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Citation: Zhou Xiao-Lei, Wang Bao-Huai, Li Zhi-Fen, Zeng Hui-Hui. Studies of the Effects of Anti-tumor Compounds on the Assembly and Melting Denaturation of Tubulin in vitro[J]. Acta Physico-Chimica Sinica, ;2002, 18(11): 1009-1013. doi: 10.3866/PKU.WHXB20021110 shu

Studies of the Effects of Anti-tumor Compounds on the Assembly and Melting Denaturation of Tubulin in vitro

1.
Institute of Physical Chemistry, Peking University, Beijing　100871;School of Pharmaceutical Science, Peking University, Beijing,　100083

Received Date: 28 March 2002
Available Online: 15 November 2002

The effects of four kinds of anti-tumor compounds, taxol, carboplatin, ZYZ (the extractive from gleditsia), F43 (the extractive from squamocin), on the assembly and melting denaturation of tubulin in vitro were studied by turbidity and differential scanning calorimetry. The experimental results show that taxol and ZYZ enhance the assembly of tubulin, while carboplatin and F43 have no effect on the assembly of tubulin when the concentrations of all four kinds of compounds are 15 μmol•L－1.At the same time, taxol and ZYZ can make the melting points of tubuin rise, while the melting points of tubuin don't change in the presence of carboplatin and F43.The different interaction mechanisms of the compounds and tubulin have been discussed according to the above experimental results.
- Tubulin;Taxol;Carboplatin;ZYZ;F43;Turbidity;DSC(differential scanning calorimetry)
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