Citation: Hong Du, Mi Zhang, Han Zeng. Comprehensive Experiment of Evaluating the Photoelectrocatalytic Eerformance of the Enzyme Nanocomposite: Reform and Practice of Multidisciplinary Comprehensive Experiment[J]. University Chemistry, ;2023, 38(5): 234-240. doi: 10.3866/PKU.DXHX202207059 shu

Comprehensive Experiment of Evaluating the Photoelectrocatalytic Eerformance of the Enzyme Nanocomposite: Reform and Practice of Multidisciplinary Comprehensive Experiment

College of Chemistry and Chemical Engineering, Xinjiang Normal Univeresity, Urmiq 830054, China

Corresponding author: Han Zeng, zenghan1289@163.com
Received Date: 13 July 2022

Regarding the current photo-electrochemical sensor on the basis of TiO₂-carbon nano-material composite with enzyme incorporation, defects such as unfavorable environmental adaptability, undesirable anti-interference capability, inferior sensitivity, unsatisfactory substrate selectivity and insufficient efforts on the constitution-function relationship study are identified. The dependence of the interactions between the variable functional units within nano-complex and TiO₂/carbon nano-material on the structure, composition as well as physicochemical properties of the elements in nano-composite is systematically discussed. Efforts are made to define the key factor to restrict the sensing efficiency and applications of as-prepared photo-electrochemical sensor. The critical issue for the constructing the constitution-performance relationship model to develop the next generation photo-electrochemical sensor is elucidated. A classical comprehensive experiment is designed and presented. The method for preparing the electrode on the basis of nano-composite via the covalent coupling of TiO₂ nanosphere with 3D graphene oxide, and hemoglobin incorporation is introduced. The implementation of this experiment is aimed at the training of the students to use multiple techniques. Such characterization and measurement techniques can be proposed to explore the influence of the interactions between elements in nano-complex and incorporated enzyme molecules on the dynamics of relevant steps involved in the enzyme based photo-electrocatalytic reaction. The experimental set up is proposed to make the students understand and master the interrelated theory and means to determine the kinetic parameters of photo-electrocatalysis for enzyme-based electrode. The idea of closely integrating theory with practice, rigorous and realistic scientific attitude for the educational object could be developed with the execution of the comprehensive experiment.
- Titanium dioxide,
- Carbon nano-materials,
- Photo-electrochemical sensor,
- Comprehensive experiment,
- Catalytic dynamics
1. [1]
  Reyes-De-Corcuera, J. I.; Olstad, H. E.; Garcia-Torres, R. Annu. Rev. Food Sci. Technol. 2018, 9, 293.
2. [2]
3. [3]
  Hu, M. Y.; Li, Z. Z.; Guo, C. P.; Wang, M. H.; He, L. H.; Zhang, Z. H. Appl. Surf. Sci. 2019, 489, 13.
4. [4]
  Wang, M. H.; Zhang, H.; Liu, M. L.; Liu, Y. Biosens. Bioelectron. 2021, 171, 112730.
5. [5]
  Wei, J.; Aori, Q. L.; Yan, Y.; Lei, H. T.; Zhang, S. S.; Liu, W. P.; Liu, Y. J. Anal. Chim. Acta 2021, 93 (34), 11816.
6. [6]
7. [7]
  Zhang, S. W.; Zhang, M.; Wang, F.; Zeng, H. Chem. Eng. J. 2022, 430, 132619.
8. [8]
  ]. https://3g.163.com/dy/article/GROU57BE0516RJ0M.html
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
  Kamaljeet, B. S.; SenGupta, U. Frontiers in Chem. 2017, 4, 50.
16. [16]
17. [17]
  Shleev, S.; Christenson, A.; Serezhenkov, V.; Burbaev, D.; Yaropolov, A.; Gorton, L.; Ruzgas, T. Biochem. J. 2005, 385, 745.
18. [18]
19. [19]
  Zhao, H. Y.; Zhou, H. M.; Zhang, J. X.; Zheng, W.; Zheng, Y. F. Biosens. Bioelectron. 2009, 25 (2), 463.
20. [20]
  Qiu, H. J.; Xu, C. X.; Huang, X. R.; Ding, Y.; Qu, Y. B.; Gao, P. J. J. Phys. Chem. C 2008, 112 (38), 14781.
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
1. [1]
  Xiufang Wang , Donglin Zhao , Kehua Zhang , Xiaojie Song . “Preparation of Carbon Nanotube/SnS₂ Photoanode Materials”: A Comprehensive University Chemistry Experiment. University Chemistry, 2024, 39(4): 157-162. doi: 10.3866/PKU.DXHX202308025
2. [2]
