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Citation: LI Tao, LV Rong, YU An-Chi. Recombination Process between Myoglobin and NO Studied by Time-Resolved Raman Spectroscopy[J]. Acta Physico-Chimica Sinica, ;2010, 26(01): 18-22. doi: 10.3866/PKU.WHXB20100119 shu

Recombination Process between Myoglobin and NO Studied by Time-Resolved Raman Spectroscopy

1.
Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China

Received Date: 14 September 2009
Available Online: 25 November 2009

Nanosecond transient Raman spectroscopy is a widely used technique to investigate ultrafast structural dynamics in molecules. We studied the rebinding kinetics between deoxymyoglobin (DeoxyMb) and NO using nanosecond transient Raman spectroscopy. By monitoring the ratio between the intensity of the υ4 vibrational mode in the photoproduct (DeoxyMb) and in the reactant (MbNO) at different incident laser fluxes, we obtained the recombination kinetics between DeoxyMb and NO in MbNO. For comparison, the kinetics of NO rebinding to DeoxyMb in MbNO was also studied using picosecond time-resolved Raman and absorption experiments. The kinetics results obtained with nanosecond transient Raman were consistent with those obtained by picosecond time-resolved Raman and absorption experiments.
- Nanosecond transient Raman,
- Picosecond time-resolved Raman,
- Picosecond time-resolved absorption,
- Myoglobin,
- NO,
- Recombination
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