Citation: Shen Jiejie, Mao Xuming, Chen Xin'ai, Li Yongquan. Recent Advances in Acyltransferase Domain of Type Ⅰ Polyktide Synthases[J]. Chinese Journal of Organic Chemistry, ;2018, 38(9): 2377-2385. doi: 10.6023/cjoc201806016 shu

Recent Advances in Acyltransferase Domain of Type Ⅰ Polyktide Synthases

Shen Jiejie ^a,b ,
Mao Xuming ^a,b ,
Chen Xin'ai ^a,b ,
Li Yongquan ^a,b,,

a.
Institute of Pharmaceutical Biotechnology, Zhejiang University, Hangzhou 310058
b.
Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou 310058

Corresponding author: Li Yongquan, lyq@zju.edu.cn
Received Date: 12 June 2018
Revised Date: 29 June 2018
Available Online: 16 September 2018
Fund Project: the National Natural Science Foundation of China 31520103901Project supported by the National Natural Science Foundation of China (Nos. 3173002, 31520103901, 31470212, 31571284)the National Natural Science Foundation of China 31470212the National Natural Science Foundation of China 31571284the National Natural Science Foundation of China 3173002

Figures(6)

Most polyketide natural compounds, such as antibiotics, antineoplastics and immunosuppressants, are produced by type Ⅰ polyketide synthases (PKSs). Type Ⅰ PKSs are composed of several catalytic modules, each of which contains iterative domains, such as acyltransferase (AT) domain, for one round of polyketide chain elongation. The recent advances on AT domains of type Ⅰ PKS modules and analyzes their categories, the diverse acyl subunits they transfer, their catalytic mechanisms and their protein structures are summarized. Moreover, the recent progress in AT engineering (AT domains swaps, AT site-directed mutagenesis and trans-AT complementation) for new polyketide derivatives is summarized, to show that the substrate specificity of AT domains is one of the key factors on determining the diversity of polyketide backbones. These works have laid a theoretical foundation for the further development of novel polyketides with multi-functions and in high-yields by AT domain engineering.
- AT domain,
- substrate specificity,
- catalytic mechanism,
- type Ⅰ PKS,
- polyketide
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