  Xinyan Chen , Meng Xiao , Fei Cai , Junxian Guo , Tianfeng Chen , Li Ma . Transformation of Scientific Research Achievements Facilitating the Construction of Experimental Courses in Frontier Interdisciplinary Disciplines: A Case of “Comprehensive Experiments in Chemical Biology”. University Chemistry, 2025, 40(7): 373-379. doi: 10.12461/PKU.DXHX202408105
3. [3]
  Laiying Zhang , Yinghuan Wu , Yazi Yu , Yecheng Xu , Haojie Zhang , Weitai Wu . Innovation and Practice of Polymer Chemistry Experiment Teaching for Non-Polymer Major Students of Chemistry: Taking the Synthesis, Solution Property, Optical Performance and Application of Thermo-Sensitive Polymers as an Example. University Chemistry, 2024, 39(4): 213-220. doi: 10.3866/PKU.DXHX202310126
4. [4]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
5. [5]
  Kexin Feng , Jie Zhang , Yujia Sun , Qiong Ai , Longchun Li . 乙酰二茂铁和二茂铁甲酰丙酮的合成、纯化及表征. University Chemistry, 2025, 40(8): 307-314. doi: 10.12461/PKU.DXHX202409045
6. [6]
  Shengjuan Huo , Xiaoyan Zhang , Xiangheng Li , Xiangning Li , Tianfang Chen , Yuting Shen . Unveiling the Marvels of Titanium: Popularizing Multifunctional Colored Titanium Product Films. University Chemistry, 2024, 39(5): 184-192. doi: 10.3866/PKU.DXHX202310127
7. [7]
  Ruiqing LIU , Wenxiu LIU , Kun XIE , Yiran LIU , Hui CHENG , Xiaoyu WANG , Chenxu TIAN , Xiujing LIN , Xiaomiao FENG . Three-dimensional porous titanium nitride as a highly efficient sulfur host. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 867-876. doi: 10.11862/CJIC.20230441
8. [8]
  Wei Li , Ze Chang , Meihui Yu , Ying Zhang . Curriculum Ideological and Political Design of Piezoelectricity Measurement Experiments of Coordination Compounds. University Chemistry, 2024, 39(2): 77-82. doi: 10.3866/PKU.DXHX202308004
9. [9]
  Meng Lin , Hanrui Chen , Congcong Xu . Preparation and Study of Photo-Enhanced Electrocatalytic Oxygen Evolution Performance of ZIF-67/Copper(I) Oxide Composite: A Recommended Comprehensive Physical Chemistry Experiment. University Chemistry, 2024, 39(4): 163-168. doi: 10.3866/PKU.DXHX202308117
10. [10]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
11. [11]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 2310046-0. doi: 10.3866/PKU.WHXB202310046
12. [12]
  Kai Yang , Gehua Bi , Yong Zhang , Delin Jin , Ziwei Xu , Qian Wang , Lingbao Xing . Comprehensive Polymer Chemistry Experiment Design: Preparation and Characterization of Rigid Polyurethane Foam Materials. University Chemistry, 2024, 39(4): 206-212. doi: 10.3866/PKU.DXHX202308045
13. [13]
  Yan Li , Xinze Wang , Xue Yao , Shouyun Yu . 基于激发态手性铜催化的烯烃E→Z异构的动力学拆分——推荐一个本科生综合化学实验. University Chemistry, 2024, 39(5): 1-10. doi: 10.3866/PKU.DXHX202309053
14. [14]
  Yue Wu , Jun Li , Bo Zhang , Yan Yang , Haibo Li , Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028
15. [15]
  Jinyi Sun , Lin Ma , Yanjie Xi , Jing Wang . Preparation and Electrocatalytic Nitrogen Reduction Performance Study of Vanadium Nitride@Nitrogen-Doped Carbon Composite Nanomaterials: A Recommended Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(4): 184-191. doi: 10.3866/PKU.DXHX202310094
16. [16]
  Shuhui Li , Rongxiuyuan Huang , Yingming Pan . Electrochemical Synthesis of 2,5-Diphenyl-1,3,4-Oxadiazole: A Recommended Comprehensive Organic Chemistry Experiment. University Chemistry, 2025, 40(5): 357-365. doi: 10.12461/PKU.DXHX202407028
17. [17]
  Caixia Lin , Zhaojiang Shi , Yi Yu , Jianfeng Yan , Keyin Ye , Yaofeng Yuan . Ideological and Political Design for the Electrochemical Synthesis of Benzoxathiazine Dioxide Experiment. University Chemistry, 2024, 39(2): 61-66. doi: 10.3866/PKU.DXHX202309005
18. [18]
  Qiang Zhang , Yuanbiao Huang , Rong Cao . Imidazolium-Based Materials for CO₂ Electroreduction. Acta Physico-Chimica Sinica, 2024, 40(4): 2306040-0. doi: 10.3866/PKU.WHXB202306040
19. [19]
  Jichao XU , Ming HU , Xichang CHEN , Chunhui WANG , Leichen WANG , Lingyi ZHOU , Xing HE , Xiamin CHENG , Su JING . Construction and hydrogen peroxide-activated chemodynamic activity of ferrocene?benzoselenadiazole conjugate. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1495-1504. doi: 10.11862/CJIC.20250144
20. [20]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044

Get Citation

PDF

XML

Metrics

PDF Downloads(11)
Abstract views(1922)
HTML views(104)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142