Citation: SUN Yun-Xia, ZHANG Xian-Zheng. Synthesis and Biological Research of Polycationic Oligopeptide: A Research-Based Experiment Recommended for Chemical Biology Majoring Students[J]. University Chemistry, ;2016, 31(3): 42-48. doi: 10.3866/PKU.DXHX20160342 shu

Synthesis and Biological Research of Polycationic Oligopeptide: A Research-Based Experiment Recommended for Chemical Biology Majoring Students

SUN Yun-Xia ^, ,
ZHANG Xian-Zheng

1.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China

Corresponding author: SUN Yun-Xia,
Fund Project: 国家自然科学基金项目(21004046,51473126) (21004046,51473126)

The oligopeptide R₈ was synthesized through the solid phase peptide synthesis (SPPS) protocols, and the molecular weight was measured by MS-ESI. The biological application of R₈ was investigated by agarose gel electrophoresis assay and endocytosis. Through this experiment, students can study and apply organic chemistry, polymer chemistry, and chemical biology, as well as develop their capability for analyzing and resolving problems.
- Amino acid,
- Oligopeptide,
- Peptide,
- Gel electrophoresis
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