Citation: ZHANG Zhe, QI Zhi-Mei, ZHANG Rong-Jun. Direct Electrochemistry of Cytochrome c Adlayer on the ITO Electrode[J]. Acta Physico-Chimica Sinica, ;2012, 28(05): 1163-1168. doi: 10.3866/PKU.WHXB201202241 shu

Direct Electrochemistry of Cytochrome c Adlayer on the ITO Electrode

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received Date: 2 December 2011
Available Online: 24 February 2012
Fund Project: 国家重点基础研究发展规划项目(973) (2009CB320300) (973) (2009CB320300) 国家自然科学基金(60978042, 61078039) (60978042, 61078039)

The electrochemical redox reaction of a cytochrome c (Cyt c) adlayer on an indium tin oxide (ITO) electrode was directly monitored and the surface concentrations of Cyt c versus solution concentrations were obtained from cyclic voltammograms. The results indicate that the surface concentration increases from 0.35 × 10^-12 to 1.53 × 10^-12 mol·cm^-2 when the solution concentration is increased from 2 to 10 μmol ·L^-1. A quasi-linear relationship between the reciprocals of surface concentration and solution concentration was observed, indicating that Cyt c adsorption on the ITO electrode closely obeys the Langmuir isothermal adsorption model. The cyclic voltammograms of the Cyt c solutions with the ITO electrode reveal that both adsorbed and dissociated Cyt c molecules are involved in the electrode reaction and that the contribution of dissociated molecules is much larger than that of adsorbed ones. The electrode reaction is basically diffusion controlled and quasi-reversible. Based on the Nicholson method, the average standard heterogeneous rate constant was determined to be 1.65×10^-3 cm· s^-1. The electrochemical activity of the Cyt c adlayer was partially lost when it was kept at 25 °C for 1 h, and was completely lost at 80 °C. The denatured Cyt c adlayer on a ld electrode can effectively inhibit the electrode reaction of K₃Fe(CN)₆ solution.
- Cytochrome c adlayer,
- Direct electrochemistry,
- Langmuir isothermal adsorption,
- Surface concentration
1. [1]
  (1) Eddowes, M. J.; Hill, H. A. O. J. Chem. Soc. Chem. Commun. 1977, No. 21, 771.
2. [2]
  (2) Eddowes, M. J.; Hill, H. A. O. J. Am. Chem. Soc. 1979, 101, 4461.
3. [3]
  (3) Ji, X.; Jin, B. K.; Jin, J.; Nakamura, T. J. Electroanal. Chem. 2006, 590, 173.
4. [4]
  (4) Ding, X. Q.; Yang, M.; Hu, J. B.; Li, Q. L.; McDougall, A. Microchim. Acta 2007, 158, 65.
5. [5]
  (5) Wang, J. L.;Wang, F. A.; Xu, Z. A.;Wang, Y. Z.; Dong, S. J. Talanta 2007, 74, 104.
6. [6]
  (6) Wang, L.;Wang, E. K. Electrochem. Commun. 2004, 6, 49.
7. [7]
  (7) Jiang, X.; Zhang, Z. L.; Bai, H. Y.; Qu, X. H.; Jiang, J. G.; Wang, E. K.; Dong, S. J. Spectrochimica Acta Part A 2005, 61, 943.
8. [8]
  (8) Liang, M. S.; Bai, Y.; Liu, M.; Zheng,W. J. Acta Phys. -Chim. Sin. 2009, 25, 457. [梁敏思, 白燕, 刘敏, 郑文杰. 物理化学学报, 2009, 25, 457.]
