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Citation: ZHANG Xu, CHEN Yuan. Using Humic Acid for Steady-State and Time-Resolved Fluorescence Experimental Teaching[J]. University Chemistry, ;2017, 32(10): 33-38. doi: 10.3866/PKU.DXHX201706011 shu

Using Humic Acid for Steady-State and Time-Resolved Fluorescence Experimental Teaching

ZHANG Xu ,
CHEN Yuan

School of Resources and Environmental Science, Wuhan University, Wuhan 430072, P. R. China

Humic acid is the major component of natural organic matters, which possesses a complex structure and multiple emission characteristics. Considering the research topics of humic acid or other natural organic matters, humic acid was used for steady-state and time-resolved fluorescence experimental teaching. Students can acquire the knowledge about the Raman scattering and inner filtering effects on fluorescence measurements, the effects of excitation energy on the emission spectrum, and fluorescence lifetime.
- Molecular fluorescence,
- Fluorescence lifetime,
- Humic acid,
- Raman scattering
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