品多, 屈锋. Research advances of aptamer selection for whole cell. Chinese Journal of Chromatography, 2016, 34(4): 382-388. doi: 10.3724/SP.J.1123.2015.12015.","doi":"10.3724/SP.J.1123.2015.12015","figContent":"","figList":[],"firstFig":"","fpage":"382","highCitedState":"0","htmlCount":38,"htmlFile":"","id":"342","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"核酸适配体, 细胞, 筛选, 指数富集的配基系统进化技术, 综述","keywordCn":"核酸适配体, 细胞, 筛选, 指数富集的配基系统进化技术, 综述","keywordCnList":["核酸适配体"," 细胞"," 筛选"," 指数富集的配基系统进化技术"," 综述"],"keywordEn":"aptamers, cells, selection, systematic evolution of ligands by exponential enrichment (SELEX), review","keywordEnList":["aptamers"," cells"," selection"," systematic evolution of ligands by exponential enrichment (SELEX)"," review"],"keywordList":["核酸适配体"," 细胞"," 筛选"," 指数富集的配基系统进化技术"," 综述"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"388","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":837,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"全细胞的核酸适配体筛选的研究进展","titleCn":"全细胞的核酸适配体筛选的研究进展","titleEn":"Research advances of aptamer selection for whole cell","viewCount":458,"volume":"34","year":2016},{"abstractInfo":"核酸适配体(aptamer)是通过指数富集配体系统进化技术(SELEX)筛选的能够以高亲和力和高特异性识别靶标分子或细胞的核糖核酸(RNA)和单链脱氧核糖核酸(ssDNA)。作为化学抗体,核酸适配体的制备和合成比抗体的成本更低。核酸适配体的靶标范围极其广泛,包括小分子、生物大分子、细菌和细胞等。针对细菌靶标筛选的适配体,目前主要应用于食品、医药和环境中的细菌检测。细菌的核酸适配体筛选可以通过离心法将菌体-适配体复合物与游离的适配体分离,并通过荧光成像、荧光光谱分析、流式细胞仪分选、DNA捕获元件、酶联适配体分析等方法表征适配体与靶标的相互作用。筛选出的适配体可结合生物、化学检测方法用于细菌检测。本文介绍了细菌适配体的筛选和表征方法以及基于适配体的检测方法的最新进展,分析了不同检测方法的利弊,并列出了2011~2015年筛选的细菌的核酸适配体。","abstractInfoCn":"核酸适配体(aptamer)是通过指数富集配体系统进化技术(SELEX)筛选的能够以高亲和力和高特异性识别靶标分子或细胞的核糖核酸(RNA)和单链脱氧核糖核酸(ssDNA)。作为化学抗体,核酸适配体的制备和合成比抗体的成本更低。核酸适配体的靶标范围极其广泛,包括小分子、生物大分子、细菌和细胞等。针对细菌靶标筛选的适配体,目前主要应用于食品、医药和环境中的细菌检测。细菌的核酸适配体筛选可以通过离心法将菌体-适配体复合物与游离的适配体分离,并通过荧光成像、荧光光谱分析、流式细胞仪分选、DNA捕获元件、酶联适配体分析等方法表征适配体与靶标的相互作用。筛选出的适配体可结合生物、化学检测方法用于细菌检测。本文介绍了细菌适配体的筛选和表征方法以及基于适配体的检测方法的最新进展,分析了不同检测方法的利弊,并列出了2011~2015年筛选的细菌的核酸适配体。","abstractInfoEn":"Aptamers are RNA or singal stranded DNA (ssDNA), which are selected by systematic evolution of ligands by exponential enrichment (SELEX). Compared to antibodies, aptamers as chemical antibodies are artificially synthesized with low cost. They can bind a wide range of targets, including small molecules, large biological molecules, bacteria and cells. Aptamers targeting bacteria have the potential to be used for pathogen detection in food, medicine and environment. To select aptamers with high affinity to bacteria, centrifugation is mostly used. Binding affinity can be estimated by fluorescence imaging, fluorescence spectra, flow cytometry, DNA capture element (DCE) and enzyme-linked aptamers sorbent assay (ELASA). Selected aptamers combined with biological and chemical analysis methods can be used in bacteria detection. This article introduces the latest development in aptamer selection, characterization and application for bacteria detection and summarizes the aptamers for bacteria from 2011 to 2015.","articleNo":"sp1512021","authorCnList":["陈尔凝"," 赵新颖"," 屈锋"],"authorEnList":["CHEN  Erning"," ZHAO  Xinying"," QU  Feng"],"authorList":["陈尔凝"," 赵新颖"," 屈锋"],"authors":"陈尔凝, 赵新颖, 屈锋","authorsCn":"陈尔凝, 赵新颖, 屈锋","authorsEn":"CHEN  Erning, ZHAO  Xinying, QU  Feng","categoryName":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameCn":"特别策划:多尺度靶标的核酸适配体筛选研究进展专栏","categoryNameEn":"","citation":"陈尔凝, 赵新颖, 屈锋. 细菌的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 389-396. doi: 10.3724/SP.J.1123.2015.12021.","citationCn":"陈尔凝, 赵新颖, 屈锋. 细菌的核酸适配体筛选的研究进展. 色谱, 2016, 34(4): 389-396. doi: 10.3724/SP.J.1123.2015.12021.","citationEn":"陈尔凝, 赵新颖, 屈锋. Research advances of aptamers selection for bacterium targets. Chinese Journal of Chromatography, 2016, 34(4): 389-396. doi: 10.3724/SP.J.1123.2015.12021.","doi":"10.3724/SP.J.1123.2015.12021","figContent":"","figList":[],"firstFig":"","fpage":"389","highCitedState":"0","htmlCount":178,"htmlFile":"","id":"343","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"核酸适配体, 细菌, 筛选, 指数富集配体系统进化技术, 综述","keywordCn":"核酸适配体, 细菌, 筛选, 指数富集配体系统进化技术, 综述","keywordCnList":["核酸适配体"," 细菌"," 筛选"," 指数富集配体系统进化技术"," 综述"],"keywordEn":"aptamer, bacteria, selection, systematic evolution of ligands by exponential enrichment (SELEX), review","keywordEnList":["aptamer"," bacteria"," selection"," systematic evolution of ligands by exponential enrichment (SELEX)"," review"],"keywordList":["核酸适配体"," 细菌"," 筛选"," 指数富集配体系统进化技术"," 综述"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"396","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":850,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"细菌的核酸适配体筛选的研究进展","titleCn":"细菌的核酸适配体筛选的研究进展","titleEn":"Research advances of aptamers selection for bacterium targets","viewCount":1513,"volume":"34","year":2016}],"研究论文":[{"abstractInfo":"基于亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱系统,建立了奶粉中氟乙酸钠的快速筛查和定量检测方法。