Citation: LI Jin-Hua, LI JING, FANG Xuan, WANG Xiao-Hua, WEI Zhi-Peng. Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate[J]. Acta Physico-Chimica Sinica, ;2012, 28(06): 1393-1397. doi: 10.3866/PKU.WHXB201204062 shu

Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

LI Jin-Hua ^1,2, ,
LI JING ¹ ,
FANG Xuan ^1,2 ,
WANG Xiao-Hua ¹ ,
WEI Zhi-Peng ¹

1.
1. School of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China;
2. International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology, Changchun 130022, P. R. China

Received Date: 9 March 2012
Available Online: 6 April 2012
Fund Project: 国家自然科学基金(61006065, 61076039, 10804071) (61006065, 61076039, 10804071) 高等学校博士学科点专项科研基金(20102216110001, 20112216120005) (20102216110001, 20112216120005) 吉林省科技发展计划(20091039, 20090555, 20101546, 20121816, 201201116) (20091039, 20090555, 20101546, 20121816, 201201116)吉林省教育厅项目(JYT2011-05, JYT2011-10, JYT2011-11)资助 (JYT2011-05, JYT2011-10, JYT2011-11)

Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase ( x). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). x was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the x and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and x can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry.
- Glucose oxidase,
- Flexible substrate,
- Biosensor,
- ZnO
1. [1]
  (1) Yu, J.; Ju, H. Anal. Chem. 2002, 74, 3579. doi: 10.1021/ac011290k
2. [2]
  (2) Lee, H.; Yoon, S. W.; Kim, E. J.; Park, J. Nano Lett. 2007, 7, 778. doi: 10.1021/nl0630539
3. [3]
  (3) Vidur, K. L. Melliand-China 2005, 2,75
4. [4]
  (4) Rajesh, C.; Kaneto, K. Curr. Appl. Phys. 2005, 2, 178
5. [5]
  (5) Wang, J.; Fang, L.; Lopez, D. Analyst 1994, 119, 455
6. [6]
  (6) Consnier, S.; Innocent, C. Bioelectrochem. Bioenergy 1993, 3, 147
7. [7]
  (7) Kondo, T.; Horiuchi, S.; Yagi, I.; Ye, S.; Uosaki, K. J. Am. Chem. Soc 121 1999, 121, 391
8. [8]
  (8) Liu, Z. J.; Liu, B. H.; Kong, J. L.; Deng, J. Q. Anal. Chem. 2000, 72, 707
9. [9]
  (9) Wang, B. Q.; Li, B.; Wang, Z. X.; Xu, G. B.; Wang, Q.; Dong, S. J. Anal. Chem 1999, 71,1935
10. [10]
  (10) Muguruma, H.; Hiratsuka, A.; Karube, I. Anal. Chem 2000, 72, 2671
11. [11]
  (11) Yang, Q.; Atanasov, P.; Wilkins, E. Sens. Actuators B: Chem 1998, 46, 249
12. [12]
  (12) Jua, H. Q.; Liua, S. Q.; Geb, B. X.; Lisdatb, F.; Schellerb, F. W. Electroanalysis 2002, 14,141
13. [13]
  (13) Huang, M. H.; Mao, S.; Feick, H.; Yan, H.; Wu, Y.; Kind, H.; Weber, E.; Russo, R.; Yang, P. Science 2001, 292,1897. doi: 10.1126/science.1060367
14. [14]
  (14) Xu, C. X.; Sun, X. W. Appl. Phys. Lett. 2003, 83, 3806
15. [15]
  (15) Gao, P. X.; Ding, Y.; Mai, W.; Hughes, W. L.; Lao, C. S.; Wang, Z. L. Science 2005, 309, 1700
16. [16]
  (16) Sun, X. W.; Kwok, H. S. J. Appl. Phys 1999, 86, 408
17. [17]
  (17) Zhang, F.; Wang, X.; Ai, S.; Sun, Z.; Wan, Q.; Zhu, Z.; Xian, Y.; Jin, L.; Yamamoto, K. Anal. Chim. Acta 2004, 519, 155
18. [18]
  (18) Umar, A.; Rahman, M. M.; Kim, S. H.; Hahn, Y. B. J. Nanosci. Nanotechnol 2008, 8, 3216
19. [19]
  (19) Wei, A. Suna, X. W.; Wang, J. X.; Lei, Y.; Cai, X. P.; Li, C. M.; Dong, Z. L., Huang, W. Appl. Phys. Lett. 2006, 89, 123902
20. [20]
  (20) Liu, Z.; Liu, Y.; Yang, H.; Yang, Y.; Shen, G.; Yu, R. Electroanalysis 2005, 17, 1065
21. [21]
  (21) Cullity, B. D.; Stock, S. R. Elements of X-Ray Diffraction; Prentice Hall: New Jersey, 2001; p 619.
22. [22]
  (22) Topoglidis, E.; Cass, A. E. G.; O'Regan, B.; Durrant, J. R. J. Electroanal. Chem. 2001, 517, 20
23. [23]
  (23) Schvartzman, M.; Sidorov, V.; Ritter, D.; Paz, Y. J. Vac. Sci. Technol. 2001, 169, 68
