Abstract: Facing the demand of strengthening the collaborative innovation ability of postgraduates under the background of emerging engineering education, the authors based on many years of teaching and scientific research experience carry out a series of teaching reforms on the teaching content, teaching methods, teaching means and assessment methods for the advanced separation engineering course. The project case base is established, case teaching and team cooperation are adopted to integrate multi-field professional knowledge and the latest scientific research achievements. Professional software is used to solve complex engineering problems, and the manual calculation and software calculation exercises are combined to strengthen the understanding and application of theoretical knowledge. The diversified assessment mode is taken to improve the study enthusiasm and autonomous learning ability. The practice shows that the teaching reforms and implementation of this course obviously improve the teaching effect, which is of great significance to the cultivation of postgraduates' comprehensive engineering ability, team communication and innovative thinking ability.