奶粉样品用水溶解后经正己烷脱脂,用6mol/L硫酸调节pH值,经乙腈提取后用亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱进行分析。结果表明,该方法在1~50μg/kg范围内线性关系良好,定量限为1μg/kg,平均回收率为85.5%~106%,相对标准偏差为5.20%~13.6%。该方法可用于配方奶粉、脱脂奶粉和乳清粉等奶粉产品中氟乙酸钠的快速筛查和定量分析。","abstractInfoCn":"基于亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱系统,建立了奶粉中氟乙酸钠的快速筛查和定量检测方法。奶粉样品用水溶解后经正己烷脱脂,用6 mol/L硫酸调节pH值,经乙腈提取后用亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱进行分析。结果表明,该方法在1~50 μg/kg范围内线性关系良好,定量限为1 μg/kg,平均回收率为85.5%~106%,相对标准偏差为5.20%~13.6%。该方法可用于配方奶粉、脱脂奶粉和乳清粉等奶粉产品中氟乙酸钠的快速筛查和定量分析。","abstractInfoEn":"A method for the determination of sodium fluoroacetate in milk powder by hydrophilic interaction chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry was developed. After the sample was dissolved by water, sodium fluoroacetate was degreased by hexane. The pH value of the extractant was adjusted by 6 mol/L H<sub>2<\/sub>SO<sub>4<\/sub>. After extracted by acetonitrile, the extractant was determined by hydrophilic interaction chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry. The linear range of the sodium fluoroacetate was from 1 to 50 μg/kg, with the correlation coefficients greater than 0.99. By detecting the spiked samples, the limits of quantification were 1 μg/kg, while the recoveries were in the range of 85.5%-106% with the relative standard deviations (RSDs) between 5.20% and 13.6%. This method can be used for the quick determination of sodium fluoroacetate in powder formula, skimmed milk powder and whey powder.","articleNo":"sp1511040","authorCnList":["赵善贞"," 伊雄海"," 时逸吟"," 徐牛生"," 张凯元"," 邓晓军"],"authorEnList":["ZHAO  Shanzhen"," YI  Xionghai"," SHI  Yiyin"," XU  Niusheng"," ZHANG  Kaiyuan"," DENG  Xiaojun"],"authorList":["赵善贞"," 伊雄海"," 时逸吟"," 徐牛生"," 张凯元"," 邓晓军"],"authors":"赵善贞, 伊雄海, 时逸吟, 徐牛生, 张凯元, 邓晓军","authorsCn":"赵善贞, 伊雄海, 时逸吟, 徐牛生, 张凯元, 邓晓军","authorsEn":"ZHAO  Shanzhen, YI  Xionghai, SHI  Yiyin, XU  Niusheng, ZHANG  Kaiyuan, DENG  Xiaojun","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"赵善贞, 伊雄海, 时逸吟, 徐牛生, 张凯元, 邓晓军. 亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱检测奶粉中的氟乙酸钠. 色谱, 2016, 34(4): 397-400. doi: 10.3724/SP.J.1123.2015.11040.","citationCn":"赵善贞, 伊雄海, 时逸吟, 徐牛生, 张凯元, 邓晓军. 亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱检测奶粉中的氟乙酸钠. 色谱, 2016, 34(4): 397-400. doi: 10.3724/SP.J.1123.2015.11040.","citationEn":"赵善贞, 伊雄海, 时逸吟, 徐牛生, 张凯元, 邓晓军. Determination of sodium fluoroacetate in milk powder by hydrophilic interaction chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry. Chinese Journal of Chromatography, 2016, 34(4): 397-400. doi: 10.3724/SP.J.1123.2015.11040.","doi":"10.3724/SP.J.1123.2015.11040","figContent":"","figList":[],"firstFig":"","fpage":"397","highCitedState":"0","htmlCount":32,"htmlFile":"","id":"344","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"亲水相互作用色谱, 四极杆/静电场轨道阱高分辨质谱, 氟乙酸钠, 奶粉","keywordCn":"亲水相互作用色谱, 四极杆/静电场轨道阱高分辨质谱, 氟乙酸钠, 奶粉","keywordCnList":["亲水相互作用色谱"," 四极杆/静电场轨道阱高分辨质谱"," 氟乙酸钠"," 奶粉"],"keywordEn":"hydrophilic interaction chromatography, quadrupole/orbitrap high resolution mass spectrometry (HR-MS), sodium fluoroacetate, milk powder","keywordEnList":["hydrophilic interaction chromatography"," quadrupole/orbitrap high resolution mass spectrometry (HR-MS)"," sodium fluoroacetate"," milk powder"],"keywordList":["亲水相互作用色谱"," 四极杆/静电场轨道阱高分辨质谱"," 氟乙酸钠"," 奶粉"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"400","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":798,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱检测奶粉中的氟乙酸钠","titleCn":"亲水相互作用色谱-四极杆/静电场轨道阱高分辨质谱检测奶粉中的氟乙酸钠","titleEn":"Determination of sodium fluoroacetate in milk powder by hydrophilic interaction chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry","viewCount":555,"volume":"34","year":2016},{"abstractInfo":"建立了原多甲藻酸毒素(azaspiracids,AZAs)及其代谢产物的液相色谱-串联质谱检测方法,考察了目标代谢产物检测方法和非目标多离子检测方法,将二级质谱图与标准品谱库进行比对,从而获得高精准定性。通过对产毒藻、蓄积代谢实验样品和实际阳性样品综合分析,共检出11种AZAs,其中包括AZA-2,3,6,11,12,16,17,28和36,以及AZAs的谷胱甘肽结合型代谢产物。结果发现,目标代谢产物检测法可无偏差地筛出常规代谢物,而且对低浓度代谢产物表现出更佳的响应。本方法重现性好,数据分析简单,对操作人员的专业要求不高,更适合AZAs的监控要求。","abstractInfoCn":"建立了原多甲藻酸毒素(azaspiracids,AZAs)及其代谢产物的液相色谱-串联质谱检测方法,考察了目标代谢产物检测方法和非目标多离子检测方法,将二级质谱图与标准品谱库进行比对,从而获得高精准定性。通过对产毒藻、蓄积代谢实验样品和实际阳性样品综合分析,共检出11种AZAs,其中包括AZA-2,3,6,11,12,16,17,28和36,以及AZAs的谷胱甘肽结合型代谢产物。结果发现,目标代谢产物检测法可无偏差地筛出常规代谢物,而且对低浓度代谢产物表现出更佳的响应。