9. [9]
  (9) Tai, Y. H.; Zhou, L. H.;Wang, Y.; Ma, L.;Wang, S. T. Journal of Northeast Normal University 2010, 42, 93. [泰玉华, 周立恒, 王岩, 马丽, 王胜天. 东北师大学报, 2010, 42, 93.]
10. [10]
  (10) Wang, Q.; Li, N. Q. Electroanalysis 2001, 13, 1375.
11. [11]
  (11) Ion, A.; Banica, F. J. Solid State Electrochem. 2001, 5, 431.
12. [12]
  (12) Runge, A. F.; Saavedra, S. S. Langmuir 2003, 19, 9418.
13. [13]
  (13) Liu, H. H.; Lu, J. L.; Zhang, M.; Pang, D.W.; Abruna, H. D. J. Electroanal. Chem. 2003, 554, 93.
14. [14]
  (14) McKenzie, K. J.; Marken, F.; Opallo, M. Bioelectrochemistry 2005, 66, 41.
15. [15]
  (15) Zhao, Q.; Zhuang, Q. K. Chin. Chem. Lett. 2005, 16, 1237.
16. [16]
  (16) Ding, X. Q.; Li, J. H.; Hu, J. B.; Li, Q. L. Anal. Biochem. 2005, 339, 46.
17. [17]
  (17) Gu, R. A.; Qiao, Z. H.; Qu, X. G.; Lu, T. H.; Dong, S. J. Acta Phys. -Chim. Sin. 1996, 12, 654. [顾仁敖, 乔专虹, 曲晓刚, 陆天虹, 董绍俊. 物理化学学报, 1996, 12, 654.]
18. [18]
  (18) Qu, X. G.; Sun, G. Q.; Yang, H.; Lu, T. H. Electrochemistry 1998, 4, 260. [曲晓刚, 孙公权, 杨辉, 陆天虹. 电化学, 1998, 4, 260.]
19. [19]
  (19) Li, J. H.; Cheng, G. J.; Dong, S. J. Chemical Research in Chinese University 1998, 14, 17.
20. [20]
  (20) Zhang, R. J.; Yin, Q. F.; Xu, J. M.; Zhu, Y. L.; Ma, K. R. Chemical Reagents 2010, 32, 577. [张瑞娟, 尹起范, 徐继明, 朱玉兰, 马奎荣. 化学试剂, 2010, 32, 577.]
21. [21]
  (21) Xu, J. M.; Zhang, R. J.; Song, J.; Yin, Q. F.; Zhong, H. Chemical Research and Application 2010, 22, 1236. [徐继明, 张瑞娟, 宋洁, 尹起范, 仲慧. 化学研究与应用, 2010, 22, 1236.]
22. [22]
  (22) Wang, Y.; Qian, K.; Guo, K.; Kong, J. L.; Marty, J. L.; Yu, C. Z.; Liu, B. H. Microchim. Acta 2011, 175, 87.
23. [23]
  (23) Kasmi, A. E.; Leopold, M. C.; Galligan, R.; Robertson, R. T.; Saavedra, S. S.; Kacami, K. E.; Bowden, E. F. Electrochem. Commun. 2002, 4, 177.
24. [24]
  (24) Shie, J.W.; Yogeswaran, U.; Chen, S. M. Talanta 2008, 74, 1659.
25. [25]
  (25) Matsuda, N.; Santos, J. H.; Takatsu, A.; Kato, K. Thin Solid Films 2003, 438-439, 403.
26. [26]
  (26) Li, S. Q.; Xia, J.; Liu, C. Y.; Cao,W.; Hu, J. B.; Li, Q. L. J. Electroanal. Chem. 2009, 633, 273.
27. [27]
  (27) Yan, J.; Li, J. J.; Zhang, B.; Cai, S. M. Acta Phys. -Chim. Sin. 2001, 17, 1126. [严捷, 李经建, 张波, 蔡生民. 物理化学学报, 2001, 17, 1126.]
28. [28]
  (28) Renault, C.; Harris, K. D.; Brett, M. J. Chem. Commun. 2011, 47, 1863.
29. [29]
  (29) Lee, I.; Lee, S. Y. J. Phys. Chem. C 2009, 113, 17372.
30. [30]
  (30) Laviron, E. J. Electroanal. Chem. 1979, 100, 263.
31. [31]
  (31) Bard, A. J.; Faulkner, L. R. Electrochemical Methods: Fundamentals and Applications, 2nd ed.; JohnWiley & Sons, Inc.: Hoboken, 2001; pp 231-232, 239-243, 590-595.
32. [32]
  (32) Nicholson, R. S. Anal. Chem. 1965, 37, 1351.
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沈阳化工大学材料科学与工程学院沈阳 110142