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Citation: Rong Tian, Yadi Yang, Naihao Lu. Comprehensive Experimental Design of Undergraduate Students Based on Interdisciplinarity: Study on the Effect of Quercetin on Chlorination Activity of Myeloperoxidase[J]. University Chemistry, ;2024, 39(8): 247-254. doi: 10.3866/PKU.DXHX202312064 shu

Comprehensive Experimental Design of Undergraduate Students Based on Interdisciplinarity: Study on the Effect of Quercetin on Chlorination Activity of Myeloperoxidase

Rong Tian ^1,2 ,
Yadi Yang ^1,2 ,
Naihao Lu ^1,2

1.
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China;
2.
National Demonstration Center for Experimental Chemistry Education (Jiangxi Normal University), Nanchang 330022, China

Received Date: 19 December 2023
Revised Date: 20 February 2024

This paper presents a comprehensive experiment within the interdisciplinary field of chemical biology, closely aligned with the cutting-edge of the discipline, deeply integrating science and education. Utilizing both chemical and biological methods within an in vitro biochemical reaction system, this study investigates effects of the natural antioxidant quercetin on the redox cycle of myeloperoxidase (MPO) within biological organisms. The mechanism of quercetin affecting the chlorination activity of MPO is clarified through molecular docking techiniques and a series of UV-Vis absorption spectroscopy. The implementation of this experiment aids in broadening students’ perspectives, igniting their interest in scientific research, and enhancing their comprehensive experimental abilities. It offers valuable insights and examples for the development of multidisciplinary innovative talents in chemistry-related majors.
- Interdisciplinary,
- Quercetin,
- Myeloperoxidase,
- Chlorination activity,
- Comprehensive experiment
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
  Nie, J.; Yu, C.; Guo, Y.; Pei, P.; Chen, L.; Pang, Y.; Du, H.; Yang, L.; Chen, Y.; Yan, S.; et al. Am. J. Clin. Nutr. 2021, 114 (1), 194.
7. [7]
  Ulusoy, H. G.; Sanlier, N. Crit. Rev. Food Sci. Nutr. 2020, 60 (19), 3290.
8. [8]
  Ramachandra, C. J. A.; Ja, K. P. M. M.; Chua, J.; Cong, S.; Shim, W.; Hausenloy, D. J. Antioxid. Redox Signal. 2020, 32 (15), 1135.
9. [9]
  Tian, R.; Jin, Z.; Zhou, L.; Zeng, X. P.; Lu, N. J. Agric. Food Chem. 2021, 69 (1), 404.
10. [10]
  Lu, N.; Sui, Y.; Tian, R.; Peng, Y. Y. J. Agric. Food Chem. 2018, 66 (19), 4933.
11. [11]
1. [1]
  Luhong Chen , Yan Zhang . Chem&Bio Interdisciplinary Graduates Training in Nanjing University Promoted by Chemistry and Biomedicine Innovation Center. University Chemistry, 2024, 39(6): 12-16. doi: 10.3866/PKU.DXHX202311089
2. [2]
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3. [3]
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7. [7]
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9. [9]
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11. [11]
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12. [12]
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13. [13]
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14. [14]
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15. [15]
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17. [17]
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19. [19]
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20. [20]
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