本方法重现性好,数据分析简单,对操作人员的专业要求不高,更适合AZAs的监控要求。","abstractInfoEn":"A liquid chromatography-tandem mass spectrometry method was developed for the analysis of azaspiracids and their metabolites. Both target metabolites and non-objective compounds detection methods were investigated, and the secondary mass spectra were compared with the standard spectra libraries to achieve high precision qualitative analysis. As a result, 11 metabolites including AZA-2, 3, 6, 11, 12, 16, 17, 28 and 36, as well as the glutathione conjugated metabolites of AZAs were analyzed in toxin-producing algae, accumulated samples of metabolic experiments and positive samples. Conventional metabolites were unbiased detected by target metabolites detection method, and showed a better response to low concentration metabolites. The method has excellent reproducibility, and is easy for operators. It can be used for the routine monitoring of AZAs.","articleNo":"sp1511043","authorCnList":["吴海燕"," 郭萌萌"," 赵春霞"," 谭志军"," 顾海峰"," 翟毓秀"," 卢立娜"],"authorEnList":["WU  Haiyan"," GUO  Mengmeng"," ZHAO  Chunxia"," TAN  Zhijun"," GU  Haifeng"," ZHAI  Yuxiu"," LU  Lina"],"authorList":["吴海燕"," 郭萌萌"," 赵春霞"," 谭志军"," 顾海峰"," 翟毓秀"," 卢立娜"],"authors":"吴海燕, 郭萌萌, 赵春霞, 谭志军, 顾海峰, 翟毓秀, 卢立娜","authorsCn":"吴海燕, 郭萌萌, 赵春霞, 谭志军, 顾海峰, 翟毓秀, 卢立娜","authorsEn":"WU  Haiyan, GUO  Mengmeng, ZHAO  Chunxia, TAN  Zhijun, GU  Haifeng, ZHAI  Yuxiu, LU  Lina","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"吴海燕, 郭萌萌, 赵春霞, 谭志军, 顾海峰, 翟毓秀, 卢立娜. 液相色谱-串联质谱法筛查原多甲藻酸毒素及其代谢产物. 色谱, 2016, 34(4): 401-406. doi: 10.3724/SP.J.1123.2015.11043.","citationCn":"吴海燕, 郭萌萌, 赵春霞, 谭志军, 顾海峰, 翟毓秀, 卢立娜. 液相色谱-串联质谱法筛查原多甲藻酸毒素及其代谢产物. 色谱, 2016, 34(4): 401-406. doi: 10.3724/SP.J.1123.2015.11043.","citationEn":"吴海燕, 郭萌萌, 赵春霞, 谭志军, 顾海峰, 翟毓秀, 卢立娜. Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites. Chinese Journal of Chromatography, 2016, 34(4): 401-406. doi: 10.3724/SP.J.1123.2015.11043.","doi":"10.3724/SP.J.1123.2015.11043","figContent":"","figList":[],"firstFig":"","fpage":"401","highCitedState":"0","htmlCount":27,"htmlFile":"","id":"345","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"液相色谱-串联质谱法, 原多甲藻酸毒素, 代谢产物, 定性分析","keywordCn":"液相色谱-串联质谱法, 原多甲藻酸毒素, 代谢产物, 定性分析","keywordCnList":["液相色谱-串联质谱法"," 原多甲藻酸毒素"," 代谢产物"," 定性分析"],"keywordEn":"liquid chromatography-tandem mass spectrometry (LC-MS/MS), azaspiracids (AZAs), metabolites, qualitative analysis","keywordEnList":["liquid chromatography-tandem mass spectrometry (LC-MS/MS)"," azaspiracids (AZAs)"," metabolites"," qualitative analysis"],"keywordList":["液相色谱-串联质谱法"," 原多甲藻酸毒素"," 代谢产物"," 定性分析"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"406","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":1171,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"液相色谱-串联质谱法筛查原多甲藻酸毒素及其代谢产物","titleCn":"液相色谱-串联质谱法筛查原多甲藻酸毒素及其代谢产物","titleEn":"Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites","viewCount":514,"volume":"34","year":2016},{"abstractInfo":"建立了磁性固相萃取-高效液相色谱-串联质谱同时测定环境水样中四环素类抗生素的方法。以6种四环素类抗生素(差向四环素、土霉素、四环素、去甲金霉素、金霉素和脱水四环素)为目标化合物,考察并优化了吸附和解吸条件,确定了最佳萃取条件。萃取后的目标化合物经ZORBAXEclipsePlusC<sub>18<\/sub>柱分离,用高效液相色谱-串联质谱在多反应监测(MRM)模式下进行检测。在优化的条件下,6种四环素在1~100μg/L范围内线性关系良好,线性相关系数为0.9967~0.9993,检出限为2.44~25.21ng/L,样品加标回收率为80.6%~90.0%,日内相对标准偏差(RSDs)为0.6%~2.5%,日间RSDs为1.1%~7.1%。该方法灵敏度高、背景干扰低,适用于环境水样中6种痕量四环素类抗生素的同时检测。","abstractInfoCn":"建立了磁性固相萃取-高效液相色谱-串联质谱同时测定环境水样中四环素类抗生素的方法。以6种四环素类抗生素(差向四环素、土霉素、四环素、去甲金霉素、金霉素和脱水四环素)为目标化合物,考察并优化了吸附和解吸条件,确定了最佳萃取条件。萃取后的目标化合物经ZORBAX Eclipse Plus C<sub>18<\/sub>柱分离,用高效液相色谱-串联质谱在多反应监测(MRM)模式下进行检测。在优化的条件下,6种四环素在1~100 μg/L范围内线性关系良好,线性相关系数为0.9967~0.9993,检出限为2.44~25.21 ng/L,样品加标回收率为80.6%~90.0%,日内相对标准偏差(RSDs)为0.6%~2.5%,日间RSDs为1.1%~7.1%。该方法灵敏度高、背景干扰低,适用于环境水样中6种痕量四环素类抗生素的同时检测。","abstractInfoEn":"A method has been developed for the simultaneous determination of tetracycline antibiotics in environmental water by magnetic solid phase extraction and high performance liquid chromatography-tandem mass spectrometry. Several important parameters affecting the magnetic solid phase extraction procedure have been optimized for the best recovery. The eluate was analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) under multiple reaction monitoring (MRM) mode with a ZORBAX Eclipse Plus C<sub>18<\/sub> column. Under the optimized conditions, the six tetracycline antibiotics (4-epitetracycline, oxytetracycline, tetracycline, demclocycline, chlortetracycline, anhydrotetracycline) showed good linearities in the range of 1-100 μg/L with the correlation coefficients of 0.9967-0.9993. The limits of detection (LODs) were in the range of 2.44 ng/L to 25.21 ng/L. The spiked recoveries were 80.6%-90.0%. The intra-day relative standard deviations (RSDs) were 0.6%-2.5%, while the inter-day RSDs were 1.1%-7.1%. The method has low background and high sensitivity. It was successfully applied to the simultaneous determination of the six trace tetracycline antibiotics in environmental water.","articleNo":"sp1511038","authorCnList":["国明"," 于峰"," 贾科玲"," 李姣"," 孙海"],"authorEnList":["GUO  Ming"," YU  Feng"," JIA  Keling"," LI  Jiao"," SUN  Hai"],"authorList":["国明"," 于峰"," 贾科玲"," 李姣"," 孙海"],"authors":"国明, 于峰, 贾科玲, 李姣, 孙海","authorsCn":"国明, 于峰, 贾科玲, 李姣, 孙海","authorsEn":"GUO  Ming, YU  Feng, JIA  Keling, LI  Jiao, SUN  Hai","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"国明, 于峰, 贾科玲, 李姣, 孙海. 