Citation: Lingling Feng, Qiong Chen, Yumeng Li, Jinghan Liang, Zhou Wang. Preparation of an Important Enzyme FBP/SBPase from Cyanobacteria and Analysis of Interaction between Target Enzyme and Ligand: One Comprehensive Experiment Based on Research[J]. University Chemistry, ;2021, 36(6): 200608. doi: 10.3866/PKU.DXHX202006087 shu

Preparation of an Important Enzyme FBP/SBPase from Cyanobacteria and Analysis of Interaction between Target Enzyme and Ligand: One Comprehensive Experiment Based on Research

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, China

Corresponding author: Lingling Feng, fll708@mail.ccnu.edu.cn
Received Date: 29 June 2020

Cyanobacteria fructose-1,6-/sedoheptanose-1,7-diphosphatase (Cy-FBP/SBPase) was used as the target enzyme, which was obtained through heterologous expression and purification. The purity and content of the target enzyme and the interaction between the enzyme and its ligands (substrate, metal ion and inhibitor) were analyzed. Through the experimental study and exploration, students can master the biosynthesis method to obtain the target enzyme, master the method to analyze the interaction between the target enzyme and its ligand, understand research ideas and experimental techniques needed to establish a drug screening model based on the target enzyme, upgrade the abilities to analyze and solve problems and use chemical biology knowledge, strengthen the social consciousness of scientific research service and social responsibility.
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