Citation: LI Na, ZHOU Jian-Zhang, LIN Ling-Ling, HAN Nan-Nan, LIN Zhong-Hua. Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c[J]. Acta Physico-Chimica Sinica, ;2010, 26(09): 2468-2472. doi: 10.3866/PKU.WHXB20100923 shu

Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c

1.
Key Laboratory of Physical Chemistry of Solid Surface, Department of Chemistry, College of Chemistry and Chemistry Engineering, Xiamen University, Xiamen 361005, Fujian Province, P. R. China

Received Date: 8 March 2010
Available Online: 16 July 2010
Fund Project: 国家自然科学基金(20603027, 20423002)资助项目 (20603027, 20423002)

The colors of Au nanoparticles (Au NPs) change along with conformational changes in cytochrome c (Cyt c). We exploited this property for the colorimetric detection of Cyt c conformational changes induced by H⁺ and L-cysteine (L-Cys). We improved the conventional procedure for this detection. After the addition of Cyt c within different pH values, the Au NPs are either cyan, blue, purple or red. This indicates that the Au NPs can be applied to the rapid colorimetric detection of pH-induced conformational changes in Cyt c. At pH 7 the color of Au NPs changes from purple to blue and then cyan upon the addition of L-Cys, which suggests that the Au NPs can be used for the colorimetric detection of Cyt c conformational changes caused by interaction with L-Cys. The conformational changes of Cyt c were verified by circular dichroism (CD) spectroscopy. The relationship between the aggregation states and colors of the Au NPs after the addition of Cyt c was characterized by UV-Vis absorption spectroscopy and scanning electron microscopy (SEM).
- Au nanoparticle,
- Colorimetric detection,
- Conformational changes of Cyt c,
- pH value,
- L-Cys
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