Citation: YANG Yang, GUO Xia. Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid[J]. Acta Physico-Chimica Sinica, ;2014, 30(2): 345-350. doi: 10.3866/PKU.WHXB201311282 shu

Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid

YANG Yang ¹ ,
GUO Xia ^2,

1.
1 State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, P. R. China;
2 School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu Province, P. R. China

Received Date: 16 September 2013
Available Online: 28 November 2013
Fund Project: 国家自然科学基金(21073155，21373179) (21073155，21373179)美国NIH(CA129835，CA149363，CA151652，CA129421)资助项目 (CA129835，CA149363，CA151652，CA129421)

The development of synthetic vectors based on low-molecular-weight polyethylenimine (PEI) and the construction of negatively charged vectors with high nucleic acid encapsulation are two of the current research trends in gene delivery. In the present study, we developed a stable and negatively charged lipopolyplex based on oleic acid and a low-molecular-weight PEI (2 kDa); it was formed by simultaneously mixing oleic acid micelles and a polyplex produced from PEI (2 kDa) and oli dsDNA. This lipopolyplex is stable in serum, and because of the low molecular weight of the PEI, the lipopolyplex exhibits very low toxicity. The encapsulation efficiencies of nucleic acids by traditional anionic vectors are low, but very high (more than 80%) by this lipopolyplex. Suitable surface modification with 1,2-distearoryl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethyleneglycol-2000) ammonium salt enabled the lipopolyplex to bind to cells, and a high cellular uptake efficiency (ca 90%) was observed in vitro.
- Polyethylenimine,
- Oleic acid,
- Gene delivery
1. [1]
  