磁性固相萃取-高效液相色谱-串联质谱法测定环境水样中的四环素类抗生素. 色谱, 2016, 34(4): 407-413. doi: 10.3724/SP.J.1123.2015.11038.","citationCn":"国明, 于峰, 贾科玲, 李姣, 孙海. 磁性固相萃取-高效液相色谱-串联质谱法测定环境水样中的四环素类抗生素. 色谱, 2016, 34(4): 407-413. doi: 10.3724/SP.J.1123.2015.11038.","citationEn":"国明, 于峰, 贾科玲, 李姣, 孙海. Determination of tetracycline antibiotics in environmental water using magnetic solid phase extraction combined with high performance liquid chromatography- tandem mass spectrometry. Chinese Journal of Chromatography, 2016, 34(4): 407-413. doi: 10.3724/SP.J.1123.2015.11038.","doi":"10.3724/SP.J.1123.2015.11038","figContent":"","figList":[],"firstFig":"","fpage":"407","highCitedState":"0","htmlCount":36,"htmlFile":"","id":"346","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"磁性固相萃取, 高效液相色谱-串联质谱, 四环素类抗生素, 环境水样","keywordCn":"磁性固相萃取, 高效液相色谱-串联质谱, 四环素类抗生素, 环境水样","keywordCnList":["磁性固相萃取"," 高效液相色谱-串联质谱"," 四环素类抗生素"," 环境水样"],"keywordEn":"magnetic solid phase extraction, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), tetracycline antibiotics, environmental water","keywordEnList":["magnetic solid phase extraction"," high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)"," tetracycline antibiotics"," environmental water"],"keywordList":["磁性固相萃取"," 高效液相色谱-串联质谱"," 四环素类抗生素"," 环境水样"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"413","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":1053,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"磁性固相萃取-高效液相色谱-串联质谱法测定环境水样中的四环素类抗生素","titleCn":"磁性固相萃取-高效液相色谱-串联质谱法测定环境水样中的四环素类抗生素","titleEn":"Determination of tetracycline antibiotics in environmental water using magnetic solid phase extraction combined with high performance liquid chromatography- tandem mass spectrometry","viewCount":561,"volume":"34","year":2016},{"abstractInfo":"为了多层面探讨共培养微环境诱导法定向诱导骨髓间充质干细胞(MSCs)心肌样分化的可行性,取第3代MSCs与原代心肌细胞(CMs)进行共培养。在显微镜下观察诱导1周后的MSCs形态学变化,用免疫荧光和实时荧光定量聚合酶链式反应(RT-PCR)分别检测诱导的MSCs中心肌肌钙蛋白I(cTnI)、<i>α<\/i>-肌动蛋白(<i>α<\/i>-actin)、Nkx-2.5和GATA-4的基因表达变化情况。采用超高效液相色谱-串联质谱(UPLC-MS/MS)分别检测诱导组和对照组的代谢产物。诱导1周后的MSCs形态呈心肌样改变,cTnI、<i>α<\/i>-actin、Nkx-2.5和GATA-4的基因表达均明显升高,正交偏最小二乘法判别分析(OPLS-DA)模型显示诱导的MSCs代谢物向CMs转变趋势明显。通过多元和单元统计分析筛选差异变量,根据一级质谱和二级质谱比对结果,最终确定12种差异代谢物。与未经诱导的MSCs相比,经诱导的MSCs与CMs中变化趋势相同的差异代谢物有7种,变化趋势不同的差异代谢物有5种。实验结果表明,无论从形态、基因、蛋白质还是代谢层面看,MSCs通过与CMs间接接触共培养后均发生了心肌样改变,但是与CMs仍存在差异。","abstractInfoCn":"为了多层面探讨共培养微环境诱导法定向诱导骨髓间充质干细胞(MSCs)心肌样分化的可行性,取第3代MSCs与原代心肌细胞(CMs)进行共培养。在显微镜下观察诱导1周后的MSCs形态学变化,用免疫荧光和实时荧光定量聚合酶链式反应(RT-PCR)分别检测诱导的MSCs中心肌肌钙蛋白I(cTnI)、<i>α<\/i>-肌动蛋白(<i>α<\/i>-actin)、Nkx-2.5和GATA-4的基因表达变化情况。采用超高效液相色谱-串联质谱(UPLC-MS/MS)分别检测诱导组和对照组的代谢产物。诱导1周后的MSCs形态呈心肌样改变,cTnI、<i>α<\/i>-actin、Nkx-2.5和GATA-4的基因表达均明显升高,正交偏最小二乘法判别分析(OPLS-DA)模型显示诱导的MSCs代谢物向CMs转变趋势明显。通过多元和单元统计分析筛选差异变量,根据一级质谱和二级质谱比对结果,最终确定12种差异代谢物。与未经诱导的MSCs相比,经诱导的MSCs与CMs中变化趋势相同的差异代谢物有7种,变化趋势不同的差异代谢物有5种。实验结果表明,无论从形态、基因、蛋白质还是代谢层面看,MSCs通过与CMs间接接触共培养后均发生了心肌样改变,但是与CMs仍存在差异。","abstractInfoEn":"The objective of this research is to investigate the feasibility of cardiac differentiation of bone marrow mesenchymal stem cells (MSCs) by co-culture with cardiomyocytes (CMs) in vitro. The third generation of MSCs from bone marrow and CMs were co-cultured indirectly in a transwell. One week later, the expressions of muscle-specific markers (cardiac troponin I and <i>α<\/i>-actin) by immunofluorescence staining and the gene expressions of transcription factors (Nkx-2.5 and GATA-4) were measured by real-time polymerase chain reaction (RT-PCR). Then, orthogonal partial least squares discriminant analysis (OPLS-DA) models were employed to confirm the difference among MSCs, induced MSCs and CMs by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. The distinctive changes were identified by multivariate analysis and variate analysis, and the changed metabolites were identified by MS and MS/MS. One week after co-cultured with CMs indirectly, the specific myocardium morphology of MSCs was observed under microscope. The positive immunofluorescence stainings against cTnI and <i>α<\/i>-actin were detected, and the positive expressions of the transcription factors Nkx-2.5 and GATA-4 were measured by RT-PCR. In OPLS-DA mode, obvious trend of cardiac differentiation of MSCs can be seen, and seven metabolites were tested both in induced MSCs and CMs, but five metabolites were tested in induced MSCs or CMs. Cardiac differentiation of MSCs can be induced by co-cultured with CMs indirectly in vitro. However, metabolism difference still existed between induced MSCs and CMs.","articleNo":"sp1601006","authorCnList":["何林静"," 邓伏雪"," 胡云凤"," 朱卫文"," 伍家燕"," 常静"],"authorEnList":["HE  Linjing"," DENG  Fuxue"," HU  Yunfeng"," ZHU  Weiwen"," WU  Jiayan"," CHANG  Jing"],"authorList":["何林静"," 邓伏雪"," 胡云凤"," 朱卫文"," 伍家燕"," 常静"],"authors":"何林静, 邓伏雪, 胡云凤, 朱卫文, 伍家燕, 常静","authorsCn":"何林静, 邓伏雪, 胡云凤, 朱卫文, 伍家燕, 常静","authorsEn":"HE  Linjing, DENG  Fuxue, HU  Yunfeng, ZHU  Weiwen, WU  Jiayan, CHANG  Jing","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"何林静, 邓伏雪, 胡云凤, 朱卫文, 伍家燕, 常静. 