以亮氨酸合成为例刍议代谢中的生物化学相似性规律

引用本文: 蒋振雄, 王鹏, 马新雨, 田杰, 张军, 姜汉杰, 石瑾慧, 王志鹏. 以亮氨酸合成为例刍议代谢中的生物化学相似性规律[J]. 大学化学, 2019, 34(5): 80-87. doi: 10.3866/PKU.DXHX201808004 shu

Citation: JIANG Zhenxiong, WANG Peng, MA Xinyu, TIAN Jie, ZHANG Jun, JIANG Hanjie, SHI Jinhui, WANG Zhipeng A.. Discussion on the Biochemical Similarity in Metabolic Principles Based on Leucine Biosynthesis Pathway[J]. University Chemistry, 2019, 34(5): 80-87. doi: 10.3866/PKU.DXHX201808004 shu

以亮氨酸合成为例刍议代谢中的生物化学相似性规律

蒋振雄 ^1, ,
王鹏 ^2, ,
马新雨 ^3, ,
田杰 ^1, ,
张军 ^5, ,
姜汉杰 ^4, ,
石瑾慧 ^6, ,
王志鹏 ^1,3,4,

1.
Department of Biology, Texas A&M University, College Station, TX 77840, USA;
2.
中山大学附属第一医院烧伤外科, 广州 510080;
3.
Department of Chemistry, Texas A&M University, College Station, TX 77840, USA;
4.
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA;
5.
北京师范大学教师教育研究中心, 北京 100875;
6.
中国农业大学水利与土木工程学院, 北京 100083

摘要: 生物体内纷杂多样的代谢过程存在诸多规律可循。本文以亮氨酸代谢为例提供一种教学的新模式。结合其他学科知识从基础代谢角度归纳总结生物代谢中化学原理的内在规律；同时，通过对比不同代谢路径中类似酶在进化上的相似性，发现自然界选择这种代谢方式的规律和原因。这种对比不同代谢路径异同的教学模式，可以帮助学生总结生物代谢不同路径之间的内在规律，加深对相关代谢过程的理解，同时也避免生物化学代谢过程杂乱无章、无迹可寻的错误印象，并增加学习过程的趣味性和思辨性。作为对基础生物化学和生物工程等相关课程中代谢过程教学的改革探索，希望对培养21世纪高素质人才有助。

关键词:

English

Discussion on the Biochemical Similarity in Metabolic Principles Based on Leucine Biosynthesis Pathway

1.
Department of Biology, Texas A & M University, College Station, TX 77840, USA;
2.
Department of Burn Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, P. R. China;
3.
Department of Chemistry, Texas A & M University, College Station, TX 77840, USA;
4.
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Department of Biological Chemistry and Molecular, Pharmacology, Harvard Medical School, Boston, MA 02115, USA;
5.
Center for Teacher Education Research, Beijing Normal University, Beijing 100875, P. R. China;
6.
College of Water Resources & Civil Engineering, China Agricultural University, Beijing 100083, P. R. China
Received Date: 02 August 2018

Abstract: A deep insight into the diverse metabolic pathways gives us the possibility to find the similarities among those processes. This manuscript provides a new paradigm for chemical education using leucine metabolism as the example. Referring concepts from other disciplines, we generalized and summarized the chemical principles involved in the biological metabolisms. Further, patterns and causation behind the predominance of specific metabolic processes were explored through the comparison among homologous enzymes in different pathways to ascertain their evolutionary similarity. This comparison and contrast are intended to help students in summarizing general principles between distinct metabolic pathways, deepening their understanding towards involved metabolic pathways, and preventing the misconception over so-called "chaotic" biochemical metabolic processes. As an attempt of reform in basic biochemistry and bioengineering curricula upon the topic of metabolism, we hope the manuscript can promote students' sense of intrigue, wonder, and curiosity.

Key words:

1. [1] Nelson, D. L.; Cox, M. M.; Lehninger, A. L. Principle of Biochemistry, 4th ed.; W. H. Freeman and Company:New York, 2005.
2. [2] 王镜岩, 朱圣庚, 徐长发. 生物化学. 第3版. 北京:高等教育出版社, 2002:487.
3. [3] 郑集. 普通生物化学. 第4版. 北京:高等教育出版社, 2007.
4. [4] 张洪渊. 生物化学原理. 第1版. 北京:科学出版社, 2006:144.
5. [5] 王联结. 生物化学与分子生物学. 第2版. 北京:科学出版社, 2004:150.
6. [6] 王志鹏, 马新雨, 颜惠娟, 田杰. 大学化学, 2019, 34(1), 24.
7. [7] 王志鹏. 大学化学, 2017, 32(6), 86.
8. [8] Robson, S. A.; Takeuchi, K.; Boeszoermenyi, A.; Coote, P. W.; Dubey, A.; Hyberts, S.; Wagner, G.; Arthanari, H. Nat. Commun. 2018, 9(1), 356.
9. [9] Tamakoshi, M.; Yamagishi, A.; Oshima, T. Gene 1998, 222(1), 125.
10. [10] Gross, S.; Burns, R.; Umbarger, H. Biochemistry 1963, 2(5), 1046.
11. [11] Manikandan, K.; Geerlof, A.; Zozulya, A. V.; Svergun, D. I.; Weiss, M. S. Proteins 2011, 79, 35.
12. [12] Lee, S. G.; Nwumeh, R.; Jez, J. M. J. Biol. Chem. 2016, jbc-M116.
13. [13] Lee, Y. H.; Lee, K.; Hwang, K. Y. Acta Cryst. D. 2014, 70(4), 922.
14. [14] 王志鹏, 邱天, 袁金颖. 化学教育, 2016, 37(6), 1.
15. [15] 马新雨, 王志鹏, 田杰, 蒋振雄, 缑允梓, 颜惠娟, 许兵. 大学化学, 2019, 34(3), 23.
16. [16] 王志鹏. 大学化学, 2017, 32(6), 86.
17. [17] 田杰, 王志鹏, 马新雨, 蒋振雄, 许兵. 大学化学, 2019, 34(2), 81.
18. [18] 王志鹏, 邓耿. 生命的化学, 2015, 35(5), 691.
19. [19] Wang, Z. A.; Kurra, Y.; Wang, X.; Zeng, Y.; Lee, Y. J.; Sharma, V.; Lin, H.; Dai, S. Y.; Liu, W. R. Angew. Chem. Int. Ed. 2017, 56(6), 1643.
20. [20] Tian, J.; Tian, C.; Ding, Y.; Li, Z.; Geng, Q.; Xiahou, Z.; Wang, J.; Hou, W.; Liao, J.; Dong, M. Q. Sci. Rep. 2015, 5, 8360.

扫一扫看文章

计量

PDF下载量: 14
文章访问数: 1129
HTML全文浏览量: 139

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

以亮氨酸合成为例刍议代谢中的生物化学相似性规律

English

Discussion on the Biochemical Similarity in Metabolic Principles Based on Leucine Biosynthesis Pathway

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

以亮氨酸合成为例刍议代谢中的生物化学相似性规律

English

Discussion on the Biochemical Similarity in Metabolic Principles Based on Leucine Biosynthesis Pathway

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs