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Citation: Tianyao Xie, Ailing Deng, Hua Xiao, Fang Zhu. Study on the Influence of the Substitution Degree of Hydroxypropyl-β-Cyclodextrin in Chiral Capillary Electrophoresis Experiments[J]. University Chemistry, ;2023, 38(1): 187-191. doi: 10.3866/PKU.DXHX202202025 shu

Study on the Influence of the Substitution Degree of Hydroxypropyl-β-Cyclodextrin in Chiral Capillary Electrophoresis Experiments

School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China

Corresponding author: Tianyao Xie, cesxty@mail.sysu.edu.cn
Received Date: 12 February 2022

Because of the important influence of the chiral selector substitution degree on the enantioseparation of ofloxacin in chiral capillary electrophoresis experiments, the effects of the substitution degree of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the inclusion complex between ofloxacin and HP-β-CD were investigated according to different substitution degrees. The results showed that the enantioseparation resolution changed significantly with the substitution degree. Moreover, the best separation resolution was found for a DS (degree of substitution) = 7.9. The methods of determining DS were also discussed. Using these methods, the quality of commercial hydroxypropyl-β-cyclodextrin products can be effectively monitored, and the stability and effectiveness in teaching of the chiral capillary electrophoresis experiment can be ensured.
- Chiral capillary electrophoresis experiment,
- Substitution degree,
- Chiral drugs analysis,
- Hydroxypropyl-β-cyclodextrin,
- Ofloxacin,
- Instrumental analysis experiment
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