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Citation: Qin Tu, Anju Tao, Tongtong Ma, Jinyi Wang. Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip[J]. University Chemistry, ;2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062 shu

Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip

Qin Tu ¹ ,
Anju Tao ¹ ,
Tongtong Ma ^1,2 ,
Jinyi Wang ¹

1.
College of Chemistry & Pharmacy, Northwest A & F University, Yangling 712100, Shaanxi Province, China;
2.
Chuxiong Normal University, Chuxiong 675099, Yunnan Province, China

Received Date: 18 September 2023
Revised Date: 10 January 2024

An innovative experimental approach based on a microfluidic paper-based chip has been designed for specific detection of Escherichia coli (E. coli). The chip is fabricated for detecting E. coli in water samples. The main contents include the preparation of paper chips, lysis of E. coli on the chip, detection of E. coli, optimization of detection conditions, and detection of E. coli in actual samples. This experiment integrates cutting-edge disciplines, demonstrating robust comprehensive and innovative characteristics. It aligns with the practical education concept marked by the progression from “basic experiment → comprehensive experiment → research-oriented experiment”, effectively strengthening students’ capacities for problem analysis and solution.
- E. coli,
- Paper-based chip,
- Visual and quantitative detection,
- Innovative experimental
1. [1]
  Shaibani, P.M.; Jiang, K.; Haghighat, G.; Hassanpourfard, M.; Etayash, H.; Naicker, S.;Thundat, T. Sensor. Actuat. B-Chem. 2016, 226, 176.
2. [2]
3. [3]
  Hossain, S.M. Z.; Brennan, J. D. Anal. Chem. 2011, 83(22), 8772.
1. [1]
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