多层面验证共培养微环境诱导法诱导骨髓间充质干细胞心肌样分化. 色谱, 2016, 34(4): 414-421. doi: 10.3724/SP.J.1123.2016.01006.","citationCn":"何林静, 邓伏雪, 胡云凤, 朱卫文, 伍家燕, 常静. 多层面验证共培养微环境诱导法诱导骨髓间充质干细胞心肌样分化. 色谱, 2016, 34(4): 414-421. doi: 10.3724/SP.J.1123.2016.01006.","citationEn":"何林静, 邓伏雪, 胡云凤, 朱卫文, 伍家燕, 常静. Feasibility of cardiac differentiation of bone marrow mesenchymal stem cells induced by co-culture indirectly with cardiomyocytes in several dimensions. Chinese Journal of Chromatography, 2016, 34(4): 414-421. doi: 10.3724/SP.J.1123.2016.01006.","doi":"10.3724/SP.J.1123.2016.01006","figContent":"","figList":[],"firstFig":"","fpage":"414","highCitedState":"0","htmlCount":44,"htmlFile":"","id":"347","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"共培养微环境, 代谢组学, 心肌样分化, 骨髓间充质干细胞, 心肌细胞","keywordCn":"共培养微环境, 代谢组学, 心肌样分化, 骨髓间充质干细胞, 心肌细胞","keywordCnList":["共培养微环境"," 代谢组学"," 心肌样分化"," 骨髓间充质干细胞"," 心肌细胞"],"keywordEn":"co-culture microenvironment, metabonomics, myocardium-like differentiation, bone marrow mesenchymal stem cells (MSCs), cardiomyocytes (CMs)","keywordEnList":["co-culture microenvironment"," metabonomics"," myocardium-like differentiation"," bone marrow mesenchymal stem cells (MSCs)"," cardiomyocytes (CMs)"],"keywordList":["共培养微环境"," 代谢组学"," 心肌样分化"," 骨髓间充质干细胞"," 心肌细胞"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"421","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":2835,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"多层面验证共培养微环境诱导法诱导骨髓间充质干细胞心肌样分化","titleCn":"多层面验证共培养微环境诱导法诱导骨髓间充质干细胞心肌样分化","titleEn":"Feasibility of cardiac differentiation of bone marrow mesenchymal stem cells induced by co-culture indirectly with cardiomyocytes in several dimensions","viewCount":552,"volume":"34","year":2016},{"abstractInfo":"在考察了荧光胺、邻苯二甲醛、异硫氰酸荧光素和2,3-萘二醛等对磺胺类药物衍生效果的基础上,建立了采用改良QuEChERS方法进行样品前处理,荧光胺在线柱后衍生,高效液相色谱-荧光检测法测定牛肉中16种磺胺残留量的方法。牛肉样品经1%(v/v)乙酸乙腈溶液提取,改良QuEChERS方法净化后取上清液进样,与荧光胺柱后在线衍生,荧光检测器检测。实验结果表明,16种磺胺类药物在0.024~2.533mg/L范围内线性关系良好,相关系数(<i>r<\/i>)大于0.992,检出限为1.6~8.2μg/kg,平均加标回收率范围为66.6%~109.5%,相对标准偏差为0.9%~9.9%。该方法快速简便、灵敏度高、净化效果好,可用于牛肉中16种磺胺类药物的快速测定。","abstractInfoCn":"在考察了荧光胺、邻苯二甲醛、异硫氰酸荧光素和2,3-萘二醛等对磺胺类药物衍生效果的基础上,建立了采用改良QuEChERS方法进行样品前处理,荧光胺在线柱后衍生,高效液相色谱-荧光检测法测定牛肉中16种磺胺残留量的方法。牛肉样品经1%(v/v)乙酸乙腈溶液提取,改良QuEChERS方法净化后取上清液进样,与荧光胺柱后在线衍生,荧光检测器检测。实验结果表明,16种磺胺类药物在0.024~2.533 mg/L范围内线性关系良好,相关系数(<i>r<\/i>)大于0.992,检出限为1.6~8.2 μg/kg,平均加标回收率范围为66.6%~109.5%,相对标准偏差为0.9%~9.9%。该方法快速简便、灵敏度高、净化效果好,可用于牛肉中16种磺胺类药物的快速测定。","abstractInfoEn":"In this study, we investigated the effects of the four kinds of derivatization reagents, including fluorescamine, <i>o<\/i>-phthaladehyde, fluorescein isothiocyanate isomer and 2, 3-naphthalenedicarboxaldehyde. A high performance liquid chromatography with fluorescence detection method for the rapid determination of 16 sulfonamide residues in beef was developed by using improved QuEChERS method for sample pretreatment and fluorescamine for online post-column derivatization. The beef samples were extracted with acetonitrile containing 1%(v/v) acetic acid, cleaned-up by QuEChERS method, injected for online post-column derivatization with fluorescamine, and then analysed with fluorescence detection. Under the optimized conditions of QuEChERS and chromatography, the 16 sulfonamide residues showed good linearities in the range of 0.024-2.533 mg/L with the correlation coefficients (<i>r<\/i>) higher than 0.992. The limits of detection of the method were 1.6 to 8.2 μg/kg. The average recoveries of the 16 sulfonamide residues were in the range of 66.6%-109.5% with the relative standard deviations from 0.9% to 9.9%. The method has the advantages of rapidity, simplicity, high sensitivity and better purification effect. It is suitable for the rapid determination of the sulfonamide residues in beef.","articleNo":"sp1511020","authorCnList":["许旭"," 肖远灿"," 耿丹丹"," 皮立"," 董琦"," 胡风祖"],"authorEnList":["XU  Xu"," XIAO  Yuancan"," GENG  Dandan"," PI  Li"," DONG  Qi"," HU  Fengzu"],"authorList":["许旭"," 肖远灿"," 耿丹丹"," 皮立"," 董琦"," 胡风祖"],"authors":"许旭, 肖远灿, 耿丹丹, 皮立, 董琦, 胡风祖","authorsCn":"许旭, 肖远灿, 耿丹丹, 皮立, 董琦, 胡风祖","authorsEn":"XU  Xu, XIAO  Yuancan, GENG  Dandan, PI  Li, DONG  Qi, HU  Fengzu","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"许旭, 肖远灿, 耿丹丹, 皮立, 董琦, 胡风祖. 在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留. 色谱, 2016, 34(4): 422-428. doi: 10.3724/SP.J.1123.2015.11020.","citationCn":"许旭, 肖远灿, 耿丹丹, 皮立, 董琦, 胡风祖. 在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留. 色谱, 2016, 34(4): 422-428. doi: 10.3724/SP.J.1123.2015.11020.","citationEn":"许旭, 肖远灿, 耿丹丹, 皮立, 董琦, 胡风祖. Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization. Chinese Journal of Chromatography, 2016, 34(4): 422-428. doi: 10.3724/SP.J.1123.2015.11020.","doi":"10.3724/SP.J.1123.2015.11020","figContent":"","figList":[],"firstFig":"","fpage":"422","highCitedState":"0","htmlCount":82,"htmlFile":"","id":"348","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"QuEChERS, 荧光胺, 在线柱后衍生, 高效液相色谱-荧光检测法, 磺胺类药物, 牛肉","keywordCn":"QuEChERS, 荧光胺, 在线柱后衍生, 高效液相色谱-荧光检测法, 磺胺类药物, 牛肉","keywordCnList":["QuEChERS"," 荧光胺"," 在线柱后衍生"," 高效液相色谱-荧光检测法"," 磺胺类药物"," 牛肉"],"keywordEn":"QuEChERS, fluorescamine, online post-column derivatization, high performance liquid chromatography-fluorescence detection (HPLC-FLR), sulfonamides, beef","keywordEnList":["QuEChERS"," fluorescamine"," online post-column derivatization"," high performance liquid chromatography-fluorescence detection (HPLC-FLR)"," sulfonamides"," beef"],"keywordList":["QuEChERS"," 荧光胺"," 在线柱后衍生"," 高效液相色谱-荧光检测法"," 磺胺类药物"," 牛肉"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"428","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":3,"pdfSize":846,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留","titleCn":"在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留","titleEn":"Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization","viewCount":728,"volume":"34","year":2016},{"abstractInfo":"分别采用电子轰击(EI)和正化学电离(PCI)两种离子源技术建立了气相色谱-质谱联用法(GC-MS)同时测定橄榄油中3,5-菜甾二烯、3,5,22-豆甾三烯和3,5-豆甾二烯3种甾醇烯含量的方法,并对这两种方法进行了比较。样品经石油醚溶解,硅胶柱净化后,分别采用GC-EI/MS和GC-PCI/MS分时段选择离子监测模式进行测定,以3,5-胆甾二烯为内标进行定量。结果表明,两种方法的线性、准确度、精密度、灵敏度均较好。3,5-菜甾二烯、3,5,22-豆甾三烯和3,5-豆甾二烯分别在0.024~0.48、0.02~0.50和0.03~0.75mg/L浓度范围内线性关系良好(<i>r<\/i>>0.999)。在3个加标水平下,GC-EI/MS和GC-PCI/MS的平均回收率分别为88.7%~99.5%、87.1%~109.2%,两种检测方法的相对标准偏差(RSD,<i>n<\/i>=6)均不超过8.3%。定量限(<i>S<\/i>/<i>N<\/i>=10)分别为0.03mg/kg(EI),0.03~0.10mg/kg(PCI)。通过对两种方法的比较研究发现,EI能提供更多碎片离子和结构信息,而PCI中则主要为准分子离子及反应气加合离子。应用于样品中甾醇烯的测定时,PCI的选择性和抗干扰能力明显优于EI。两种方法可相互补充和替代应用于日常检测中。","abstractInfoCn":"分别采用电子轰击(EI)和正化学电离(PCI)两种离子源技术建立了气相色谱-质谱联用法(GC-MS)同时测定橄榄油中3,5-菜甾二烯、3,5,22-豆甾三烯和3,5-豆甾二烯3种甾醇烯含量的方法,并对这两种方法进行了比较。样品经石油醚溶解,硅胶柱净化后,分别采用GC-EI/MS和GC-PCI/MS分时段选择离子监测模式进行测定,以3,5-胆甾二烯为内标进行定量。结果表明,两种方法的线性、准确度、精密度、灵敏度均较好。3,5-菜甾二烯、3,5,22-豆甾三烯和3,5-豆甾二烯分别在0.024~0.48、0.02~0.50和0.03~0.75 mg/L浓度范围内线性关系良好(<i>r<\/i>>0.999)。在3个加标水平下,GC-EI/MS和GC-PCI/MS的平均回收率分别为88.7%~99.5%、87.1%~109.2%,两种检测方法的相对标准偏差(RSD,<i>n<\/i>=6)均不超过8.3%。定量限(<i>S<\/i>/<i>N<\/i>=10)分别为0.03 mg/kg(EI),0.03~0.10 mg/kg(PCI)。通过对两种方法的比较研究发现,EI能提供更多碎片离子和结构信息,而PCI中则主要为准分子离子及反应气加合离子。应用于样品中甾醇烯的测定时,PCI的选择性和抗干扰能力明显优于EI。两种方法可相互补充和替代应用于日常检测中。","abstractInfoEn":"The gas chromatography-mass spectrometric (GC-MS) method with two different ionization techniques, electron impact (EI) and positive chemical ionization (PCI), have been developed for the simultaneous determination of three sterenes in olive oil. The olive oil sample was dissolved in petroleum ether, followed by cleanup on a silica gel column and finally analyzed by GC-EI/MS and GC-PCI/MS in selected ion monitoring mode. The quantitation was performed by internal standard calibration with cholesta-3, 5-diene as internal standard. These two methods were compared in terms of linearity, accuracy, precision and sensitivity. Good linearities were obtained with correlation coefficients (<i>r<\/i>) higher than 0.999 for campesta-3, 5-diene in the range of 0.024 to 0.48 mg/L, stigmasta-3, 5, 22-triene in the range of 0.02 to 0.50 mg/L and stigmasta-3, 5-diene in the range of 0.03 to 0.75 mg/L. The accuracy test was carried out at three spiked levels in extra virgin olive oil. The average recoveries ranged from 88.7% to 99.5% (EI) and 87.1% to 109.2% (PCI), with relative standard deviations (RSDs, <i>n<\/i>=6) not higher than 8.3%. Limits of quantification (LOQs, <i>S<\/i>/<i>N<\/i>=10) were 0.03 mg/kg (EI) and 0.03-0.10 mg/kg (PCI). Comparative analysis showed a large number of fragment ions could be obtained in EI mode while quasi-molecular ions or other adduct molecular ions were the most abundant ion formed in the PCI mode. Besides, PCI has a better selectivity than EI when applied to real samples. In conclusion, these two ionization techniques are complementary and alternative in the routine analysis of sterenes in olive oil.","articleNo":"sp1512016","authorCnList":["魏雪缘"," 沈伟健"," 余可垚"," 张建"," 陆慧媛"," 吴斌"," 沈崇钰"," 赵增运"," 柳菡"," 邓晓军"," 王岁楼"],"authorEnList":["WEI  Xueyuan"," SHEN  Weijian"," YU  Keyao"," ZHANG  Jian"," LU  Huiyuan"," WU  Bin"," SHEN  Chongyu"," ZHAO  Zengyun"," LIU  Han"," DENG  Xiaojun"," WANG  Suilou"],"authorList":["魏雪缘"," 沈伟健"," 余可垚"," 张建"," 陆慧媛"," 吴斌"," 沈崇钰"," 赵增运"," 柳菡"," 邓晓军"," 王岁楼"],"authors":"魏雪缘, 沈伟健, 余可垚, 张建, 陆慧媛, 吴斌, 沈崇钰, 赵增运, 柳菡, 邓晓军, 王岁楼","authorsCn":"魏雪缘, 沈伟健, 余可垚, 张建, 陆慧媛, 吴斌, 沈崇钰, 赵增运, 柳菡, 邓晓军, 王岁楼","authorsEn":"WEI  Xueyuan, SHEN  Weijian, YU  Keyao, ZHANG  Jian, LU  Huiyuan, WU  Bin, SHEN  Chongyu, ZHAO  Zengyun, LIU  Han, DENG  Xiaojun, WANG  Suilou","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"魏雪缘, 沈伟健, 余可垚, 张建, 陆慧媛, 吴斌, 沈崇钰, 赵增运, 柳菡, 邓晓军, 王岁楼. 