  (1) Xu, L.; Anchordoquy, T. J. Pharmaceutical Sci. 2011, 100,38. doi: 10.1002/jps.22243
2. [2]
  (2) Malam, Y.; Lim, E. J.; Seifalian, A. M. Current Medicinal Chemistry 2011, 18, 1067. doi: 10.2174/092986711794940860
3. [3]
  (3) Guo, X.; Huang, L. Accounts of Chemical Research 2012, 45,971. doi: 10.1021/ar200151m
4. [4]
  (4) Boussif, O.; Lezoualc′h, F.; Zanta, M. A.; Mergny, M. D.;Scherman, D.; Demeneix, B.; Behr, J. P. Proc. Natl. Acad. Sci. U. S. A. 1995, 92, 7297. doi: 10.1073/pnas.92.16.7297
5. [5]
  (5) dbey,W. T.; Barry, M. A.; Saggau, P.;Wu, K. K.; Mikos, A.G. J. Biomed. Mater. Res. 2000, 51, 321.
6. [6]
  (6) Bonner, D. K.; Zhao, X.; Buss, H.; Langer, R.; Hammond, P. T.J. Control. Release 2013, 167, 101. doi: 10.1016/j.jconrel.2012.09.004
7. [7]
  (7) Mahato, M.; Kumar, P.; Sharma, A. K. Molecular Biosystems2013, 9, 780. doi: 10.1039/c3mb25444e
8. [8]
  (8) Patnaik, S.; Gupta, K. C. Expert Opinion on Drug Delivery2013, 10, 215. doi: 10.1517/17425247.2013.744964
9. [9]
  (9) Ogris, M.;Walker, G.; Blessing, T.; Kircheis, R.;Wolschek, M.;Wagner, E. J. Control. Release 2003, 91, 173. doi: 10.1016/S0168-3659(03)00230-X
10. [10]
  (10) Urban-Klein, B.;Werth, S.; Abuharbeid, S.; Czubayko, F.;Aigner, A. Gene Therapy 2005, 12, 461. doi: 10.1038/sj.gt.3302425
11. [11]
  (11) Li, S.;Wang, Y.; Zhang, J.; Yang,W. H.; Dai, Z. H.; Zhu,W.;Yu, X. Q. Mol. Biosyst. 2011, 7, 1254. doi: 10.1039/c0mb00339e
12. [12]
  (12) Kunath, K.; Harpe, A. V.; Fischer, D.; Petersen, H.; Bickel, U.;Voigt, K.; Kissel, T. J. Control. Release 2003, 89, 113. doi: 10.1016/S0168-3659(03)00076-2
13. [13]
  (13) Tsai, L. R.; Chen, M. H.; Chien, C. T.; Chen, M. K.; Lin, F. S.;Lin, K. M. C.; Hwu, Y. K.; Yang, C. S.; Lin, S. Y. Biomaterials2011, 32, 3647. doi: 10.1016/j.biomaterials.2011.01.059
14. [14]
  (14) Chen, J.; Tian, H.; Guo, Z.; Xia, J.; Kano, A.; Maruyama, A.;Jing, X.; Chen, X. Macromol. Biosci. 2009, 9, 1247. doi: 10.1002/mabi.v9:12
15. [15]
  (15) Zhang, L.; Lu, Z.; Zhao, Q.; Huang, J.; Shen, H.; Zhang, Z.Small 2011, 7, 460. doi: 10.1002/smll.201001522
16. [16]
  (16) Werth, S.; Urban-Klein, B.; Dai, L.; Höbel, S.; Grzelinski, M.;Bakowsky, U.; Czubayko, F.; Aigner, A. J. Control. Release2006, 112, 257. doi: 10.1016/j.jconrel.2006.02.009
17. [17]
  (17) Jiang, Q.; Shi, P.; Li, C.;Wang, Q.; Xu, F.; Yang,W.; Tang, G.Macromol. Biosci. 2011, 11, 435. doi: 10.1002/mabi.v11.3
18. [18]
  (18) Roesler, S.; Koch, F. P. V.; Schmehl, T.;Weissmann, N.; Seeger,W.; Gessler, T.; Kissel, T. J. Gene Med. 2011, 13, 123. doi: 10.1002/jgm.v13.2
19. [19]
  (19) Alshamsan, A.; Haddadi, A.; Incani, V.; Samuel, J.; Lavasanifar,A.; Uludag, H. Molecular Pharmaceutics 2009, 6, 121. doi: 10.1021/mp8000815
20. [20]
  (20) Boura, J. S.; Santos, F. D.; Gimble, J. M.; Cardoso, C. M. P.;Madeira, C.; Cabral, J. M. S.; Silva, C. L. D. Human Gene Therapy Methods 2013, 24, 38. doi: 10.1089/hgtb.2012.185
21. [21]
  (21) Morigaki, K.;Walde, P. Current Opinion in Colloid & Interface Science 2007, 12, 75. doi: 10.1016/j.cocis.2007.05.005
22. [22]
  (22) Ferreira, D. A.; Bentley, M. V. L. B.; Karlsson, G.; Edwards, K.Int. J. Pharm. 2006, 310, 203. doi: 10.1016/j.ijpharm.2005.11.028
23. [23]
  (23) Edwards, K.; Silvander, M.; Karlsson, G. Langmuir 1995, 11,2429. doi: 10.1021/la00007a020
24. [24]
  (24) Wang, B.; Rouzier, R.; Albarracin, C. T.; Sahin, A.;Wagner, P.;Yang, Y.; Smith, T. L.; Meric-Bernstam, F.; Marcelo, A. C.;Hortobagyi, G. N.; Pusztai, L. Breast Cancer Research and Treatment 2004, 87, 205. doi: 10.1007/s10549-004-6590-0
25. [25]
  (25) Vader, P.; Aa, L. J. v. d.; Engbersen, J. F. J.; Storm, G.;Schiffelers, R. M. J. Control. Release 2010, 148, 106. doi: 10.1016/j.jconrel.2010.06.019
1. [1]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
2. [2]
  Zhaoxuan ZHU , Lixin WANG , Xiaoning TANG , Long LI , Yan SHI , Jiaojing SHAO . Application of poly(vinyl alcohol) conductive hydrogel electrolytes in zinc ion batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 893-902. doi: 10.11862/CJIC.20240368
3. [3]
  Ying Zhang , Fang Ge , Zhimin Luo . AI-Driven Biochemical Teaching Research: Predicting the Functional Effects of Gene Mutations. University Chemistry, 2025, 40(3): 277-284. doi: 10.12461/PKU.DXHX202412104
4. [4]
  Jiatong Hu , Qiyi Wang , Ruiwen Tang , Jiajing Feng . Photocatalytic Journey of Perylene Diimides in a Competitive Arena. University Chemistry, 2025, 40(5): 328-333. doi: 10.12461/PKU.DXHX202407015
5. [5]
  Chengqian Mao , Yanghan Chen , Haotong Bai , Junru Huang , Junpeng Zhuang . Photodimerization of Styrylpyridinium Salt and Its Application in Silk Screen Printing. University Chemistry, 2024, 39(5): 354-362. doi: 10.3866/PKU.DXHX202312014
6. [6]
  Yi DING , Peiyu LIAO , Jianhua JIA , Mingliang TONG . Structure and photoluminescence modulation of silver(Ⅰ)-tetra(pyridin-4-yl)ethene metal-organic frameworks by substituted benzoates. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 141-148. doi: 10.11862/CJIC.20240393
7. [7]
  Zeyi Yan , Ruitao Liu , Xinyu Qi , Yuxiang Zhang , Lulu Sun , Xiangyuan Li , Anchao Feng . Exploration of Suspension Polymerization: Preparation and Fluorescence Stability of Perovskite Polystyrene Microbeads. University Chemistry, 2025, 40(4): 72-79. doi: 10.12461/PKU.DXHX202405110
8. [8]
  Jiaxin Su , Jiaqi Zhang , Shuming Chai , Yankun Wang , Sibo Wang , Yuanxing Fang . Optimizing Poly(heptazine imide) Photoanodes Using Binary Molten Salt Synthesis for Water Oxidation Reaction. Acta Physico-Chimica Sinica, 2024, 40(12): 2408012-. doi: 10.3866/PKU.WHXB202408012
9. [9]
  Lirui Shen , Kun Liu , Ying Yang , Dongwan Li , Wengui Chang . Synthesis and Application of Decanedioic Acid-N-Hydroxysuccinimide Ester: Exploration of Teaching Reform in Comprehensive Applied Chemistry Experiment. University Chemistry, 2024, 39(8): 212-220. doi: 10.3866/PKU.DXHX202312035
10. [10]
  Feng Zheng , Ruxun Yuan , Xiaogang Wang . “Research-Oriented” Comprehensive Experimental Design in Polymer Chemistry: the Case of Polyimide Aerogels. University Chemistry, 2024, 39(10): 210-218. doi: 10.12461/PKU.DXHX202404027
11. [11]
  Yikai Wang , Xiaolin Jiang , Haoming Song , Nan Wei , Yifan Wang , Xinjun Xu , Cuihong Li , Hao Lu , Yahui Liu , Zhishan Bo . 氰基修饰的苝二酰亚胺衍生物作为膜厚不敏感型阴极界面材料用于高效有机太阳能电池. Acta Physico-Chimica Sinica, 2025, 41(3): 2406007-. doi: 10.3866/PKU.WHXB202406007

Get Citation

PDF

XML

Metrics

PDF Downloads(502)
Abstract views(942)
HTML views(19)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142