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Citation: Shuangyan Huan, Ke Li, Xiaobing Zhang, Weiping Luo, Yuzhi Wang, Xia Yin, Tianbing Ren, Hongxia Gao, Zhiwu Liang. Virtual Simulation Experiment for the Design and Exploration of Tumor in Situ Fluorescence Labeling Technology[J]. University Chemistry, ;2023, 38(11): 171-179. doi: 10.3866/PKU.DXHX202303056 shu

Virtual Simulation Experiment for the Design and Exploration of Tumor in Situ Fluorescence Labeling Technology

1.
College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
2.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Corresponding author: Shuangyan Huan, syhuan@hnu.edu.cn
Received Date: 16 March 2023

In situ fluorescence imaging technology offers an efficacious method for garnering biochemical information at cellular and organismal scales. Prolonged in situ labeling techniques, using solid-state fluorescent probes, are particularly suited for precise imaging and surgical navigation during tumor resections. However, the inherent challenges, including extended research cycles, high costs, and substantial resource utilization, often preclude their inclusion in undergraduate teaching. To surmount these obstacles, we transformed the latest research findings into a "virtual simulation experiment of tumor in situ fluorescent labeling technology design and exploration" utilizing the U3D three-dimensional virtual reality technology platform. Through an interactive 37-step operation and exploratory teaching approach centred on crucial scientific issues, we aim to hone students' practical skills, foster scientific thinking and innovative awareness, actively guide the development of their scientific literacy, and instil values pertinent to scientific research.
- In situ fluorescence imaging,
- Virtual simulation experiment,
- Fluorescent probes,
- Surgical navigation
1. [1]
  Ntziachristos, V. Annu. Rev. Biomed. Eng. 2006, 8, 1.
2. [2]
  Yang, Y.; Zhao, Q.; Feng, W.; Li, F. Chem. Rev. 2013, 113, 192.
3. [3]
  Landau, M. J.; Gould, D. J.; Patel, K. M. Ann. Transl. Med. 2016, 4 (20), 392.
4. [4]
5. [5]
  Li, X.; Gao, X.; Shi, W.; Ma, H. Chem. Rev. 2014, 114 (1), 590.
6. [6]
  Devaraj, N. K.; Upadhyay, R.; Haun, J. B.; Hilderbrand, S. A.; Weissleder, R. Angew. Chem. Int. Ed. 2009, 48 (38), 7013.
7. [7]
  Li, K.; Lyu, Y.; Huang, Y.; Xu, S.; Liu, H.; Chen, L.; Ren, T.; Xiong, M.; Huan, S.; Yuan, L.; et al. Proc. Natl. Acad. Sci. USA 2021, 118 (8), e2018033118.
8. [8]
  ]. http://www.moe.gov.cn/srcsite/A08/s7945/s7946/201707/t20170721_309819.html
1. [1]
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