两种离子化技术气相色谱-质谱法测定橄榄油中甾醇烯类物质的比较. 色谱, 2016, 34(4): 429-435. doi: 10.3724/SP.J.1123.2015.12016.","citationCn":"魏雪缘, 沈伟健, 余可垚, 张建, 陆慧媛, 吴斌, 沈崇钰, 赵增运, 柳菡, 邓晓军, 王岁楼. 两种离子化技术气相色谱-质谱法测定橄榄油中甾醇烯类物质的比较. 色谱, 2016, 34(4): 429-435. doi: 10.3724/SP.J.1123.2015.12016.","citationEn":"魏雪缘, 沈伟健, 余可垚, 张建, 陆慧媛, 吴斌, 沈崇钰, 赵增运, 柳菡, 邓晓军, 王岁楼. A comparative study of two ionization techniques in gas chromatography-mass spectrometric determination of sterenes in olive oil. Chinese Journal of Chromatography, 2016, 34(4): 429-435. doi: 10.3724/SP.J.1123.2015.12016.","doi":"10.3724/SP.J.1123.2015.12016","figContent":"","figList":[],"firstFig":"","fpage":"429","highCitedState":"0","htmlCount":45,"htmlFile":"","id":"349","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"气相色谱-质谱, 甾醇烯, 电子轰击电离, 正化学电离","keywordCn":"气相色谱-质谱, 甾醇烯, 电子轰击电离, 正化学电离","keywordCnList":["气相色谱-质谱"," 甾醇烯"," 电子轰击电离"," 正化学电离"],"keywordEn":"gas chromatography-mass spectrometry (GC-MS), sterenes, electron impact ionization (EI), positive chemical ionization (PCI)","keywordEnList":["gas chromatography-mass spectrometry (GC-MS)"," sterenes"," electron impact ionization (EI)"," positive chemical ionization (PCI)"],"keywordList":["气相色谱-质谱"," 甾醇烯"," 电子轰击电离"," 正化学电离"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"435","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":856,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"两种离子化技术气相色谱-质谱法测定橄榄油中甾醇烯类物质的比较","titleCn":"两种离子化技术气相色谱-质谱法测定橄榄油中甾醇烯类物质的比较","titleEn":"A comparative study of two ionization techniques in gas chromatography-mass spectrometric determination of sterenes in olive oil","viewCount":616,"volume":"34","year":2016},{"abstractInfo":"建立了一种水稻植株、糙米、稻壳、土壤和田水中的杀螟丹气相色谱-火焰光度检测(GC-FPD)方法。样品中的杀螟丹使用稀盐酸提取,然后在碱性条件下使用氯化镍(NiCl<sub>2<\/sub>)将其衍生为沙蚕毒素,最后采用在线连接的支撑液液萃取(SLE)和固相萃取(SPE)进行萃取和富集。在优化好的条件下,杀螟丹在5种空白样品中低、中、高3种添加浓度的回收率为80.0%~114.4%,相对标准偏差(RSD)小于13.7%,表明所建立的方法具有良好的准确度和精密度。将所建立的方法用于大田条件下杀螟丹的残留动态分析,为建立杀螟丹的最大残留限量(MRL)提供参考,同时也可对农药施用技术的安全性进行评价。","abstractInfoCn":"建立了一种水稻植株、糙米、稻壳、土壤和田水中的杀螟丹气相色谱-火焰光度检测(GC-FPD)方法。样品中的杀螟丹使用稀盐酸提取,然后在碱性条件下使用氯化镍(NiCl<sub>2<\/sub>)将其衍生为沙蚕毒素,最后采用在线连接的支撑液液萃取(SLE)和固相萃取(SPE)进行萃取和富集。在优化好的条件下,杀螟丹在5种空白样品中低、中、高3种添加浓度的回收率为80.0%~114.4%,相对标准偏差(RSD)小于13.7%,表明所建立的方法具有良好的准确度和精密度。将所建立的方法用于大田条件下杀螟丹的残留动态分析,为建立杀螟丹的最大残留限量(MRL)提供参考,同时也可对农药施用技术的安全性进行评价。","abstractInfoEn":"Cartap has been widely used as a highly effective pesticide in rice fields for the control of rice stem borer and leaf folde. In this paper, a novel analytical method of cartap in rice plants, husked rice, rice hull, soil and water was developed by gas chromatography-flame photometric detector (GC-FPD). Cartap in these sample matrices was first extracted with dilute hydrochloric acid solution, and then derivatized to nereistoxin with nickel chloride (NiCl<sub>2<\/sub>) under basic condition. At last, nereistoxin was extracted and concentrated by on-line combination of supported liquid-liquid extraction (SLE) and solid phase extraction (SPE). Under the optimal conditions, the recoveries of cartap in these sample matrices spiked at three levels ranged from 80.0% to 114.4% with relative standard deviations (RSDs) less than 13.7%, indicating good accuracy and precision of our proposed method. The proposed method was then applied to the analysis of residue and dissipation of cartap in rice plants, husked rice, rice hull, soil and water under field conditions. The results can provide reference for the establishment of the maximum residue level (MRL) of cartap and estimation of safety of pesticide application technology.","articleNo":"sp1512034","authorCnList":["彭西甜"," 夏虹"," 张仙"," 胡西州"," 彭立军"," 沈菁"],"authorEnList":["PENG  Xitian"," XIA  Hong"," ZHANG  Xian"," HU  Xizhou"," PENG  Lijun"," SHEN  Jing"],"authorList":["彭西甜"," 夏虹"," 张仙"," 胡西州"," 彭立军"," 沈菁"],"authors":"彭西甜, 夏虹, 张仙, 胡西州, 彭立军, 沈菁","authorsCn":"彭西甜, 夏虹, 张仙, 胡西州, 彭立军, 沈菁","authorsEn":"PENG  Xitian, XIA  Hong, ZHANG  Xian, HU  Xizhou, PENG  Lijun, SHEN  Jing","categoryName":"研究论文","categoryNameCn":"研究论文","categoryNameEn":"","citation":"彭西甜, 夏虹, 张仙, 胡西州, 彭立军, 沈菁. 一种新型的杀螟丹分析方法及其在稻田杀螟丹残留动态分析中的应用. 色谱, 2016, 34(4): 436-441. doi: 10.3724/SP.J.1123.2015.12034.","citationCn":"彭西甜, 夏虹, 张仙, 胡西州, 彭立军, 沈菁. 一种新型的杀螟丹分析方法及其在稻田杀螟丹残留动态分析中的应用. 