24. [24]
  (24) Liu, T.; Liao, H.; Lin, C.; Hu, S.; Chen, S. Langmuir 2006, 22, 5804
25. [25]
  (25) Cao, X.; Wang, N.; Li, L.; Guo, L. Sensors and Actuators B 2008, 129, 268
26. [26]
  (26) Chi, Q.; Zhang, J.; Dong, S.; Wang, E. J. Chem. Soc. Faraday. Trans 1994, 90, 2057
27. [27]
  (27) Weber, J.; Jeedigunta, S.; Kumar, A. Journal of Nanomaterials 2008, 1
28. [28]
  (28) Dai, Z.; Liu, K.; Tang, Y.; Yang, X.; Bao, J.; Shen, J. J. Mater. Chem. 2008, 18, 1919
29. [29]
  (29) Kong, T.; Chen, Y.; Ye, Y.; Zhang, K.; Wang, H. Z.; Wang, X. Sens. Actuators B 2009, 38, 344
1. [1]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 2310046-0. doi: 10.3866/PKU.WHXB202310046
2. [2]
  Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101
3. [3]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
4. [4]
  Yu Wang , Shoulei Zhang , Tianming Lv , Yan Su , Xianyu Liu , Fuping Tian , Changgong Meng . Introduce a Comprehensive Inorganic Synthesis Experiment: Synthesis of Nano Zinc Oxide via Microemulsion Using Waste Soybean Oil. University Chemistry, 2024, 39(7): 316-321. doi: 10.3866/PKU.DXHX202311035
5. [5]
  Bin SUN , Heyan JIANG . Glucose-modified bis-Schiff bases: Synthesis and bio-activities in Alzheimer′s disease therapy. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1338-1350. doi: 10.11862/CJIC.20240428
6. [6]
  Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
7. [7]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
8. [8]
  Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
9. [9]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . Photomultiplication-Type All-Polymer Photodetectors and Their Applications in Photoplethysmography Sensor. Acta Physico-Chimica Sinica, 2025, 41(1): 100007-0. doi: 10.3866/PKU.WHXB202311021
10. [10]
  Xiaoning TANG , Shu XIA , Jie LEI , Xingfu YANG , Qiuyang LUO , Junnan LIU , An XUE . Fluorine-doped MnO₂ with oxygen vacancy for stabilizing Zn-ion batteries. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1671-1678. doi: 10.11862/CJIC.20240149
11. [11]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
12. [12]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
13. [13]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
14. [14]
  Yu Dai , Xueting Sun , Haoyu Wu , Naizhu Li , Guoe Cheng , Xiaojin Zhang , Fan Xia . Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide. University Chemistry, 2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052
15. [15]
  Hui-Ying Chen , Hao-Lin Zhu , Pei-Qin Liao , Xiao-Ming Chen . Integration of Ru(Ⅱ)-Bipyridyl and Zinc(Ⅱ)-Porphyrin Moieties in a Metal-Organic Framework for Efficient Overall CO₂ Photoreduction. Acta Physico-Chimica Sinica, 2024, 40(4): 2306046-0. doi: 10.3866/PKU.WHXB202306046
16. [16]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
17. [17]
  Xiaofei Liu , He Wang , Li Tao , Weimin Ren , Xiaobing Lu , Wenzhen Zhang . Electrocarboxylation of Benzylic Phosphates and Phosphinates with Carbon Dioxide. Acta Physico-Chimica Sinica, 2024, 40(9): 2307008-0. doi: 10.3866/PKU.WHXB202307008
18. [18]
  Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
19. [19]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
20. [20]
  Lijun Yue , Siya Liu , Peng Liu . 不同晶相纳米MnO₂的制备及其对生物乙醇选择性氧化催化性能的测试——一个科研转化的综合化学实验. University Chemistry, 2025, 40(8): 225-232. doi: 10.12461/PKU.DXHX202410005

Get Citation

PDF

XML

Metrics

PDF Downloads(816)
Abstract views(2474)
HTML views(60)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142