RGB阵列式荧光传感技术用于检测高原湖泊中多种重金属离子

引用本文: 马璐瑶, 张钧富, 陈怡橦, 杨通. RGB阵列式荧光传感技术用于检测高原湖泊中多种重金属离子[J]. 大学化学, 2023, 38(4): 78-87. doi: 10.3866/PKU.DXHX202211048 shu

Citation: Luyao Ma, Junfu Zhang, Yitong Chen, Tong Yang. RGB Array Fluorescent Sensing Technology for the Detection of Multiple Heavy Metal Ions in Plateau Lakes[J]. University Chemistry, 2023, 38(4): 78-87. doi: 10.3866/PKU.DXHX202211048 shu

RGB阵列式荧光传感技术用于检测高原湖泊中多种重金属离子

云南师范大学化学化工学院, 昆明 650500

通讯作者: 杨通, yangtong2018@ynnu.edu.cn

基金项目:
国家自然科学基金项目(21904114)；云南省基础研究计划项目(202001AU070067,202201AT070028)

摘要: 众所周知，重金属离子难在自然界中降解，一定量的重金属离子会在生物体中发生富集，严重威胁生命体健康。云南省拥有丰富的高原湖泊淡水资源，开展对高原湖泊重金属污染的研究，有助于深入了解高原湖泊重金属污染的现状，为其治理提供依据。荧光探针在重金属离子检测方面具有灵敏度高、选择性好、操作简单等优点。本新创实验将合成三种不同荧光发射的纳米材料：碳点(CDs，蓝色)、量子点(CdTe QDs，绿色)、金纳米簇(Au NCs，红色)。在96孔板中，分别加入以上三种荧光纳米探针以及CDs和Au NCs的混合式比率型探针，形成5 × 12的阵列形式，通过荧光猝灭及比率荧光的方式实现对高原湖泊水样中Pb²⁺、Cu²⁺、Hg²⁺的检测。本实验还在紫外灯下，借助智能手机APP——Colorimeter X读取溶液的R、G、B值，基于此可实现金属离子的便捷式检测。本新创实验仪器小型化、高通量检测、视觉效果好，在应用于高校综合性实验开展方面，可解决部分高校实验仪器短缺、本科实验枯燥、与科研前沿相脱轨的问题。

关键词:

English

RGB Array Fluorescent Sensing Technology for the Detection of Multiple Heavy Metal Ions in Plateau Lakes

College of Chemical and Chemical Engineering, Yunnan Normal University, Kunming 650500, China

Corresponding author: Tong Yang, yangtong2018@ynnu.edu.cn

Received Date: 15 November 2022

Abstract: As we all know, heavy metal ions are difficult to degrade in nature, and a certain amount of heavy metal ions will be enriched in living organisms, which seriously threatens the health of life. Yunnan Province has abundant freshwater resources in plateau lakes. The research on heavy metal pollution in plateau lakes is conducive to in-depth understanding of the current situation of heavy metal pollution in plateau lakes, which can provide a basis for its comprehensive control. Fluorescent nanoprobes exhibit advantages such as high sensitivity, good selectivity and simple operation in the detection of heavy metal ions. Three nanomaterials with different fluorescence-emission including carbon dots (CDs, blue), quantum dots (CdTe QDs, green), and gold nanoclusters (Au NCs, red) were successfully synthesized. Then, the above three fluorescent nanoprobes and the mixed ratiometric probes of CDs and Au NCs were added into the 96-well plate to form a 5 × 12 array which was used to detect Pb²⁺, Cu²⁺ and Hg²⁺ in the plateau lake water sample based on the fluorescence quenching and ratiometric fluorescence. In addition, the RGB values of the above solution in 96-well plate were recorded by APP-Colorimeter X in smartphone under the ultraviolet lamp. This proposed method exhibited superior testing performances toward Pb²⁺, Cu²⁺ and Hg²⁺, including portability, high-throughout, sensitivity, good visual effect, miniaturization, and small volume. Finally, this newly created experiment can solve the shortage problems of experimental instruments in some universities, boring undergraduate experiments, and derailment with the frontier of scientific research, which can facilitate the development of comprehensive experiments in universities.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

RGB阵列式荧光传感技术用于检测高原湖泊中多种重金属离子

通讯作者: 杨通, yangtong2018@ynnu.edu.cn

English

RGB Array Fluorescent Sensing Technology for the Detection of Multiple Heavy Metal Ions in Plateau Lakes

Corresponding author: Tong Yang, yangtong2018@ynnu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

RGB阵列式荧光传感技术用于检测高原湖泊中多种重金属离子

通讯作者: 杨通, yangtong2018@ynnu.edu.cn

English

RGB Array Fluorescent Sensing Technology for the Detection of Multiple Heavy Metal Ions in Plateau Lakes

Corresponding author: Tong Yang, yangtong2018@ynnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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