色谱, 2016, 34(4): 436-441. doi: 10.3724/SP.J.1123.2015.12034.","citationEn":"彭西甜, 夏虹, 张仙, 胡西州, 彭立军, 沈菁. Development of a novel analytical method of cartap and its application to the residue and dissipation analysis of cartap in paddy field. Chinese Journal of Chromatography, 2016, 34(4): 436-441. doi: 10.3724/SP.J.1123.2015.12034.","doi":"10.3724/SP.J.1123.2015.12034","figContent":"","figList":[],"firstFig":"","fpage":"436","highCitedState":"0","htmlCount":91,"htmlFile":"","id":"350","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"支撑液液萃取, 气相色谱-火焰光度检测器, 杀螟丹, 水稻, 残留动态","keywordCn":"支撑液液萃取, 气相色谱-火焰光度检测器, 杀螟丹, 水稻, 残留动态","keywordCnList":["支撑液液萃取"," 气相色谱-火焰光度检测器"," 杀螟丹"," 水稻"," 残留动态"],"keywordEn":"supported liquid-liquid extraction (SLE), gas chromatography-flame photometric detector (GC-FPD), cartap, rice, residue and dissipation","keywordEnList":["supported liquid-liquid extraction (SLE)"," gas chromatography-flame photometric detector (GC-FPD)"," cartap"," rice"," residue and dissipation"],"keywordList":["支撑液液萃取"," 气相色谱-火焰光度检测器"," 杀螟丹"," 水稻"," 残留动态"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"441","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":0,"pdfSize":1168,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"一种新型的杀螟丹分析方法及其在稻田杀螟丹残留动态分析中的应用","titleCn":"一种新型的杀螟丹分析方法及其在稻田杀螟丹残留动态分析中的应用","titleEn":"Development of a novel analytical method of cartap and its application to the residue and dissipation analysis of cartap in paddy field","viewCount":779,"volume":"34","year":2016}],"技术与应用":[{"abstractInfo":"建立了离子色谱-柱切换测定土壤浸出液中氟离子的方法。使用柱切换技术,将样品通过高聚物反相色谱柱进行在线预处理,分离氟离子和小分子有机酸,同时除去水溶性有机杂质,采用收集环收集氟离子并进入分析系统分析,消除了测定氟离子时普遍存在的干扰问题。分析系统的淋洗液为KOH溶液,流速为1.0mL/min,采用抑制性电导检测,外标法定量。氟离子的线性范围为0.05~10.0mg/L,相关系数为0.9999,加标回收率为103.4%~105.3%,相对标准偏差为2.0%~2.1%,检出限(<i>S<\/i>/<i>N<\/i>=3)为5.50μg/L。该方法适用于测定复杂基体样品中的氟离子。","abstractInfoCn":"建立了离子色谱-柱切换测定土壤浸出液中氟离子的方法。使用柱切换技术,将样品通过高聚物反相色谱柱进行在线预处理,分离氟离子和小分子有机酸,同时除去水溶性有机杂质,采用收集环收集氟离子并进入分析系统分析,消除了测定氟离子时普遍存在的干扰问题。分析系统的淋洗液为KOH溶液,流速为1.0 mL/min,采用抑制性电导检测,外标法定量。氟离子的线性范围为0.05~10.0 mg/L,相关系数为0.9999,加标回收率为103.4%~105.3%,相对标准偏差为2.0%~2.1%,检出限 (<i>S<\/i>/<i>N<\/i>=3)为5.50 μg/L。该方法适用于测定复杂基体样品中的氟离子。","abstractInfoEn":"A new method was proposed for the determination of fluoride in soil water extract by ion chromatography with column-switching technique. A reversed-phase column was used as a pretreatment column on-line to separate fluoride and organic acids as well as to eliminate organics. Fluoride was collected by a collection loop and delivered to the analytical system. Under the optimum conditions, the linear range of the method for fluoride was 0.05-10.0 mg/L (<i>r<\/i>=0.9999), and the limit of detection (<i>S<\/i>/<i>N<\/i>=3) was 5.50 μg/L. The recovery was in the range of 103.4%-105.3% with the relative standard deviation of 2.0%-2.1%. The method could be applied to determine fluoride in complicated samples.","articleNo":"sp1512025","authorCnList":["姜新华"," 倪承珠"," 朱彬和"," 赵训燕"," 陈素清"," 吕伟德"," 张嘉捷"," 张培敏"," 李刚"," 朱岩"],"authorEnList":["JIANG  Xinhua"," NI  Chengzhu"," ZHU  Binhe"," ZHAO  Xunyan"," CHEN  Suqing"," LV  Weide"," ZHANG  Jiajie"," ZHANG  Peimin"," LI  Gang"," ZHU  Yan"],"authorList":["姜新华"," 倪承珠"," 朱彬和"," 赵训燕"," 陈素清"," 吕伟德"," 张嘉捷"," 张培敏"," 李刚"," 朱岩"],"authors":"姜新华, 倪承珠, 朱彬和, 赵训燕, 陈素清, 吕伟德, 张嘉捷, 张培敏, 李刚, 朱岩","authorsCn":"姜新华, 倪承珠, 朱彬和, 赵训燕, 陈素清, 吕伟德, 张嘉捷, 张培敏, 李刚, 朱岩","authorsEn":"JIANG  Xinhua, NI  Chengzhu, ZHU  Binhe, ZHAO  Xunyan, CHEN  Suqing, LV  Weide, ZHANG  Jiajie, ZHANG  Peimin, LI  Gang, ZHU  Yan","categoryName":"技术与应用","categoryNameCn":"技术与应用","categoryNameEn":"","citation":"姜新华, 倪承珠, 朱彬和, 赵训燕, 陈素清, 吕伟德, 张嘉捷, 张培敏, 李刚, 朱岩. 离子色谱-柱切换法测定土壤浸出液中的氟离子. 色谱, 2016, 34(4): 442-446. doi: 10.3724/SP.J.1123.2015.12025.","citationCn":"姜新华, 倪承珠, 朱彬和, 赵训燕, 陈素清, 吕伟德, 张嘉捷, 张培敏, 李刚, 朱岩. 离子色谱-柱切换法测定土壤浸出液中的氟离子. 色谱, 2016, 34(4): 442-446. doi: 10.3724/SP.J.1123.2015.12025.","citationEn":"姜新华, 倪承珠, 朱彬和, 赵训燕, 陈素清, 吕伟德, 张嘉捷, 张培敏, 李刚, 朱岩. Determination of fluoride in soil water extract by ion chromatography with column-switching technique. Chinese Journal of Chromatography, 2016, 34(4): 442-446. doi: 10.3724/SP.J.1123.2015.12025.","doi":"10.3724/SP.J.1123.2015.12025","figContent":"","figList":[],"firstFig":"","fpage":"442","highCitedState":"0","htmlCount":34,"htmlFile":"","id":"351","issnPpub":"1000-8713","issue":"4","journalPublishId":"sp","journalReferenceCodeType":0,"journalTitleCn":"色谱","journalTitleEn":"Chinese Journal of Chromatography","keyword":"离子色谱, 柱切换, 氟离子, 浸出液, 土壤","keywordCn":"离子色谱, 柱切换, 氟离子, 浸出液, 土壤","keywordCnList":["离子色谱"," 柱切换"," 氟离子"," 浸出液"," 土壤"],"keywordEn":"ion chromatography (IC), column-switching, fluoride, water extract, soil","keywordEnList":["ion chromatography (IC)"," column-switching"," fluoride"," water extract"," soil"],"keywordList":["离子色谱"," 柱切换"," 氟离子"," 浸出液"," 土壤"],"language":"zh","latestInfo":"","latestState":"","latestStateEn":"","lpage":"446","oldUrl":"http://www.chrom-china.com/fileup/HTML","pdfDownCount":1,"pdfSize":1088,"pubDate":"","recommendState":"","releaseState":"1","secCount":0,"title":"离子色谱-柱切换法测定土壤浸出液中的氟离子","titleCn":"离子色谱-柱切换法测定土壤浸出液中的氟离子","titleEn":"Determination of fluoride in soil water extract by ion chromatography with column-switching technique","viewCount":651,"volume":"34","year":2016}]}