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Citation: FANG Ying-Ying, HU Xin-Gen, YU Li, LI Wen-Bing, LIN Rui-Sen. Study on Thermal Denaturation of Lysozyme by DSC[J]. Acta Physico-Chimica Sinica, ;2007, 23(01): 84-87. doi: 10.3866/PKU.WHXB20070117 shu

Study on Thermal Denaturation of Lysozyme by DSC

1.
School of Chemistry and Materials Science, Wenzhou University, Wenzhou 325027, Zhejiang Province, P. R. China; Key Laboratory for Colloid and Interface Chemistry of the Ministry of Education, Shandong University, Jinan 250100, P. R. China; Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China

Received Date: 2 June 2006
Available Online: 8 January 2007

The thermal denaturation of solid lysozyme and the effects of denaturants and their concentrations on denaturation of lysozyme in aqueous solutions were studied by differential scanning calorimetry (DSC). The results showed that both denaturation temperature and denaturation enthalpy of lysozyme decreased as the existence of water and the addition of urea and guanidine hydrochloride (GuHCl). In addition, denaturation temperature and denaturation enthalpy decreased as the concentrations of urea and GuHCl increased. As a denaturant, GuHCl is more effective than urea due to its added ability of electrostatic interaction.
- Lysozyme;Urea;GuHCl;DSC;Thermal denaturation
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