Citation: Zipin Zhang, Zuzhi Zhao. An Indicator Displacement Assay (IDA)-based Analytical Method for Heparin Detection: A Comprehensive Experiment in Analytical Chemistry[J]. University Chemistry, ;2023, 38(8): 240-247. doi: 10.3866/PKU.DXHX202212017 shu

An Indicator Displacement Assay (IDA)-based Analytical Method for Heparin Detection: A Comprehensive Experiment in Analytical Chemistry

Zipin Zhang ^1,, ,
Zuzhi Zhao ²

1.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;
2.
School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China

Corresponding author: Zipin Zhang, zpzhang@ahtcm.edu.cn
Received Date: 5 December 2022

Herein we describe a comprehensive experiment designed for laboratory teaching of analytical chemistry. The experiment was developed based on the results of one of our recent studies. Methyl viologen (MV²⁺) and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (pyranine) were used as the heparin receptor and optical indicator, respectively. Once heparin was introduced in the reaction system, competitive binding led to pyranine displacement, thereby resulting in changes in the UV-Vis absorbance of the pyranine/MV²⁺ system. Using the changes in UV-Vis absorption as the output signal, heparin concentration can be accurately measured using the IDA-based method.
- Analytical chemistry,
- Comprehensive design experiment,
- Indicator displacement assay (IDA),
- Heparin
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